Changes in methylation of genomic DNA from chicken immune organs in response to H5N1 influenza virus infection

Zhang, Y.H.; Meng, Jin; Gao, Yan; Zhang, J.Y.; Niu, Shichao; Yu, Xianzhong; Li, Yanbing; Guan, Yuntao; Sun, Boxing; Zhao, Zuowei

doi:10.4238/gmr.15037382

Cited by 16 publications

(7 citation statements)

References 37 publications

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“…Histone modifications in the respiratory tract have been associated with respiratory inflammation and infection, including SARS-CoV [117,118]. Influenza induces modifications that alter the expression of some pro-inflammatory cytokines and interleukin genes, but this is largely associated with DNA methylation during viral replication [119][120][121][122][123][124][125][126]. Thus, the epigenetic changes we observed in T cells are not likely to induce by the influenza virus itself.…”

Section: Discussionmentioning

confidence: 79%

Mapping Influenza-Induced Posttranslational Modifications on Histones from CD8+ T Cells

Rezinciuc

Tian

et al. 2020

Viruses

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T cell function is determined by transcriptional networks that are regulated by epigenetic programming via posttranslational modifications (PTMs) to histone proteins and DNA. Bottom-up mass spectrometry (MS) can identify histone PTMs, whereas intact protein analysis by MS can detect species missed by bottom-up approaches. We used a novel approach of online two-dimensional liquid chromatography-tandem MS with high-resolution reversed-phase liquid chromatography (RPLC), alternating electron transfer dissociation (ETD) and collision-induced dissociation (CID) on precursor ions to maximize fragmentation of uniquely modified species. The first online RPLC separation sorted histone families, then RPLC or weak cation exchange hydrophilic interaction liquid chromatography (WCX-HILIC) separated species heavily clad in PTMs. Tentative identifications were assigned by matching proteoform masses to predicted theoretical masses that were verified with tandem MS. We used this innovative approach for histone-intact protein PTM mapping (HiPTMap) to identify and quantify proteoforms purified from CD8 T cells after in vivo influenza infection. Activation significantly altered PTMs following influenza infection, histone maps changed as T cells migrated to the site of infection, and T cells responding to secondary infections had significantly more transcription enhancing modifications. Thus, HiPTMap identified and quantified proteoforms and determined changes in CD8 T cell histone PTMs over the course of infection.

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Section: Discussionmentioning

confidence: 79%

Mapping Influenza-Induced Posttranslational Modifications on Histones from CD8+ T Cells

Rezinciuc

Tian

et al. 2020

Viruses

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“…Likewise, epigenetic changes by influenza may alter the host response. Alterations in DNA methylation reportedly occur in promoter regions of some proinflammatory cytokines and interleukin genes in response to influenza infection [114][115][116]. Influenza infection can also modulate PTMs [117,118].…”

Section: Discussionmentioning

confidence: 99%

Mapping Influenza-Induced Posttranslational Modifications on Histones from CD8+ T Cells

Rezinciuc¹,

Wu²,

Hengel³

et al. 2020

Preprint

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T cell function is determined by transcriptional networks that are regulated by epigenetic programming via posttranslational modifications (PTMs) of histone proteins and DNA. Bottom-up mass spectrometry (MS) can identify histone PTMs, whereas intact protein analysis with high-field Fourier transform ion cyclotron resonance MS (FTICR-MS) can detect species missed by bottom-up approaches. We used high-resolution reversed-phase liquid chromatography (RPLC) FTICR-MS, alternating electron transfer dissociation (ETD) and collision-induced dissociation (CID) on precursor ions to maximize fragmentation of uniquely modified species. First online RPLC separation sorted histone families then weak cation exchange hydrophilic interaction liquid chromatography (WCX-HILIC) separated species heavily clad in PTMs. Tentative PTM identifications were assigned by matching peptide masses to predicted theoretical masses that were verified with tandem MS. We used this innovative approach for Histone-intact protein PTM mapping (HiPTMap) and to quantify PTMs on core histones purified from CD8+ T cells directly isolated ex vivo post-influenza infection. Activation significantly reduced PTMs in vivo following influenza infection, histone maps changed as T cells migrated to infections, and T cells responding to secondary heterologous infections had significantly more PTMs enhancing transcriptional activation. Thus, HiPTMap identifies and quantifies PTMs on CD8+ T cell histones and determines their combinations in T cell states.

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“…In the development of Epstein–Barr-virus-associated gastric carcinoma, DNA hypermethylation was observed [ 36 ]. The total DNA methylation level was significantly up-regulated in the thymus and bursa of chicken after H5N1 influenza viral infection (A-H5N1 strain) [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Global and Complement Gene-Specific DNA Methylation in Grass Carp after Grass Carp Reovirus (GCRV) Infection

Xiong

Luo

et al. 2018

IJMS

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Grass carp reovirus (GCRV) causes huge economic loss to the grass carp cultivation industry but the mechanism remains largely unknown. In this study, we investigated the global and complement gene-specific DNA methylation in grass carp after GCRV infection aimed to uncover the mechanism underlying GCRV infection. The global DNA methylation level was increased after GCRV infection. Expression levels of enzymes involved in DNA methylation including DNA methyltransferase (DNMT), ten-eleven translocation proteins (TETs), and glycine N-methyltransferase (GNMT) were significantly altered after GCRV infection. In order to investigate the relationship between the gene expression level and DNA methylation level, two representative complement genes, complement component 3 (C3) and kininogen-1 (KNG1), were selected for further analysis. mRNA expression levels of the two genes were significantly increased at 5 and 7 days after GCRV infection, whereas the DNA methylation level at the 5′ flanking regions of the two genes were down-regulated at the same time-points. Moreover, a negative correlation was detected between gene expression levels and DNA methylation levels of the two genes. Therefore, the current data revealed a global and complement gene-specific DNA methylation profile after GCRV infection. Our study would provide new insights into understanding the mechanism underlying GCRV infection.

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Changes in methylation of genomic DNA from chicken immune organs in response to H5N1 influenza virus infection

Cited by 16 publications

References 37 publications

Mapping Influenza-Induced Posttranslational Modifications on Histones from CD8+ T Cells

Mapping Influenza-Induced Posttranslational Modifications on Histones from CD8+ T Cells

Mapping Influenza-Induced Posttranslational Modifications on Histones from CD8+ T Cells

Global and Complement Gene-Specific DNA Methylation in Grass Carp after Grass Carp Reovirus (GCRV) Infection

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