Changes in microRNA expression in rat lungs caused by sevoflurane anesthesia: a TaqMan^|^reg; low-density array study

Tanaka, Shunsuke; Ishikawa, Masashi; Arai, Masae; Genda, Yuuki; Sakamoto, Atsuhiro

doi:10.2220/biomedres.33.255

Cited by 25 publications

(20 citation statements)

References 34 publications

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“…The two drugs have been used extensively in previous studies concerning the effects of anesthetic agents on gene expression (7-9), protein expression (10) and metabolites (11) in the rat brain. Changes in the miRNA expression levels in the hippocampi of rats anesthetized with one minimum alveolar concentration of inhaled anesthetics (sevoflurane at 2.4%; 22) or with continuous infusion of intravenous anesthetics (propofol at 600 µg/kg/min; 23) were investigated in the present study. Infusion of propofol at 600 µg/kg/min is the median effective dose for rats (26).…”

Section: Discussionmentioning

confidence: 99%

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Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia

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Hori

Ishikawa

et al. 2014

Molecular Medicine Reports

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Abstract. General anesthesia is commonly used in the surgical arena, but little is known regarding its influence at the genomic and molecular levels. MicroRNAs (miRNAs) belong to a new class of non-coding RNA molecules which influence cell biology. In the present study, it was hypothesized that miRNAs alter gene expression levels under general anesthesia. The aim was to compare the miRNA expression profiles in the rat hippocampus in response to anesthesia with representative volatile (sevoflurane) and intravenous (propofol) anesthetics. Wistar Rats were randomly assigned to either a 2.4% sevoflurane, 600 µg/kg/min propofol or control (without anesthetics) group. Total RNA from hippocampal samples which contained miRNA was subjected to quantitative reverse transcriptionpolymerase chain reaction and Taqman Low-Density Arrays (TLDA). A total of 373 miRNAs are associated with rats and the TLDA analysis revealed that 279 expressed miRNAs (74.8%) were expressed in all three groups. Significant differences in the levels of 33 of the 279 expressed miRNAs (11.8%) were observed among the three groups in response to the anesthetic agents, and when compared with the control group, significant differences were found in 26 of the 279 expressed miRNAs (9.3%). Following sevoflurane anesthesia, the levels of four miRNAs were significantly increased and those of 12 were significantly reduced. By contrast, following propofol anesthesia, the levels of 11 miRNAs were significantly reduced but no miRNAs exhibited significantly elevated levels. Fourteen miRNAs were significantly differentially expressed between the two anesthesia groups. In conclusion, sevoflurane and propofol exerted different effects on miRNA expression in the rat hippocampus.

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Section: Discussionmentioning

confidence: 99%

“…Infusion of propofol at 600 µg/kg/min is the median effective dose for rats (26). The two doses were selected since lower doses may not induce adequate anesthesia and higher doses may produce hemodynamic changes (10,22) that may independently alter hippocampal miRNA expression levels.…”

Section: Discussionmentioning

confidence: 99%

Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia

Goto

Hori

Ishikawa

et al. 2014

Molecular Medicine Reports

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“…In our previous study, it was found that sevoflurane affects the expression of miRNAs in normal rat lung (6). However, such effects of anesthetic ALI remain unknown.…”

Section: Introductionmentioning

confidence: 95%

Volatile anesthetic sevoflurane ameliorates endotoxin-induced acute lung injury via microRNA modulation in rats

