Changes in Microbial Diversity, Methanogenesis and Fermentation Characteristics in the Rumen in Response to Medicinal Plant Extracts

Abstract

This study evaluated the in vitro effect of medicinal plant extracts on ruminal methanogenesis, four different groups of methanogens and ruminal fermentation characteristics. A fistulated Holstein cow was used as a donor of rumen fluid. Licorice and mugwort extracts (Glycyrrhiza uralensis and Artemisia capillaris, 0.5% and 1% of total substrate DM, respectively), previously used as folk remedies, were added to an in vitro fermentation incubated with buffered-rumen fluid. Total gas production in Glycyrrhiza uralensis extract treatment was not significantly different between treatments (p<0.05) while total gas production in the Artemisia capillaris extract treatment was lower than that of the control. Artemisia capillaris extract and Glycyrrhiza uralensis extract reduced CH4 emission by 14% (p<0.05) and 8% (p<0.05), respectively. Ciliate-associated methanogens population decreased by 18% in the medicinal plant extracts treatments. Medicinal plant extracts also affected the order Methanobacteriales community. Methanobacteriales diversity decreased by 35% in the Glycyrrhiza uralensis extract treatment and 30% in the Artemisia capillaris extract treatment. The order Methanomicrobiales population decreased by 50% in the 0.5% of Glycyrrhiza uralensis extract treatment. These findings demonstrate that medicinal plant extracts have the potential to inhibit in vitro ruminal methanogenesis.

Evaluation of nutritional and economic feed values of spent coffee grounds andArtemisia princepsresidues as a ruminant feed usingin vitroruminal fermentation

“…These by-products were chosen primarily because of the rapid increase in their production, and their relatively high content of nutrients and bio-active compounds ( Wallace, 2007 ; Acevedo et al, 2013 ). It was expected that the bio-active phenolic compounds contained in these by-products would modulate rumen microbial activity, fermentation characteristics, and CH 4 emission ( Acevedo et al, 2013 ; Kim et al, 2013 ). Fermentation of selected by-products using Lactobacillus spp.…”

МЕТАБОЛИЧЕСКИЕ ПАРАМЕТРЫ И ИНТЕНСИВНОСТЬ МЕТАНООБРАЗОВАНИЯ В РУБЦОВОЙ ЖИДКОСТИ in vitro ПРИ ТЕСТИРОВАНИИ РАЦИОНОВ С ДОБАВКАМИ РАСТИТЕЛЬНЫХ ПРЕПАРАТОВ И CoCl2

“…Возможно, снижение метаногенеза происходит и в результате изменения в популяциях метаногенных бактерий в рубцовой жидкости. Ранее обнаружено, что экстракт A. capillaris уменьшал выделение метана на 14 % (р < 0,05) после 48 ч инкубации при снижении численности сообществ метаногенов (инфузории и популяции метанобактерий) (53). Аналогичный эффект наблюдался под влиянием смеси эфирных масел и карбоната кобальта (16).…”