Hongjiannao groundwater exchange was the largest desert freshwater lake in China (57.25 km 2 in 1986). However, it shrank sharply over the past 34a (1986-2019), with the smallest lake area 31.41 km 2 in 2015. The objective of this study was to use the Landsat images, ASTER GDEM V2 data, and meteorology and statistics data, in combination with the water balance model to calculate the dynamics of water balance elements, quantify and characterize the interannual variations in lakegroundwater exchanges, and analyzed its influencing factors by using the geographical detector. The results showed that in the stable stage (1986-1997), the average rate of the lake area, water level, and lake volume change was-0.26 km 2 /a,-0.0483 m/a, and-0.0009 km 3 /a, respectively. Precipitation, river inflow, and groundwater were 0.0203 km 3 , 0.0485 km 3 , and 0.0098 km 3 , which accounts for the whole input were 25.83%, 61.70%, and 12.47%, respectively, evaporation was 0.0786 km 3. In the reduction stage (1998-2015), the average rate of the lake area, water level, and lake volume change was-1.21 km 2 /a,-0.2422 m/a, and-0.0101 km 3 /a, respectively. Before 2006, precipitation, river inflow, and groundwater were 0.0154 km 3 , 0.0475 km 3 , and-0.0025 km 3 , respectively; from 2006 to 2009, precipitation, river inflow, and groundwater were 0.0143 km 3 , 0.0334 km 3 , and 0.0058 km 3 , respectively; after 2009, precipitation, river inflow, and groundwater were 0.0139 km 3 , 0.0199 km 3 , and 0.0085 km 3 , respectively. Evaporation decreased from 0.0714 km 3 to 0.0480 km 3 from 1998 to 2015. In the growth stage (2016-2019), the average rate of the lake area, water level, and lake volume change were 1.38 km 2 /a, 0.27 m/a, and 0.0088 km 3 /a, respectively. Precipitation, river inflow, and groundwater were 0.0209 km 3 , 0.0005 km 3 , and 0.0373 km 3 , which accounts for the whole input were 46.63 %, 52.12 %, and 1.25 %, respectively, evaporation was 0.0187 km 3. Compared with the stable stage, groundwater in the growth stage reduced from 12.47 % (0.0098 km 3) to only 1.25 % (0.0005 km 3). From 1998 to 2004, Hongjiannao Lake experienced continuous losing conditions (discharge from the lake to groundwater), with a variable exchange volume of up to-0.01582 km 3 in 1999. Through geographical detector analysis, it was found that temperature was the dominant factor from 1988 to 1997, while human factors were the dominant factors from1998 to 2015.