Changes in lipid profiles induced by bisphenol A (BPA) in zebrafish eleutheroembryos during the yolk sac absorption stage

Martínez, Rubén; Navarro‐Martín, Laia; Antro, Morgane Van; Fuertes, Inmaculada; Casado, Marta; Barata, Carlos; Piña, Benjamı́n

doi:10.1016/j.chemosphere.2019.125704

Cited by 32 publications

(8 citation statements)

References 93 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the case of BPA (0.1–4 mg/L), results showed its effects were beyond their well-known estrogenicity, showing complex patterns of toxic effects that included visual disruption and obesogenic effects as evident from the appearance of yolk sac malabsorption syndrome and lipid and metabolism disruption at the transcriptomic and metabolomic level ( Martínez et al, 2019a ; Ortiz-Villanueva et al, 2017 ). Several mechanisms have been proposed to explain these observed effects on lipid metabolism, which could differ along the different lipid classes ( Martínez et al, 2020a ), but also disruption in some energetic metabolic processes seemed to be maintained during part of the life cycle ( Martínez et al, 2020b ). On the other hand, TBT alteration of the yolk sac area was partially attributed to developmental disruption, since TBT exposures (1–32 mg/L) caused a general developmental delay (diapause-arrest effect) which affected steroids and cell cycle metabolic pathways ( Martínez et al, 2020c ).…”

Section: Case Study 1: Metabolic Syndrome and Non-eats Modalitiesmentioning

confidence: 99%

Emerging concepts and opportunities for endocrine disruptor screening of the non-EATS modalities

Martyniuk

Martínez

Navarro‐Martín

et al. 2022

Environmental Research

Self Cite

View full text Add to dashboard Cite

Section: Case Study 1: Metabolic Syndrome and Non-eats Modalitiesmentioning

confidence: 99%

Emerging concepts and opportunities for endocrine disruptor screening of the non-EATS modalities

Martyniuk

Martínez

Navarro‐Martín

et al. 2022

Environmental Research

Self Cite

View full text Add to dashboard Cite

“…Table 2 details separation conditions for total lipid classes which cover a wide range of polarity. In addition to PL and SL (ceramides), different NL subclasses have been separated using the conditions described there (diacylglycerides, DG; triacylglicerides, TG; wax esters; sterols; cholesterol (Chol), chosteryl esters (CholE), FFA, hydroxy-FA, ecdysteroids) from different biological samples, using either isocratic [13,17,[28][29][30][31][32] or sequential development [33]. Table 2 In general, normal-phase was mostly used for separations, in the form of silicagel HPTLC or Lichrospher plates.…”

Section: Tablementioning

confidence: 99%

Lipidomic studies based on high-performance thin-layer chromatography

Cebolla

Jarne

Membrado

et al. 2022

JPC-J Planar Chromat

View full text Add to dashboard Cite

The last triennium has shown a significant contribution of High-Performance Thin-Layer Chromatography (HPTLC) to lipidomics. HPTLC separation in combination with radioand /or Ultraviolet (UV)-Fluorescence (FL) densitometry was the technique of choice for tracking the transport of phospholipids among different cellular compartments in a number of biological systems, e.g., Gram-negative bacteria, yeast membranes, endoplasmic reticulum/mitochondrial membrane interface, and also to monitor the lipid transfer activity of a protein. Likewise, a significant number of HPTLC methods were developed to determine variations in the content of different lipid classes and subclasses after using genetic knockouts of cells. Radiolabeled control cells and cells whose genes were deleted using genomic editing allowed to study by HPTLC the effect of the disruption of the related proteins on the corresponding biosynthetic pathways.As well, direct interface-based coupling of HPTLC to Mass Spectrometry (MS) using mostly Electrospray (ESI) ionization and a variety of mass analyzers, has gained new momentum in this period. Obtaining a large amount of information online in a very short time from complex biological samples, and the structural identification of target and non-target, unknown lipids, make this technique a useful tool for lipidomics. Quantitative issues related to HPTLC-MS are also discussed in this work.

show abstract

“…Descriptive analysis [22] Lobasso Together with other techniques, HPTLC contributed to demonstrate that differences in some subclasses with time and BPA-related patterns depended on the unsaturation degree (mostly DG and PC) and fatty acid chain length (mostly DG and PC) [98] Contribution to the elucidation of the physiological role of Spf1p, a P5A ATPase, an enzyme which are involved in regulation of homeostasis in the endoplasmic reticulum [99] Mollerup a Gradient AMD.…”

Section: Sq With External Calibrationmentioning

confidence: 99%

“…External calibration for SQ analysis using densitometry has usually been performed using representative standards of the corresponding lipid sub-class. [10,19,27,28,[34][35][36][37]40,46,52,73,79,85,86,[95][96][97][98] A response factor system for quantitation was employed for a rapid and sensitive determination of MG, DG, TG and FFA in E471 emulsifiers by HPTLC with fluorescence densitometry using plates pre-chromatographically impregnated with primuline. [87] Calibration was done with 1,2-distearin and the amounts of lipid classes were calculated using response factors.…”

Section: Semi-quantave and Quantave Analysesmentioning

confidence: 99%

Scanning densitometry and mass spectrometry for HPTLC analysis of lipids: The last 10 years

Cebolla

Jarne

Vela

et al. 2021

Journal of Liquid Chromatography & Related Technologies

View full text Add to dashboard Cite

This work is a review on HPTLC contribuon to lipid analysis in complex matrices, in the period from 2010 to now, lapse of me where hyphenaon with other techniques, especially Mass Spectrometry, has experienced an important growth.Scanning densitometry (SD)-UV/FL of separated lipids, as the core of detecon and centerpiece for hyphenaon, and its coupling with MS using so ionizaon techniques (ESI, APCI, MALDI, DESI and others), are the central axis of this work. The occasional intercalaon in this coupling of an on-plate biological assay for eect-direct analysis (EDA) of lipids, as well as the combinaon of SD with radiodensitometry (RD) using isotopically labeled-lipids are also covered topics.The described techniques make possible to develop strategies for obtaining qualitave and dierent levels of quantave informaon, including untargeted lipids species.HPTLC of lipids has been used for: comparave purposes; ngerprinng; semi-quantave determinaon, idencaon of species; or quantave determinaon of a given individual lipid. Goals in molecular biology and biochemistry-related samples have been: preparave isolaons; control of purity; vericaon of metabolic products; on-plate biological assays; proles in cells; analysis of products from the cellular metabolism; measurement of enzymac acvies; monitoring lipid transport across membranes or at biological interfaces; or monitoring lipid dynamics.

show abstract

Changes in lipid profiles induced by bisphenol A (BPA) in zebrafish eleutheroembryos during the yolk sac absorption stage

Cited by 32 publications

References 93 publications

Emerging concepts and opportunities for endocrine disruptor screening of the non-EATS modalities

Emerging concepts and opportunities for endocrine disruptor screening of the non-EATS modalities

Lipidomic studies based on high-performance thin-layer chromatography

Scanning densitometry and mass spectrometry for HPTLC analysis of lipids: The last 10 years

Contact Info

Product

Resources

About