Changes in monoclonal HLA-DR antigen expression in acute organophosphorus pesticide-poisoned patients

Xia, Chenyun; Wang, Mi; Liang, Qi; Yun, Ling’An; Kang, Housheng; Liu, Fan; Wang, Dongsheng; Zhang, Jianan

doi:10.3892/etm.2013.1356

Cited by 7 publications

(1 citation statement)

References 19 publications

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“…HLA genes are involved in the overall immune response, but more interestingly, HLA-DRB5 methylation was recently shown to be associated with pathological Alzheimer’s Disease (Yu et al, 2015). In addition, HLA-DR antigens, as detected by monoclonal antibodies using flow cytometry on peripheral blood mononuclear cells, were lower in individuals with acute organophosphate pesticide poisoning (Xia et al, 2014). These data provide initial support for a link between pesticide exposure, DNA methylation, and Alzheimer’s Disease.…”

Section: Discussionmentioning

confidence: 99%

Changes in DNA methylation over the growing season differ between North Carolina farmworkers and non-farmworkers

Howard

Hsu

Chen

et al. 2016

Int Arch Occup Environ Health

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Purpose The occupational risk to farmworkers, particularly chronic exposure to pesticides, is an acknowledged environmental and work-related health problem. Epigenetics has recently been shown to contribute to a number of complex diseases and traits, including measures of cognitive function and preclinical neurodegenerative disease. We sought to determine if changes in DNA methylation existed between farmworker and non-farmworker populations, and to identify the genes most likely involved in those changes. Methods Eighty-three farmworkers and 60 non-farmworkers were selected from PACE4, a community-based, participatory research project comparing occupational exposures between immigrant Latino farmworker and non-farmworker manual workers. Measurements of DNA methylation were performed with the Infinium HumanMethylation450 BeadChip, at the beginning and end of the 2012 growing season. Bonferroni adjustment was used to identify significant findings (p=1.03 × 10−7, based on 485,000 tested methylation sites), although less stringent criteria (i.e., p≤1 × 10−6) were used to identify sites of interest. Expression quantitative trait locus (eQTL) databases were used to help identify the most likely functional genes for each associated methylation site. Results Methylation at 36 CpG sites, located in or near 72 genes, differed between the two groups (p≤1 × 10−6). The difference between the two groups was generally due to an increase in methylation in the farmworkers, and a slight decrease in methylation in the non-farmworkers. Enrichment was observed in several biological pathways, including those involved in the immune response, as well as Growth Hormone Signaling, Role of BRCA1 in DNA Damage Response, p70S6K signaling, and PI3K Signaling in B Lymphocytes. Conclusions We identified considerable changes in DNA methylation at 36 CpG sites over the growing season that differed between farmworkers and non-farmworkers. Dominant pathways included immune-related (HLA) processes, as well as a number of diverse biological systems. Further studies are necessary to determine which exposures or behaviors are responsible for the observed changes, and whether these changes eventually lead to disease related phenotypes in this population.

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Section: Discussionmentioning

confidence: 99%