Changes in lactic acid content and activity of lactate dehydrogenase in<i>clarias batrachus</i>, exposed to carbaryl

Sharma, Bechan; Gopal, Krishna

doi:10.1080/02772249509358131

Cited by 10 publications

(7 citation statements)

References 13 publications

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“…Some components present in the fertilizer industrial effluent interact together and produce toxicity to aquatic organisms for example the interaction between dissolve oxygen and elevated ammonia has been shown to alter the respiratory physiology of fresh water fish (Pickering and Pottinger 1987). These findings indicated that the inhibitory effect of industrial effluent on the activity of LDH may be mediated via formation of enzyme-inhibitor complex leading to impairment of carbohydrate metabolism (Panepucci et al 1984(Panepucci et al , 1987Copper and Somero 1990;Sharma and Gopal 1995).…”

Section: Resultsmentioning

confidence: 96%

Fertilizer Industry Effluent Induced Biochemical Changes in Fresh water Teleost, Channa striatus (Bloch)

Yadav

Gopesh

Pandey

et al. 2007

Bull Environ Contam Toxicol

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The industrial activities pose threat to the life of aquatic organisms in many ways. This research communication presents an account of the impact of fertilizer industry effluent upon the levels of protein and the activity of lactate dehydrogenase (EC 1.1.1.28, LDH), a terminal key enzyme in glycolytic pathway, in different organs of a fresh water teleost fish, Channa striatus (Bloch). The fish exposed to different sublethal concentrations of fertilizer industry effluent (3.5, 4.7 and 7.0% v/v) equivalent to 1/20th, 1/15th and 1/10th of LC(50) value (70% v/v) for varying treatment periods (96 h and 15 days) exhibited decrease in the level of protein (8-76%) in different organs of the effluent treated fish. At highest effluent concentration (7% v/v) treatment for short (96 h) or long (15 days) duration, the liver of the fish registered significant (p < 0.001) decrease (62-76%) in protein content as compared to control, whereas other organs of the fish showed only 38-52% decrease in the level of protein. The industrial effluent also caused marked reduction in the activity of LDH in different fish tissues when compared to the control. The treatment of fish with 7% effluent concentration for 96 h caused 78% decrease (p < 0.001) in the LDH activity in fish muscle whereas after 15 days the effect was maximum in fish brain as it exhibited 86% decrease (p < 0.001) in LDH activity as compared to control. The effect of effluent on the activity of LDH and protein content in different body tissues of the fish was dependent on concentration and duration of exposure. The significant reductions in the activity of LDH and level of protein in fish tissues due to treatment with the fertilizer industry effluent indicated the possibility of impairments in energy metabolism and protein turnover, respectively, in C. striatus.

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Section: Resultsmentioning

confidence: 96%

Fertilizer Industry Effluent Induced Biochemical Changes in Fresh water Teleost, Channa striatus (Bloch)

Yadav

Gopesh

Pandey

et al. 2007

Bull Environ Contam Toxicol

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“…This indicates that dietary high vitamin C can reduce LDH activity inhibition by deltamethrin. The inhibition in the LDH activity due to deltamethrin exposure could either be due to changes in the conformation of active site [42], formation of enzyme-inhibitor complex leading to impairment of carbohydrate metabolism [43,44] or reduction in de novo synthesis of proteins (enzymes).…”

Section: Discussionmentioning

confidence: 99%

Deltamethrin induced alterations of hematological and biochemical parameters in fingerlings of Catla catla (Ham.) and their amelioration by dietary supplement of vitamin C

