Changes in Lung Permeability Correlate With Lung Histology in a Chronic Exposure Model

Hays, Allison M.; Parliman, G.; Pfaff, John K.; Lantz, R. Clark; Tinajero, Julieta; Tollinger, Brian J.; Hall, Jack N.; Witten, Mark L.

doi:10.1177/074823379501100303

Cited by 66 publications

(54 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Histological analysis can also provide information about morphological changes to tissue that indicate stress and injury. This type of analysis was used to demonstrate injury to tissues that directly contact JP-8 fuel such as lung and skin (Hays et al, 1995;Robledo et al, 2000;Gallucci et al, 2004). However, little information is available concerning the systemic toxicity of JP-8 through the evaluation of tissue injury with histological analyses.…”

Section: Introductionmentioning

confidence: 99%

Stress induced in heart and other tissues by rat dermal exposure to JP-8 fuel

et al. 2005

View full text Add to dashboard Cite

Limited information is available regarding the development of systemic organ stress by dermal exposure to JP-8 fuel. In this study, the systemic stress potential of this fuel is evaluated in a rat model subjected to dermal applications of JP-8 for 7 days at 300 microl per day. Tissue histology indicated that JP-8 induces morphological alterations that suggest that tissue stress in the heart is more substantial than stress in the kidney and liver. Immunoblot analysis of tissues revealed increased levels of the inducible heat shock protein 70 (HSP70) in the heart, kidney, and liver after this dermal JP-8 exposure. This exposure also leads to increased levels of heme oxygenase-1 (HO-1/HSP3) in the liver. Additionally during this exposure, a negative regulator of inflammation, IkappaBalpha (inhibitor of NF-kappaB), was increased in the liver, slightly increased in the kidney, and not increased in the heart. Two regions of the rat brain were also examined and HSP70 and IkappaBalpha were increased in the cerebellum but not significantly increased in the cortex. This study indicates dermal JP-8 exposure causes systemic alterations that are associated with cytoprotective activities (e.g., in the liver) as well as potentially toxic mechanisms (heart and kidney).

show abstract

Section: Introductionmentioning

confidence: 99%

Stress induced in heart and other tissues by rat dermal exposure to JP-8 fuel

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Morphological evaluation after acute JP-8 aerosol exposures showed lesions following a range of doses up to 113 mg/m 3 (4,17,19). Subsequent in vitro experiments in our labs explored the role of epithelial barrier function upon JP-8 exposure.…”

Section: Morphological Evaluation-histology and Micrographymentioning

confidence: 99%

“…Initial in vivo experiments in our labs revealed that aerosol exposure results in the targeting of distal airways as shown by enhanced respiratory permeability to 99m TcDTPA (4,17,18). Morphological evaluation after acute JP-8 aerosol exposures showed lesions following a range of doses up to 113 mg/m 3 (4,17,19).…”

Section: Morphological Evaluation-histology and Micrographymentioning

confidence: 99%

Correlation Between In Vivo and In Vitro Pulmonary Responses to Jet Propulsion Fuel-8 Using Precision-Cut Lung Slices and a Dynamic Organ Culture System

2003

Self Cite

View full text Add to dashboard Cite

In tissue slice models, interactions between the heterogeneous cell types comprising the lung parenchyma are maintained thus providing a controlled system for the study of pulmonary toxicology in vitro. However, validation of the model in vitro system must be affirmed. Previous reports, in in vivo systems, have demonstrated that Clara cells and alveolar type II cells are the targets following inhalation of JP-8 jet fuel. We have utilized the lung slice model to determine if cellular targets are similar following in vitro exposure to JP-8. Agar-filled adult rat lung explants were cored and precision cut, using the Brendel/Vitron tissue slicer. Slices were cultured on titanium screens located as half-cylinders in cylindrical Teflon cradles that were loaded into standard scintillation vials and incubated at 37 • C. Slices were exposed to JP-8 jet fuel (0.5 mg/ml, 1.0 mg/ml, and 1.5 mg/ml in medium) for up to 24 hours. We determined ATP content using a luciferin-luciferase bioluminescent assay. No significant difference was found between the JP-8 jet fuel doses or time points, when compared to controls. Results were correlated with structural alterations following aerosol inhalation of JP-8. As a general observation, ultrastructural evaluation of alveolar type II cells revealed an apparent increase in the number and size of surfactant secreting lamellar bodies that was JP-8 jet fuel-dose dependent. These results are similar to those observed following aerosol inhalation exposure. Thus, the lung tissue slice model appears to mimic in vivo effects of JP-8 and therefore is a useful model system for studying the mechanisms of lung injury following JP-8 exposure.

show abstract

“…Furthermore, no coal ash particles solubility in UPW has been determined. (Hays et al, 1995) has previously determined that increased clearance of 99m Tc-DTPA correlates with increased pathological injury. Furthermore, (Witten et al, 1985) has demonstrated that pulmonary clearance of 99m Tc-DTPA is an extremely sensitive marker of lung injury.…”

Section: Characteristics Of Re-suspended Ash Particlesmentioning

confidence: 99%

NOx, FINE PARTICLE AND TOXIC METAL EMISSIONS FROM THE COMBUSTION OF SEWAGE SLUDGE/COAL MIXTURES: A SYSTEMATIC ASSESSMENT

Wendt¹

2002

View full text Add to dashboard Cite

This research project focuses on pollutants from the combustion of mixtures of dried municipal sewage sludge (MSS) and coal. The objective is to determine the relationship between 1) fraction sludge in the sludge/coal mixture, and 2) combustion conditions on a) NOx concentrations in the exhaust, b) the size segregated fine and ultra-fine particle composition in the exhaust, and c) the partitioning of toxic metals between vapor and condenses phases, within the process. The proposed study will be conducted in concert with an existing ongoing research on toxic metal partitioning mechanisms for very well characterized pulverized coals alone. Both high NOx and low NOx combustion conditions will be investigated (unstaged and staged combustion). Tradeoffs between CO 2 control, NO x control, and inorganic fine particle and toxic metal emissions will be determined.Previous research has yielded data on trace metal partitioning for MSS by itself, with natural gas assist, for coal plus MSS combustion together, and for coal alone. We have re-evaluated the inhalation health effects of ash aerosol from combustion of MSS both by itself and also together with coal. We have concluded that ash from the co-combustion of MSS and coal is very much worse from an inhalation health point of view, than ash from either MSS by itself or coal by itself. The reason is that ZnO is not the "bad actor" as had been suspected before, but the culprit is, rather, sulfated Zn. The MSS supplies the Zn and the coal supplies the sulfur, and so it is the combination of coal and MSS that makes that process environmentally bad. If MSS is to be burned, it should be burned without coal, in the absence of sulfur.4

show abstract

Changes in Lung Permeability Correlate With Lung Histology in a Chronic Exposure Model

Cited by 66 publications

References 6 publications

Stress induced in heart and other tissues by rat dermal exposure to JP-8 fuel

Stress induced in heart and other tissues by rat dermal exposure to JP-8 fuel

Correlation Between In Vivo and In Vitro Pulmonary Responses to Jet Propulsion Fuel-8 Using Precision-Cut Lung Slices and a Dynamic Organ Culture System

NOx, FINE PARTICLE AND TOXIC METAL EMISSIONS FROM THE COMBUSTION OF SEWAGE SLUDGE/COAL MIXTURES: A SYSTEMATIC ASSESSMENT

Contact Info

Product

Resources

About