Changes in morphology and metabolism enable Mn‐oxidizing bacteria from mid‐oceanic ridge environment to counter metal‐induced stress

Fernandes, Sheryl Oliveira; Prakash, L. Surya; Binish, M.B.; Krishnan, K. P.; Kurian, P. John

doi:10.1002/jobm.201700580

Cited by 11 publications

(8 citation statements)

References 48 publications

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“…Many metal elements (e.g., chromium (Cr) and manganese (Mn)) were reported to change bacterial morphology . For instance, high concentration of Mn 2+ and chromate caused fourfold to eightfold changes in cell size and surface roughness increase . Herbaspirillum sp.…”

Section: Discussionmentioning

confidence: 99%

Bacterial growth, morphology, and cell component changes in Herbaspirillum sp. WT00C exposed to high concentration of selenate

et al. 2020

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Selenium (Se) is a nonmetallic element of the chalcogens. It is primarily available in natural environments as selenate and selenite oxoanions. Although selenate/selenite reduction in many microbes is widely studied at low concentrations (<50 mM), the effects of high selenate stress on bacterial growth, morphology, and cell components have not yet been studied. In this study, the response of Herbaspirillum sp. WT00C to selenate stress at high concentration is investigated by microbiological and scanning electron microscopy (SEM) techniques as well as proteomic analysis. Bacterial growth was seriously inhibited under high selenate concentrations and its growth‐inhibitory phase was prolonged with the increase of selenate concentrations. More interestingly, this bacterium was able to recover its growth even if the selenate concentration was up to 400 mM. Its growth inhibition period shortened to 6 h when the bacterium growing in 200 mM selenate for 28 h was reinoculated to the Luria‐Bertani medium containing 200 mM selenate. The high concentration of selenate also induces marked changes in the cell dimension and surface roughness, as revealed by SEM, along with compositional changes in the cell wall shown by proteomic analysis. The bacterial growth inhibition results from the marked downregulation of the α‐subunit of DNA polymerase III and RNA helicase, whereas its growth recovery is related to its high antioxidative activities. More NADPH synthesis and the upregulation of thioredoxin reductase and GPx are beneficial for Herbaspirillum sp. WT00C to establish and maintain a balance between oxidant and antioxidant intracellular systems for defending selenate toxicity. This study is an important contribution to understanding why Herbaspirillum sp. WT00C survives in a high concentration of selenate and how the bacterial cells respond physiologically to selenate stress at high concentration.

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Section: Discussionmentioning

confidence: 99%

Bacterial growth, morphology, and cell component changes in Herbaspirillum sp. WT00C exposed to high concentration of selenate

et al. 2020

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“…Thus, it is probable that the anammox bacteria adapt to an iron-limited environment by increasing the anammoxosome volume ratio to be able to absorb more iron. This shapes adaptation mechanism is similar to that of the Mn-oxidizing bacteria Halomonas meridiana and Marinobacter algicola, which adapt to Mn(II))-induced stress by increasing the cell length and volume to achieve better Mn (II) oxidizing ability (21). This shapeshifting phenomenon indicates a sensitivity and dependence of anammox bacteria to iron.…”

Section: Discussionmentioning

confidence: 80%

“…Similar morphological changes were reported in Mn-oxidizing bacteria, i.e., Halomonas meridiana and Marinobacter algicola in response to Mn (II)-induced stress. The cell length of Halomonas meridiana and Marinobacter algicola increased significantly to improve the Mn (II) oxidizing ability (21). The cell morphology of bacteria can be adapted dynamically depending on environmental conditions, a requirement for optimal survival and growth (17).…”

Section: Introductionmentioning

confidence: 99%

Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteria

Peng

Guan

Liu

et al. 2019

Preprint

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AbstractAnaerobic ammonium-oxidizing (anammox) bacteria play a key role in the global nitrogen cycle and the treatment of nitrogenous wastewater. These functions are closely related to the unique biophysical structure of anammox bacteria. However, the research on the biophysical ultrastructure of intact anammox bacteria is lacking. In this study, in-situ three-dimensional nondestructive ultrastructure imaging of whole anammox cell was performed using synchrotron soft X-ray nano-computed tomography and the total variation-based simultaneous algebraic reconstruction technique (TV-SART). Statistical and quantitative analyses of the ultrastructures of intact anammox bacteria were performed. The linear absorption coefficient values of the ultrastructures of anammox bacteria were calculated and the asymmetric structure of the anammox bacteria was quantified. On this basis, the shape adaptation of the anammox bacteria responses to Fe2+ were explored, and the underlying regulation mechanism of Fe2+ on anammox bacteria was explored. Furthermore, a promising method to study the biophysical properties of cells in different environments and engineering processes was proposed.Graphical AbstractStatement of SignificanceAnaerobic ammonium-oxidizing (anammox) bacteria play key role in global nitrogen cycle, and this physiological function depends on the unique morphology of anammox bacteria. In this study, synchrotron soft-X ray imaging technique coupled with simultaneous algebraic reconstruction technique with total variation (SART-TV) algorithm were performed to quantify the three-dimensional ultrastructure of the whole anammox bacteria for the first time. On this basis, the shape adaptation and mechanism of the anammox bacteria responses to Fe2+ were explored and a promising method for detecting the physiological properties of anammox bacteria was proposed.

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“…(b) LAC values at iron concentrations of 0, 0.015 mM, 0.03 mM and 0.06 mM. tion mechanism is similar to that of the Mn-oxidizing bacteria Halomonas meridiana and Marinobacter algicola, which adapt to Mn(II)-induced stress by increasing the cell length and volume to achieve better Mn(II) oxidizing ability (Fernandes et al, 2018). Accordingly, the LAC value changed with the A/C volume ratio [ Fig.…”

Section: Figurementioning

confidence: 92%

“…Halomonas meridiana and Marinobacter algicola in response to Mn (II)-induced stress. Fernandes et al (2018) reported that the cell length of Halomonas meridiana and Marinobacter algicola increased significantly to improve the Mn (II) oxidizing ability. The cell morphology of bacteria can be adapted dynamically depending on environmental conditions, a requirement for optimal survival and growth (Woldemeskel & Goley, 2017).…”

Section: Introductionmentioning

confidence: 99%

Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteria

Peng

Guan

Liu

et al. 2020

J Synchrotron Radiat

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Anaerobic ammonium-oxidizing (anammox) bacteria play a key role in the global nitrogen cycle and in nitrogenous wastewater treatment. The anammox bacteria ultrastructure is unique and distinctly different from that of other prokaryotic cells. The morphological structure of an organism is related to its function; however, research on the ultrastructure of intact anammox bacteria is lacking. In this study, in situ three-dimensional nondestructive ultrastructure imaging of a whole anammox cell was performed using synchrotron soft X-ray tomography (SXT) and the total variation-based simultaneous algebraic reconstruction technique (TV-SART). Statistical and quantitative analyses of the intact anammox bacteria were performed. High soft X-ray absorption composition inside anammoxosome was detected and verified to be relevant to iron-binding protein. On this basis, the shape adaptation of the anammox bacteria response to iron was explored.

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Changes in morphology and metabolism enable Mn‐oxidizing bacteria from mid‐oceanic ridge environment to counter metal‐induced stress

Cited by 11 publications

References 48 publications

Bacterial growth, morphology, and cell component changes in Herbaspirillum sp. WT00C exposed to high concentration of selenate

Bacterial growth, morphology, and cell component changes in Herbaspirillum sp. WT00C exposed to high concentration of selenate

Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteria

Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteria

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