Vani

Saharan

Mukherjee

et al. 2011

Pesticide Biochemistry and Physiology

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The present study was carried out to investigate the sub-lethal toxicity of technical grade deltamethrin (a synthetic pyrethroid) of concentration 1.61 lg/L (1/3rd of 96 h LC 50) on hematological and biochemical parameters of catla (Catla catla) fingerlings and its amelioration through dietary vitamin C. The deltamethrin exposed fishes were fed with different levels of supplemented vitamin C such as 50, 250, 500 and 1000 mg/kg diet to see its ameliorating effect by assaying hematological parameters viz. total erythrocyte count (TEC), total leukocyte count (TLC), hemoglobin content (Hb), total serum protein, albumin, globulin, albumin-globulin ratio and biochemical parameters such as lactate dehydrogenase (LDH), acetylcholine esterase (AChE), alanine amino transferase (ALT), aspartate amino transferase (AST), total adenosine triphosphatase (ATPase), magnesium adenosine triphosphatase (Mg 2+-ATPase) and sodium potassium adenosine triphosphatase (Na + , K +-ATPase) activities. The finding of this study showed that deltamethrin had negative effect on the hematological and biochemical parameters of Catla catla. The experimental group, which was exposed to deltamethrin and fed with normal diet showed significantly lower values (P 6 0.05) of all parameters studied except ALT activity. This might be due to possible disruption of hematopoiesis and proteosynthesis. However, the fish fed with varied concentration of vitamin C in diets neutralized the toxic effect of deltamethrin, as evidenced by significantly lowered hematological and biochemical response. Vitamin C @ 1000 mg/kg diet was the most effective in amelioration of harmful effect of deltamethrin on hematological and biochemical parameters of catla fingerlings. The result suggests that vitamin C can be effectively used to neutralize the toxic effect of deltamethrin on catla.

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“…The results are in agreement with the observations reported in freshwater crab exposed to methyl parathion,16 in rat treated with carbofuran14 and in C. batrachus exposed to sublethal concentrations of carbaryl. 15 The results of in-vitro studies conducted to determine the e †ect of carbofuran showed that the pesticide strongly inhibited the activity of LDH isolated from the tissues of C. batrachus (Singh, R. K. & Sharma, B. unpublished data). The Ðnding suggested that the inhibitory e †ect of organocarbamate pesticides on this terminal glycolytic enzyme may be due to the formation of enzymeinhibitor complex, thereby reducing the activity of LDH leading to impairment of carbohydrate metabolism in C. batrachus.…”

Section: Discussionmentioning

confidence: 99%

Carbofuran-induced biochemical changes inClarias batrachus

Singh

Sharma

1998

Pestic. Sci.

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:The e †ect of carbofuran, an organo-carbamate pesticide, upon the level of protein as well as the activity of lactate dehydrogenase (EC 1.1.1.28, LDH) was studied by exposing the teleost Ðsh, Clarias batrachus, to di †erent subacute concentrations (0É01 and 0É02 mg litre~1) for 96 h and 15 days. The results showed a drastic decrease in the protein content in di †erent body organs of the Ðsh. The pesticide also caused a signiÐcant decrease in the level of activity of LDH in di †erent body tissues of the Ðsh, the e †ect being more pronounced in the gills, muscle, brain and liver than in kidney and heart. The decrease in protein content and the activity of LDH in Ðsh tissues was more marked at the higher concentration of the pesticide for the longer duration of treatment. The results suggested that carbofuran has an e †ect at very low concentration (compared to its LC 50 value) possibly at the level of protein metabolism, and also inhibits the activity of LDH, the terminal glycolytic enzyme.1998 SCI ( Pestic. Sci., 53, 285È290 (1998)

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Changes in lactic acid content and activity of lactate dehydrogenase inclarias batrachus, exposed to carbaryl

Cited by 10 publications

References 13 publications

Fertilizer Industry Effluent Induced Biochemical Changes in Fresh water Teleost, Channa striatus (Bloch)

Fertilizer Industry Effluent Induced Biochemical Changes in Fresh water Teleost, Channa striatus (Bloch)

Deltamethrin induced alterations of hematological and biochemical parameters in fingerlings of Catla catla (Ham.) and their amelioration by dietary supplement of vitamin C

Carbofuran-induced biochemical changes inClarias batrachus

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