Changes in metabolic and physiological biomarkers in Mangalarga Marchador horses with induced obesity

Ribeiro, Rodrigo Martins; Ribeiro, Débora Stremel; Cota, Leticia O.; Leme, Fabíola Oliveira Paes; Carvalho, Armando Mattos; Faleiros, Rafael Resende

doi:10.1016/j.tvjl.2021.105627

Cited by 7 publications

(5 citation statements)

References 14 publications

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“…In the present study, no differences in triglyceride or non-esterified fatty acid concentrations were observed, although more detailed assays, such as mass spectrometry utilized in our previous study, could have identified alterations in the fatty acid profile that may have contributed to observed outcomes. Studies that utilized similar methodology as performed in this study, reported no differences for triglyceride and non-esterified fatty acid concentrations in plasma from normal-weight and obese pregnant mares 17 ; or in mature horses after dietary induction of obesity 49 .…”

Section: Discussionsupporting

confidence: 50%

Follicular metabolic alterations are associated with obesity in mares and can be mitigated by dietary supplementation

Catandi,

Fresa,

Cheng

et al. 2024

Sci Rep

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Obesity is a growing concern in human and equine populations, predisposing to metabolic pathologies and reproductive disturbances. Cellular lipid accumulation and mitochondrial dysfunction play an important role in the pathologic consequences of obesity, which may be mitigated by dietary interventions targeting these processes. We hypothesized that obesity in the mare promotes follicular lipid accumulation and altered mitochondrial function of oocytes and granulosa cells, potentially contributing to impaired fertility in this population. We also predicted that these effects could be mitigated by dietary supplementation with a combination of targeted nutrients to improve follicular cell metabolism. Twenty mares were grouped as: Normal Weight [NW, n = 6, body condition score (BCS) 5.7 ± 0.3], Obese (OB, n = 7, BCS 7.7 ± 0.2), and Obese Diet Supplemented (OBD, n = 7, BCS 7.7 ± 0.2), and fed specific feed regimens for ≥ 6 weeks before sampling. Granulosa cells, follicular fluid, and cumulus-oocyte complexes were collected from follicles ≥ 35 mm during estrus and after induction of maturation. Obesity promoted several mitochondrial metabolic disturbances in granulosa cells, reduced L-carnitine availability in the follicle, promoted lipid accumulation in cumulus cells and oocytes, and increased basal oocyte metabolism. Diet supplementation of a complex nutrient mixture mitigated most of the metabolic changes in the follicles of obese mares, resulting in parameters similar to NW mares. In conclusion, obesity disturbs the equine ovarian follicle by promoting lipid accumulation and altering mitochondrial function. These effects may be partially mitigated with targeted nutritional intervention, thereby potentially improving fertility outcomes in the obese female.

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Section: Discussionsupporting

confidence: 50%

Follicular metabolic alterations are associated with obesity in mares and can be mitigated by dietary supplementation

Catandi,

Fresa,

Cheng

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In the present study, no differences in triglyceride or non-esterified fatty acid concentrations were observed, although more detailed assays, such as mass spectrometry utilized in our previous study, could have identified alterations in the fatty acid profile that may have contributed to observed outcomes. Studies that utilized similar methodology as performed in this study, reported no differences for triglyceride and nonesterified fatty acid concentrations in plasma from normal-weight and obese pregnant mares 17 ; or in mature horses after dietary induction of obesity 49 .…”

Section: Discussionsupporting

confidence: 50%

Follicular metabolic alterations are associated with obesity in mares and can be mitigated by dietary supplementation

