Changes in microbiological metabolism under chemical stress

Ray, Sylvian R.; Peters, Catherine A.

doi:10.1016/j.chemosphere.2007.10.026

Cited by 35 publications

(30 citation statements)

References 31 publications

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“…It acts directly on metabolism by inducing cellular oxidation and inhibiting phosphorylation, leading to the uncoupling of oxidative phosphorylation (Ray and Peters, 2008). Therefore, the cellular energy in the mitochondria is released directly as heat due to this uncoupling and results in uncontrolled thermogenesis.…”

Section: Discussionmentioning

confidence: 99%

Clinical features and treatment in patients with acute 2,4-dinitrophenol poisoning

Jiang

Huang

2011

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To report clinical features and treatment of 16 cases of acute 2,4-dinitrophenol poisoning. Methods: A total of 16 patients suffering from acute poisoning due to non-oral exposure to 2,4-dinitrophenol were sent to our hospital. Two died within 3 h after admission, while the other 14 responded to supportive treatment and hemoperfusion. Clinical features and treatment of the patients were retrospectively analyzed and presented. Results: Fourteen patients recovered and were discharged after four to six weeks of treatment. No obvious poisoning sequelae were found in the three-month follow-up. Conclusions: Non-oral exposure to 2,4-dinitrophenol is toxic. Hemoperfusion and glucocorticoid treatments may be efficient measures to prevent mortality, but this requires further study.

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Section: Discussionmentioning

confidence: 99%

Clinical features and treatment in patients with acute 2,4-dinitrophenol poisoning

Jiang

Huang

2011

J. Zhejiang Univ. Sci. B

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“…The genes needed for alkane oxidation and ␤-oxidation are strongly upregulated in cells grown on pyruvate but were also upregulated as an immediate response in hexadecane-grown Alcanivorax cells. Energetic processes involved in solvent tolerance in Pseudomonas putida have been recently reported (39,61). A proteomic study of P. putida demonstrated that under toluene stress, the energy supply was heavily affected as a result of the disruption of the proton motive force.…”

Section: Discussionmentioning

confidence: 99%

Adaptation of the Hydrocarbonoclastic Bacterium Alcanivorax borkumensis SK2 to Alkanes and Toxic Organic Compounds: a Physiological and Transcriptomic Approach

Naether

Slawtschew

Stasik

et al. 2013

Appl Environ Microbiol

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The marine hydrocarbonoclastic bacterium Alcanivorax borkumensis is able to degrade mixtures of n-alkanes as they occur in marine oil spills. However, investigations of growth behavior and physiology of these bacteria when cultivated with n-alkanes of different chain lengths (C 6 to C 30 ) as the substrates are still lacking. Growth rates increased with increasing alkane chain length up to a maximum between C 12 and C 19 , with no evident difference between even-and odd-numbered chain lengths, before decreasing with chain lengths greater than C 19 . Surface hydrophobicity of alkane-grown cells, assessed by determination of the water contact angles, showed a similar pattern, with maximum values associated with growth rates on alkanes with chain lengths between C 11 and C 19 and significantly lower values for cells grown on pyruvate. A. borkumensis was found to incorporate and modify the fatty acid intermediates generated by the corresponding n-alkane degradation pathway. Cells grown on distinct n-alkanes proved that A. borkumensis is able to not only incorporate but also modify fatty acid intermediates derived from the alkane degradation pathway. Comparing cells grown on pyruvate with those cultivated on hexadecane in terms of their tolerance toward two groups of toxic organic compounds, chlorophenols and alkanols, representing intensely studied organic compounds, revealed similar tolerances toward chlorophenols, whereas the toxicities of different n-alkanols were significantly reduced when hexadecane was used as a carbon source. As one adaptive mechanism of A. borkumensis to these toxic organic solvents, the activity of cis-trans isomerization of unsaturated fatty acids was proven. These findings could be verified by a detailed transcriptomic comparison between cultures grown on hexadecane and pyruvate and including solvent stress caused by the addition of 1-octanol as the most toxic intermediate of n-alkane degradation.

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“…In a previous study, we used respirometry to study the impact of stressors 2,4-dinitrophenol, (DNP), pentachlorophenol (PCP), and N-ethyl maleimide (NEM) on the metabolism of Pseudomonas aeruginosa [21]. We measured biological activity simultaneously in stressed and nonstressed systems to infer the effect of the chemical stressors and found that at low concentrations these chemical stressors affected metabolism by reducing biomass yields but not diminishing substrate utilization.…”

Section: Introductionmentioning

confidence: 99%

Adaptations in microbiological populations exposed to dinitrophenol and other chemical stressors

Ray

Peters

2010

Enviro Toxic and Chemistry

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Microbiological populations in natural and engineered systems may experience multiple exposures to chemical stressors, which may affect system functions. The impact of such exposures on the metabolism of a population of Pseudomonas aeruginosa was studied using respirometry. Two serial exposures to low concentrations of 2,4-dinitrophenol (DNP), pentachlorophenol (PCP), or N-ethyl maleimide (NEM) did not affect metabolism beyond that expected for a single exposure. However, at higher concentrations, three exposures to DNP led to a combination of metabolic stress and resilience in the population. At a low DNP concentration of 400 mg/L, multiple exposures led to increased stress but indicated no development of resilience. At a high DNP concentration of 1,200 mg/L, no biological activity was observed, indicating that the population did not survive the exposure. At intermediate concentrations of 800 and 900 mg/L DNP, stress was observed, but it was found to decrease after multiple exposures. This, combined with the observation that the size of the population decreased, indicated that resilience in the population had developed because of elimination of the weaker organisms in the population. In contrast, the lack of resilience at the lower DNP concentration was attributed to the survival of the strong as well as weak members, lowering the resilience of the population as a whole. The development of resilience within a window of stressor concentrations is an important finding with implications for predicting the performance of biotreatment processes and biosensor technologies and for interpreting ecotoxicity risk assessments.

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Changes in microbiological metabolism under chemical stress

Cited by 35 publications

References 31 publications

Clinical features and treatment in patients with acute 2,4-dinitrophenol poisoning

Clinical features and treatment in patients with acute 2,4-dinitrophenol poisoning

Adaptation of the Hydrocarbonoclastic Bacterium Alcanivorax borkumensis SK2 to Alkanes and Toxic Organic Compounds: a Physiological and Transcriptomic Approach

Adaptations in microbiological populations exposed to dinitrophenol and other chemical stressors

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