et al. 2015

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Abstract. Volatile anesthetics have a lung protective effect in acute lung injury (ALI). Our previous study showed sevoflurane affects the expression of microRNA (miRNA) that control various physiological systems by regulating messenger RNA (mRNA) expression. However, the association between the anti-inflammatory effect of sevoflurane and miRNAs modulation remains unknown. The aim of the present study was to investigate the effect of sevoflurane and the expression of miRNAs in an endotoxin-induced ALI model in rats. Wistar rats were randomly assigned to three groups [lipopolysaccharide (LPS), LPS-sevoflurane and control; n=8/group]. All the rats were mechanically ventilated and intravenously-administered LPS (saline as control). Two hours post-injury, general anaesthesia was performed for 4 h with 2% sevoflurane (LPS-sevoflurane). The LPS and the control groups did not receive anaesthesia. The severity of ALI was evaluated by partial pressure of oxygen/fraction of inspired oxygen and the mRNA expression of inflammatory cytokine. The miRNA expression in lung tissue was analyzed by a reverse transcription-quantitative polymerase chain reaction. LPS caused ALI, evidenced by the impairment of pulmonary function and increased mRNA levels of tumor necrosis factor-α, interleukin-6 and nuclear factor-κB. Sevoflurane improved pulmonary function and inhibited the increased mRNAs. Of the 219 miRNAs detected, 15 and nine miRNAs were significantly changed in the LPS and LPS-sevoflurane group, respectively. In the LPS-sevoflurane group, the expression of several miRNAs that regulate inflammation was significantly changed compared to the LPS group. In conclusion, the present data showed that sevoflurane influences the expression of the miRNAs that regulate inflammation. This result suggests that the changes in miRNA expression are involved in the lung protective mechanisms of volatile anesthetics. IntroductionAcute lung injury (ALI) is a major complication in critically ill patients (1). Therefore, it is important to preserve lung function and prevent further exacerbation for those patients whose lungs are already vulnerable prior to surgery. The volatile anesthetic sevoflurane is known to have organ protective effects in several pathological conditions. For example, Voigtsberger et al (2) have recently shown that sevoflurane ameliorates gas exchange and attenuates lung damage in a model of lipopolysaccharide (LPS)-induced ALI.MicroRNA (miRNA) is a newly discovered and non-coding RNA that regulates genome expression at the post-transcriptional level (3). miRNA is estimated to control 30-90% of human genes (4). miRNA has been suggested to either suppress messenger RNA (mRNA) translation or reduce mRNA stability by binding particular regions of mRNA (5). Recent published data have also revealed that the expression and function of miRNA are associated with a broad range of diseases.In our previous study, it was found that sevoflurane affects the expression of miRNAs in normal rat lung (6). However, such effects of anesthetic ALI ...

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“…On the second day, context testing was conducted 21 h after conditioning in the same chamber. Cued testing with altered context was conducted 26 h after conditioning using a triangular box (28.5 (L) × 32.5 (W) × 32.5 (H) cm) made influence on the expression of miRNAs in major organs such as the liver, lung, and brain of adult rats (5,9,28). Conversely, certain brain-specific miRNA regulates the size of dendritic spines, which are postsynaptic sites of excitatory synaptic transmission of rat hippocampal neurons (24).…”

Section: Contextual and Cued Fear Conditioning Testmentioning

confidence: 99%

<b>Influence of neonatal sevoflurane exposure on nerve development-related microRNAs and behavior of </b><b>rats </b>

et al. 2015

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Commonly used anesthetics adversely affect the developing brain, but the mechanisms remain unknown. We previously showed that the expressions of microRNAs (miRNAs) in major organs are affected by anesthetics. Therefore, we used TaqMan low-density array (TLDA) to analyze gene expression in the hippocampus of neonatal rats exposed to sevoflurane and performed behavioral tests after they reached adulthood to evaluate cognitive and memory function. Rat male pups at postnatal day 7 were exposed to 1.9% sevoflurane for 3 h, and the hippocampus-miRNA expression profile on postnatal day 8 was determined. Open field and fear conditioning tests conducted during postnatal weeks 7 and 8 indicated that sevoflurane-exposed rats, but not controls, exhibited anxiety-like disorders. TLDA analysis identified 20 differentially expressed miRNAs, which were not shared between postnatally and maturely sevoflurane-exposed rats. The level of rno-miR-632, which targets brain-derived neurotrophic factor and calcium channel, voltage-dependent, alpha 2/delta subunit 2, increased by 10-fold, indicating that exposure to sevoflurane during early neural development alters hippocampus-miRNA expression and may induce subsequent behavioral disorders.

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Changes in microRNA expression in rat lungs caused by sevoflurane anesthesia: a TaqMan^|^reg; low-density array study

Cited by 25 publications

References 34 publications

Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia

Changes in the gene expression levels of microRNAs in the rat hippocampus by sevoflurane and propofol anesthesia

Volatile anesthetic sevoflurane ameliorates endotoxin-induced acute lung injury via microRNA modulation in rats

<b>Influence of neonatal sevoflurane exposure on nerve development-related microRNAs and behavior of </b><b>rats </b>

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