Changes in Milk Composition as Affected by Subclinical Mastitis in Goats

Leitner, Gabriel; Merin, Uzi; Silanikove, Nissim

doi:10.3168/jds.s0022-0302(04)73325-1

Cited by 188 publications

(161 citation statements)

References 35 publications

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“…Our results are also consistent with previous studies showing that LPS challenge induces significant casein degradation and identifies milk PL as a major cause for this (Moussaoui et al, 2002;Leitner et al, 2004aLeitner et al, , 2004bLeitner et al, and 2004cSilanikove et al, 2006;Rossi et al, 2009). The biological implications of these findings have been discussed before Silanikove et al, 2006) and, therefore, these aspects are not discussed here in detail.…”

Section: Lps Challenge Is Reflected By Accumulation Of Inflammatory Isupporting

confidence: 93%

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Silanikove

Rauch-Cohen

Shapiro

et al. 2012

Animal

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The aim of this work was to study the effects of mastitis induced by intramammary lipopolysaccharide (LPS) challenge on milk oxidative stability, as well as to understand the underlying biochemical processes that cause such changes. LPS challenge was associated with nitric oxide burst from the surrounding mammary epithelial cells and consequently induced nitrosative stress that was induced by the formation of NO 2 d from nitrite by lactoperoxidase. This response was associated with an , 3-fold increased formation of hazardous compounds: nitrotyrosines, carbonyls and lipid peroxides. We sustained the involvement of xanthine oxidase as a major source of hydrogen peroxide. In consistent with previous findings, catalase has been shown to play a major role in modulating the nitrosative stress by oxidizing nitrite to nitrate. The current hygienic quality criteria cannot detect mixing of low-quality milk, such as milk with high somatic cells, and nitrite with high-quality milk. Thus, development of an improved quality control methodology may be important for the production of high-quality milk.Keywords: milk, cow, mastitis, NO and NO 2 d, plasmin system Implication In this study, we show that intramammary treatment with lipopolysaccharide induced nitric oxide burst that was associated with nitrosative stress that reflected the formation of hazardous compounds in milk: nitrotyrosines, carbonyls and lipid peroxides. These changes were associated with impairment in milk quality. Currently, very little is known about the presence of oxidative substances in commercial milk and their industrial and health implications. As mastitis is a prevalent problem in the dairy industry, this study stresses the importance of gaining further information on the effect of different forms of mastitis on milk composition.

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Section: Lps Challenge Is Reflected By Accumulation Of Inflammatory Isupporting

confidence: 93%

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Silanikove

Rauch-Cohen

Shapiro

et al. 2012

Animal

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“…Essas bactérias apresentam importância epidemiológica, pois podem causar mastite subclínica durante toda a lactação ou mesmo no período seco (Poutrel et al 1997), tornando os animais infectados fontes de infecção em potencial no rebanho. Some-se a isso a importância econômica desses agentes, que causam prejuízos aos produtores principalmente devido à redução na produção de leite (Leitner et al 2004).…”

Section: Resultsunclassified

Perfil microbiológico, celular e fatores de risco associados à mastite subclínica em cabras no semiárido da Paraíba

et al. 2010

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A subclinical mastitis study was conducted in nine dairy goat herds in the semi-arid region of Paraíba state, Northeastern Brazil, to determine the occurrence of infection, to evaluate microbiological and cellular profiles of the milk, to test the sensitivity of isolated microorganisms to antimicrobials, and to identify risk factors. One hundred thirty-one dairy goats were used, 261 samples were collected for microbiological culture and 131 samples for somatic cells count (SCC). During collection, the California Mastitis Test (CMT) was conducted and an epidemiological questionnaire was applied for each herd. There was bacterial growth in 30 samples (11.49%), with 25 (83.33%) coagulase-negative Staphylococcus and five (16.66%) Staphylococcus aureus isolated. The SCC mean was 1.39x10 6 cells/ml. CMT presented low sensitivity (46.7%) and low specificity (60.6%) compared with microbiological culture. Gentamicin and the association of neomycin, bacitracin and tetracyclin were the antimicrobials against which the microorganisms isolated showed 100% sensitivity. Penicillin and ampicillin had the greatest resistance rates (66.67% and 63.89%, respectively). Goat breeding is not the main activity on the farms and do not isolate diseased animals were identified as risk factors for caprine subclinical mastitis. Program for the control and prophylaxis of mastitis must be implemented focusing primarily on hygiene cares at milking and correction of the risk factors identified in this study.