Catandi,

Fresa,

Cheng

et al. 2024

Preprint

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Obesity is a growing concern in human and equine populations, predisposing to metabolic pathologies and reproductive disturbances. Cellular lipid accumulation and mitochondrial dysfunction play an important role in the pathologic consequences of obesity, which may be mitigated by dietary interventions targeting these processes. We hypothesized that obesity in the mare promotes follicular lipid accumulation and altered mitochondrial function of oocytes and granulosa cells, potentially contributing to impaired fertility in this population. We also predicted that these effects could be mitigated by dietary supplementation with a combination of targeted nutrients to improve follicular cell metabolism. Twenty mares were grouped as: Normal Weight (NW, n = 6, BCS 5.7±0.3), Obese (OB, n = 7, BCS 7.7±0.2), and Obese Diet Supplemented (OBD, n = 7, BCS 7.7±0.2), and fed specific feed regimens for ≥ 6 weeks before sampling. Granulosa cells, follicular fluid, and cumulus-oocyte complexes were collected from follicles ≥ 35 mm during estrus and after induction of maturation. Obesity promoted several mitochondrial metabolic disturbances in granulosa cells, reduced L-carnitine availability in the follicle, promoted lipid accumulation in cumulus cells and oocytes, and increased basal oocyte metabolism. Diet supplementation of a complex nutrient mixture mitigated most of the metabolic changes in the follicles of obese mares, resulting in parameters similar to NW mares. In conclusion, obesity disturbs the equine ovarian follicle by promoting lipid accumulation and altering mitochondrial function. These effects may be partially mitigated with targeted nutritional intervention, thereby potentially improving fertility outcomes in the obese female.

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“…The MM horses that received high concentrations of NSCs had lesions in the lamellar tissue, unsurprising since hormonal imbalances were previously identified [ 16 , 18 , 23 , 24 ]. The lesions included abnormal keratinization of the abaxial border of several PDLs, PEL discontinuities, and condensed nuclei of SELBCs with loss of their orientation perpendicular to the BM.…”

Section: Discussionmentioning

confidence: 99%

“…The length of SEL was shorter in MM in all regions of the PEL when compared with the other groups, contradicting the results found in natural cases of hyperinsulinaemia [ 22 ], and in models of insulin [ 21 , 25 ] or oligofructose [ 21 ] inducted laminitis, where there is stretching of the SEL. Since MM horses in this study were in the early stages of insulin dysregulation [ 23 ], it is believed that the shortening of SEL extent is possibly associated with the process of sinking DP [ 16 ], which may precede the characteristic stretching seen in horses artificially exposed to much higher blood insulin concentrations [ 21 , 25 ] and in horses in more advanced stages of hyperinsulinaemia, which already have clinical evidence of laminitis [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Histological comparison of the lamellar tissue of Iberian origin breed horses created in semi-feral conditions or in an intensive system

et al. 2023

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Although the external conformation of wild horse hooves has been proposed as an ideal model for domesticated modern horses, histological signs of laminitis have been reported among them. With the hypothesis that the lamellar tissue of horses of Iberian origin raised in semi-feral is healthier than those raised in an intensive management system (stall confinement and high-calorie diet intake), the objective was to compare their lamellar tissues. Lamellar tissue samples were taken from the forelimb hoof of eight domesticated Mangalarga Marchador (MM) horses and from six semi-feral Marajoara (MJ) and Puruca (MP) horses. Primary epidermal lamella (PEL) and secondary epidermal lamella (SEL) were measured (length and width) in several regions, and their shapes were morphologically classified into different types. Breed groups were compared using analysis of variance, followed by Tukey or Dunn tests (P<0.05). Early signs of laminitis such as abnormal keratinization of the abaxial primary dermal lamella, tapered tips of the axial PELs, pointed tips of the SEL, nuclei condensation, and abnormal spatial orientation of the secondary epidermal basal cells (SELBC), were seen only in MM horses. MP horses had a greater interface of epidermis/dermis contact than MJ horses and more rounded nuclei in the round SELBC than MM horses. In agreement with the study hypothesis, semi-feral MJ and MP horses’ lamellae were classified as healthier than MM horses, which showed early signs of endocrinopathic laminitis.

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Changes in metabolic and physiological biomarkers in Mangalarga Marchador horses with induced obesity

Cited by 7 publications

References 14 publications

Follicular metabolic alterations are associated with obesity in mares and can be mitigated by dietary supplementation

Follicular metabolic alterations are associated with obesity in mares and can be mitigated by dietary supplementation

Follicular metabolic alterations are associated with obesity in mares and can be mitigated by dietary supplementation

Histological comparison of the lamellar tissue of Iberian origin breed horses created in semi-feral conditions or in an intensive system

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