Changes in Microglial Inflammation-Related and Brain-Enriched MicroRNAs Expressions in Response to In Vitro Oxygen–Glucose Deprivation

Kong, Huimin; Omran, Ahmed; Ashhab, Muhammad Usman; Peng, Jing; He, Fang; Wu, Liwen; Deng, Xingming; Yin, Fei

doi:10.1007/s11064-013-1209-7

Cited by 23 publications

(18 citation statements)

References 62 publications

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“…Significant upregulation of miR‐124 was found in oxygen–glucose deprivation model (Kong et al. 2014). In our study, we did not detect a statistically significant change in the expression of miR‐124 (Fig.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA regulation in an animal model of acute ocular hypertension

Wang

Valiente‐Soriano

Nadal-Nicolás

et al. 2016

Acta Ophthalmologica

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PurposeTo analyse miRNA regulation in a rat model of acute ocular hypertension (AOH).MethodsAcute ocular hypertension (AOH) was induced in the left eye of adult albino rats by inserting a cannula connected with a saline container into the anterior chamber. The contralateral eye served as a control. Seven days later, animals were killed. Retinas were used either for quantitative analysis of retinal ganglion cells (RGCs) and microglial cells or for miRNA array hybridization, qRT‐PCR and Western blotting.ResultsAnatomically, AOH caused axonal degeneration, a significant loss of RGCs and a significant increase in microglial cells in the ganglion cell layer. The miRNAs microarray analysis revealed 31 differentially expressed miRNAs in the AOH versus control group, and the regulation of 12 selected microRNAs was further confirmed by qRT‐PCR. Bioinformatic analysis indicates that several signalling pathways are putatively regulated by the validated miRNAs. Of particular interest was the inflammatory pathway signalled by mitogen‐activated protein kinases (MAPKs). In agreement with the in silico analysis, p38 MAP kinase, tumour necrosis factor‐alpha (TNF‐α) and iNOS proteins were significantly upregulated in the AOH retinas.ConclusionsAcute IOP elevation led to changes in the expression of miRNAs, whose target genes were associated with the regulation of microglia‐mediated neuroinflammation or neural apoptosis. Addressing miRNAs in the process of retinal ischaemia and optic nerve damage in association with high IOP elevation may open new avenues in preventing retinal ganglion cell apoptosis and may serve as target for future therapeutic regimen in acute ocular hypertension and retinal ischaemic conditions.

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Section: Discussionmentioning

confidence: 99%

MicroRNA regulation in an animal model of acute ocular hypertension

Wang

Valiente‐Soriano

Nadal-Nicolás

et al. 2016

Acta Ophthalmologica

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“…In our previous work we found significant upregulation in these miRNAs expressions in the astrocytes stimulated with lipopolysaccharides (LPS) [28] and also in microglia exposed to oxygen glucose deprivation (OGD) [29]. Further experiments will need to prove if these miRNAs have a proinflammatory function or not in this animal model.…”

Section: Discussionmentioning

confidence: 92%

MicroRNAs expression changes in acute Streptococcus pneumoniae meningitis

Omran¹,

Jagoo

Ashhab

et al. 2014

Translational Neuroscience

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Background: Despite prompt and appropriate care, Streptococcus pneumoniae (S. pneumoniae) meningitis remains a key cause of childhood morbidity and mortality. Recently, several studies suggested the possibility of microRNAs (miRNA) involvement in multiple brain and infectious diseases. This study aimed to investigate the expressional changes of brain-enriched miRNAs (124, 134, 9, and 138), and inflammation-related miRNAs (132, 181a, 221, and 222) in the cerebral cortex of acute S. pneumoniae meningitis in postnatal day 25 rats with and without treatment. Methodology: Quantitative polymerase chain reaction (qPCR) was used to measure the expression levels of the tested brain-enriched and inflammation-related miRNAs in the cerebral cortical tissues obtained from acute experimental meningitis rat model induced by intracranial inoculation of S. pneumoniae serotype 3 with and without treatment. Control rats inoculated with saline were also included. Results: Brainenriched miRNAs are significantly downregulated in untreated animals while after treatment with antibiotic and steroid for 24 hours, miR-124 and miR-9 expressions were nearly equal to the control, while miR-134 and miR-138 were significantly upregulated. Inflammation-related miR-132 was significantly downregulated in untreated and treated animals while miRNAs (181a, 221, and 222) were significantly upregulated in untreated and treated animals. Conclusion: Acute S. pneumoniae meningitis leads to significant changes in the cerebral cortical expressions of some brain-enriched and inflammation-related miRNAs. These miRNAs may play a role in the pathogenesis of acute bacterial meningitis.

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“…To date, our group has demonstrated the differential expressions of inflammation-related and brain-enriched miRNAs in activated glias [22,30] and MTLE rat model [14] . Next we have planned to modulate the expressions of some miRNAs in rat MTLE and meningitis models to discuss its potential as a therapeutic target for CNS diseases.…”

Section: Research Highlightmentioning

confidence: 99%

“…In our recent study entitled "Changes in Microglial Inflammation-related and Brain-enriched MiRNAs Expression in Response to In vitro Oxygen-Glucose Deprivation" [22] we used primary rat microglia cells OGD activated model and detected the expressions of the above 10 inflammation-related and brain-enriched miRNAs at different activated conditions. We found that the viability of the microglia was timedependent after OGD pretreatment.…”

Section: Research Highlightmentioning

confidence: 99%

“…Our research group is vigorously working to understand the mechanisms of many CNS diseases including meningitis [25][26][27] , intractable epilepsy [13,15,28] , and ischemic injuries [22] . Recent studies have shown that brain inflammation is an important factor contributing to almost all CNS diseases and activation of microglia is considered to be neuroinflammation hallmark.…”

Section: Research Highlightmentioning

confidence: 99%

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Inflammation-related and Brain-enriched MicroRNAs Influence the Activation of Microglia Response to In vitro Oxygen-Glucose Deprivation

Kong¹,

Omran²,

Aly³

et al. 2014

Inflamm Cell Signal

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Microglia is one of the major resident immunecompetent cells in the central nervous system (CNS) and has become an important cellular component for understanding brain diseases. MicroRNAs (miRNAs) are small, noncoding RNAs molecules that regulate expression of protein-coding mRNAs on the post-transcriptional level; miRNAs plays important roles in microglial activation in response to brain ischemia and other stressors. Through culturing primary rat microglial cells and establishing a microglial activation model by oxygenglucose deprivation (OGD). We found that the viability of the microglia was time-dependent and expressions of inflammation-related miRNAs (miR-146a, -21, -181a, -221, and -222), and brain-enriched miRNAs (miR-124, -134, -9, -132, and -138) in microglia were modulated in an OGD model of ischemic insult. This research highlight discusses the findings of the recent study and the investigators' active research endeavors.

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Changes in Microglial Inflammation-Related and Brain-Enriched MicroRNAs Expressions in Response to In Vitro Oxygen–Glucose Deprivation

Cited by 23 publications

References 62 publications

MicroRNA regulation in an animal model of acute ocular hypertension

MicroRNA regulation in an animal model of acute ocular hypertension

MicroRNAs expression changes in acute Streptococcus pneumoniae meningitis

Inflammation-related and Brain-enriched MicroRNAs Influence the Activation of Microglia Response to In vitro Oxygen-Glucose Deprivation

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