“…The homogenates were centrifuged at 4°C, 10,000×g for 20 min (CTH 2050R, Thermo Fisher Scientific, Waltham, MA, USA), and then filtered through glass microfiber filters (GF/D, Whatman, Maidstone, UK). The total phospholipase, acid lipase, and neutral lipase activities were determined using a fluorescence spectrophotometer (RF-5301, Shimadzu, Kyoto, Japan) according to Shang et al. (2019) .…”