Changes in Membrane Fatty Acid Composition of Pseudomonas aeruginosa in Response to UV-C Radiations

Ghorbal, Salma Kloula Ben; Chatti, Abdelwaheb; Sethom, Mohamed Marwan; Maalej, Lobna; Mihoub, Mouadh; Kefacha, Sana; Feki, Moncef; Landoulsi, Ahmed; Hassen, Abdennaceur

doi:10.1007/s00284-013-0342-5

Cited by 30 publications

(23 citation statements)

References 21 publications

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“…This holds true especially for mechanisms which relay on the hydrophobic thickness of membranes like the ratio and transition between left- and right-handed interface e.g., of EphA2 [44] or EpHA1 [43]. Translating our findings for the different membranes into a biological context suggests that modulation of dimerization interfaces of transmembrane proteins can be achieved by changes of the lipid composition, which is a common stresses reaction [52,53,54]. Such dependence of protein action and membrane dynamics has for example been described for Hik33 [55,56,57] or DesK [58].…”

Section: Discussionmentioning

confidence: 78%

The Influence of Fatty Acids on the GpA Dimer Interface by Coarse-Grained Molecular Dynamics Simulation

Flinner¹,

Mirus²,

Schleiff³

2014

IJMS

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The hydrophobic thickness of membranes, which is manly defined by fatty acids, influences the packing of transmembrane domains of proteins and thus can modulate the activity of these proteins. We analyzed the dynamics of the dimerization of Glycophorin A (GpA) by molecular dynamics simulations to describe the fatty acid dependence of the transmembrane region assembly. GpA represents a well-established model for dimerization of single transmembrane helices containing a GxxxG motif in vitro and in silico. We performed simulations of the dynamics of the NMR-derived dimer as well as self-assembly simulations of monomers in membranes composed of different fatty acid chains and monitored the formed interfaces and their transitions. The observed dimeric interfaces, which also include the one known from NMR, are highly dynamic and converted into each other. The frequency of interface formation and the preferred transitions between interfaces similar to the interface observed by NMR analysis strongly depend on the fatty acid used to build the membrane. Molecular dynamic simulations after adaptation of the helix topology parameters to better represent NMR derived structures of single transmembrane helices yielded an enhanced occurrence of the interface determined by NMR in molecular dynamics simulations. Taken together we give insights into the influence of fatty acids and helix conformation on the dynamics of the transmembrane domain of GpA.

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Section: Discussionmentioning

confidence: 78%

The Influence of Fatty Acids on the GpA Dimer Interface by Coarse-Grained Molecular Dynamics Simulation

Flinner¹,

Mirus²,

Schleiff³

2014

IJMS

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“…Even fatty acid chain identity is highly variable; cardiolipin is characterized by a high degree of chain unsaturation (14,(55)(56)(57) and a global negative spontaneous curvature (58 -60). In turn, cardiolipin leads to highly bent structures and domains in disordered phases.…”

Section: Discussionmentioning

confidence: 99%

Negatively Charged Lipids as a Potential Target for New Amphiphilic Aminoglycoside Antibiotics

Sautrey¹,

Khoury²,

Santos³

et al. 2016

Journal of Biological Chemistry

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Bacterial membranes are highly organized, containing specific microdomains that facilitate distinct protein and lipid assemblies. Evidence suggests that cardiolipin molecules segregate into such microdomains, probably conferring a negative curvature to the inner plasma membrane during membrane fission upon cell division. 3,6-Dinonyl neamine is an amphiphilic aminoglycoside derivative active against Pseudomonas aeruginosa, including strains resistant to colistin. The mechanisms involved at the molecular level were identified using lipid models (large unilamellar vesicles, giant unilamelllar vesicles, and lipid monolayers) that mimic the inner membrane of P. aeruginosa. The study demonstrated the interaction of 3,6-dinonyl neamine with cardiolipin and phosphatidylglycerol, two negatively charged lipids from inner bacterial membranes. This interaction induced membrane permeabilization and depolarization. Lateral segregation of cardiolipin and membrane hemifusion would be critical for explaining the effects induced on lipid membranes by amphiphilic aminoglycoside antibiotics. The findings contribute to an improved understanding of how amphiphilic aminoglycoside antibiotics that bind to negatively charged lipids like cardiolipin could be promising antibacterial compounds.

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“…This could be performed by the alteration of fatty acid composition, their chain length, and phospholipid composition [22]. Most bacteria are resisting the fluidizing effect of hydrophobic compounds by changing their membrane composition to reduce fluidity and to maintain balance between bilayer and nonbilayer forming phospholipids [23]. These adaptation mechanisms rely on a modification of the membrane phospholipids.…”

Section: Adaptation Mechanisms Of Bacterial Strainsmentioning

confidence: 99%

Response Mechanisms of Bacterial Degraders to Environmental Contaminants on the Level of Cell Walls and Cytoplasmic Membrane

Murínová

Dercová

2014

International Journal of Microbiology

154

106

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Bacterial strains living in the environment must cope with the toxic compounds originating from humans production. Surface bacterial structures, cell wall and cytoplasmic membrane, surround each bacterial cell and create selective barriers between the cell interior and the outside world. They are a first site of contact between the cell and toxic compounds. Organic pollutants are able to penetrate into cytoplasmic membrane and affect membrane physiological functions. Bacteria had to evolve adaptation mechanisms to counteract the damage originated from toxic contaminants and to prevent their accumulation in cell. This review deals with various adaptation mechanisms of bacterial cell concerning primarily the changes in cytoplasmic membrane and cell wall. Cell adaptation maintains the membrane fluidity status and ratio between bilayer/nonbilayer phospholipids as well as the efflux of toxic compounds, protein repair mechanisms, and degradation of contaminants. Low energy consumption of cell adaptation is required to provide other physiological functions. Bacteria able to survive in toxic environment could help us to clean contaminated areas when they are used in bioremediation technologies.

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Changes in Membrane Fatty Acid Composition of Pseudomonas aeruginosa in Response to UV-C Radiations

Cited by 30 publications

References 21 publications

The Influence of Fatty Acids on the GpA Dimer Interface by Coarse-Grained Molecular Dynamics Simulation

The Influence of Fatty Acids on the GpA Dimer Interface by Coarse-Grained Molecular Dynamics Simulation

Negatively Charged Lipids as a Potential Target for New Amphiphilic Aminoglycoside Antibiotics

Response Mechanisms of Bacterial Degraders to Environmental Contaminants on the Level of Cell Walls and Cytoplasmic Membrane

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