Changes in membrane biophysical properties induced by the Budesonide/Hydroxypropyl-β-cyclodextrin complex

Santos, Andreia G. dos; Bayiha, Jules César; Dufour, Gilles; Cataldo, Didier; Évrard, Brigitte; Silva, Liana C.; Deleu, Magali; Mingeot‐Leclercq, Marie‐Paule

doi:10.1016/j.bbamem.2017.06.010

Cited by 19 publications

(16 citation statements)

References 60 publications

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“…Membrane permeabilization was followed as described previously 78 . Release of entrapped calcein at self-quenching concentrations from LUV composed by PLPC/sitosterol (80/20–16 µM) can be monitored by the fluorescence increase upon dilution following their leakage from the vesicles.…”

Section: Methodsmentioning

confidence: 99%

Synthetic Rhamnolipid Bolaforms trigger an innate immune response in Arabidopsis thaliana

Luzuriaga-Loaiza

Schellenberger

Gaetano

et al. 2018

Sci Rep

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Stimulation of plant innate immunity by natural and synthetic elicitors is a promising alternative to conventional pesticides for a more sustainable agriculture. Sugar-based bolaamphiphiles are known for their biocompatibility, biodegradability and low toxicity. In this work, we show that Synthetic Rhamnolipid Bolaforms (SRBs) that have been synthesized by green chemistry trigger Arabidopsis innate immunity. Using structure-function analysis, we demonstrate that SRBs, depending on the acyl chain length, differentially activate early and late immunity-related plant defense responses and provide local increase in resistance to plant pathogenic bacteria. Our biophysical data suggest that SRBs can interact with plant biomimetic plasma membrane and open the possibility of a lipid driven process for plant-triggered immunity by SRBs.

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Section: Methodsmentioning

confidence: 99%

Synthetic Rhamnolipid Bolaforms trigger an innate immune response in Arabidopsis thaliana

Luzuriaga-Loaiza

Schellenberger

Gaetano

et al. 2018

Sci Rep

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“…LUVs of fish-like, holothuroid-like and sterol-free (Table 1 ) were prepared in a saline-Tris-HCl buffer (600 mM NaCl, 10 mM TRIS, pH 7.5) with a self-quenching concentration of calcein (10 mM). The un-encapsulated dye was removed on a gel column of Sephadex G75 42 .…”

Section: Methodsmentioning

confidence: 99%

How different sterols contribute to saponin tolerant plasma membranes in sea cucumbers

Claereboudt

Eeckhaut

Lins

et al. 2018

Sci Rep

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Sea cucumbers produce saponins as a chemical defense mechanism, however their cells can tolerate the cytotoxic nature of these chemicals. To elucidate the molecular mechanisms behind this tolerance a suite of complementary biophysical tools was used, firstly using liposomes for in vitro techniques then using in silico approaches for a molecular-level insight. The holothuroid saponin Frondoside A, caused significantly less permeabilization in liposomes containing a Δ7 holothuroid sterol than those containing cholesterol and resulted in endothermic interactions versus exothermic interactions with cholesterol containing liposomes. Lipid phases simulations revealed that Frondoside A has an agglomerating effect on cholesterol domains, however, induced small irregular Δ7 sterol clusters. Our results suggest that the structural peculiarities of holothuroid sterols provide sea cucumbers with a mechanism to mitigate the sterol-agglomerating effect of saponins, and therefore to protect their cells from the cytotoxicity of the saponins they produce.

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“…To give insight on the molecular mechanisms involved in the protective effect of BUD:HPBCD and HPBCD, the potential role of cholesterol on ROS generation and PI3/Akt phosphorylation induced by H 2 O 2 + LPS as well as the protective effects of the BUD:HPβCD complex and HPβCD were investigated. The rationale was derived from the ability of cyclodextrins to interact with cholesterol [ 35 ], the effects of BUD:HPβCD and HPβCD on the biophysical membrane properties of cholesterol-enriched domains [ 27 ], the importance of lipid-ordered domains enriched in cholesterol in membrane called rafts for ROS generation [ 36 , 37 ] and PI3K/Akt signaling [ 23 , 26 ].…”

Section: Resultsmentioning

confidence: 99%

“…Cholesterol is largely known for its effect on biophysical membrane properties and cholesterol-enriched domains are linked to membrane signaling [ 23 , 24 , 25 , 26 ] including pathways involved in PI3K/Akt signaling and inflammation processes. On giant unilamellar vesicles (GUVs) and lipid monolayers, BUD:HPβCD induced the disruption of cholesterol-enriched raft-like liquid ordered domains—an increase in membrane permeability and fluidity [ 27 ]. Except for membrane fluidity, all these effects were enhanced when HPβCD was complexed with budesonide as compared with HPβCD [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

“…On giant unilamellar vesicles (GUVs) and lipid monolayers, BUD:HPβCD induced the disruption of cholesterol-enriched raft-like liquid ordered domains—an increase in membrane permeability and fluidity [ 27 ]. Except for membrane fluidity, all these effects were enhanced when HPβCD was complexed with budesonide as compared with HPβCD [ 27 ]. On cellular models, this could involve signal transduction pathways such as ROS generation, inflammatory cytokines expression and cell death.…”

Section: Introductionmentioning

confidence: 99%

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The Budesonide-Hydroxypropyl-β-Cyclodextrin Complex Attenuates ROS Generation, IL-8 Release and Cell Death Induced by Oxidant and Inflammatory Stress. Study on A549 and A-THP-1 Cells

et al. 2020

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Synthetic glucocorticoids such as budesonide (BUD) are potent anti-inflammatory drugs commonly used to treat patients suffering from chronic inflammatory diseases. A previous animal study reported a higher anti-inflammatory activity with a 2-hydroxypropyl-β-cyclodextrin (HPβCD)-based formulation of BUD (BUD:HPβCD). This study investigated, on cellular models (A549 and A-THP-1), the effect of BUD:HPβD in comparison with BUD and HPβCD on the effects induced by oxidative and inflammatory stress as well as the role of cholesterol. We demonstrated the protective effect afforded by BUD:HPβCD against cytotoxicity and ROS generation induced by oxidative and inflammatory stress. The effect observed for BUD:HPβCD was comparable to that observed with HPβCD with no major effect of cholesterol content. We also demonstrated (i) the involvement of the canonical molecular pathway including ROS generation, a decrease in PI3K/Akt activation, and decrease in phosphorylated/unphosphorylated HDAC2 in the effect induced by BUD:HPβCD, (ii) the maintenance of IL-8 decrease with BUD:HPβCD, and (iii) the absence of improvement in glucocorticoid insensitivity with BUD:HPβCD in comparison with BUD, in conditions where HDAC2 was inhibited. Resulting from HPβCD antioxidant and anticytotoxic potential and protective capacity against ROS-induced PI3K/Akt signaling and HDAC2 inhibition, BUD:HPβCD might be more beneficial than BUD alone in a context of concomitant oxidative and inflammatory stress.

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Changes in membrane biophysical properties induced by the Budesonide/Hydroxypropyl-β-cyclodextrin complex

Cited by 19 publications

References 60 publications

Synthetic Rhamnolipid Bolaforms trigger an innate immune response in Arabidopsis thaliana

Synthetic Rhamnolipid Bolaforms trigger an innate immune response in Arabidopsis thaliana

How different sterols contribute to saponin tolerant plasma membranes in sea cucumbers

The Budesonide-Hydroxypropyl-β-Cyclodextrin Complex Attenuates ROS Generation, IL-8 Release and Cell Death Induced by Oxidant and Inflammatory Stress. Study on A549 and A-THP-1 Cells

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