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“…Avaliando a composição do leite de cabras com mastite subclínica, Leitner et al (2004), observaram resultados semelhantes aos observados para as ovelhas com mastite subclínica neste estudo, em que as concentrações de cálcio não diferiram entre os grupos, porém a sua concentração foi inferior no leite das cabras infectadas.…”

Section: Soro Lácteounclassified

Metabolismo energético, proteico e mineral de ovelhas Santa Inês hígidas e com mastite subclínica

et al. 2013

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RESUMO.-Este estudo teve por objetivo avaliar o metabolismo energético, proteico e mineral de ovelhas Santa Inês hígidas e com mastite subclínica acompanhadas durante o final da gestação e na lactação. Foram acompanhadas ovelhas submetidas ao mesmo sistema de criação semi-intensivo. Os animais foram avaliados conforme os momentos a seguir: 10 dias que precedeu o parto (dap) e 15 dias pós parto (dpp), 30 dpp, 60 dpp e 90 dpp. Os metabólitos sanguíneos foram avaliados a partir do momento que antecedeu ao parto e os metabólitos no soro lácteo nos momentos The study aimed to evaluate the energy, protein and mineral metabolism in Santa Inês ewes, healthy and with subclinical mastitis, followed up during late gestation and lactation periods. Ewes subjected to the same semi-intensive nursing system were followed up. The animals were evaluated according to the following stages: 10 days before parturition (dbp) and 15 days postpartum (dpp), 30 dpp, 60 dpp, and 90 dpp. Blood metabolites were evaluated starting from the stage previous to parturition and whey metabolites were evaluated in the subsequent stages. A screening of the ewes followed up in this study (12 healthy and 18 with subclinical mastitis) was performed after a clinical and bacteriological examination. During lactation, maintaining the same screening criteria, 11 healthy and 20 infected mammary glands were selected; the milk for whey extraction was collected from these glands. Energy profile metabolites (non-esterified fatty acids [NEFAs], ß-hydroxybutyrate [BHB], fructosamine, cholesterol and triglycerides), protein profile (total protein, albumin, urea and creatinine) and mineral profile (iron, copper, zinc, magnesium, total calcium, ionized calcium, sodium, and potassium) were measured in the blood serum. Calcium, sodium and potassium ions, as well as NEFAs and BHB were measured in the whey. Blood biochemistry revealed an influence (P<0.05) of the peripartum and lactation periods on the blood concentrations of NEFAs, BHB, cholesterol, albumin, urea, ionized calcium. An analysis of the whey also revealed an influence on the potassium ion. Ewes with subclinical mastitis showed higher (P<0.05) blood levels of cholesterol, albumin and copper; higher sodium ion concentrations and NEFAs, and lower potassium ion in whey. Good physical score of ewes observed during this study, combined with the biochemical findings, allowed us to conclude that there was a larger energy requirement in the first month of lactation; however, this requirement was not enough to trigger any metabolic disorder or the emergence of ketonemia, and these discrete changes were more apparent in ewes with subclinical mastitis.INDEX TERMS: Metabolic profile, sheep, pregnant ewes, lactating, blood serum, infection of the mammary gland, mastitis, whey.

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Changes in Milk Composition as Affected by Subclinical Mastitis in Goats

Cited by 188 publications

References 35 publications

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability

Perfil microbiológico, celular e fatores de risco associados à mastite subclínica em cabras no semiárido da Paraíba

Metabolismo energético, proteico e mineral de ovelhas Santa Inês hígidas e com mastite subclínica

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