Changes in Nitric Oxide Production Levels and Expression of Nitric Oxide Synthase Isoforms in the Rat Uterus during Pregnancy

Suzuki, Takehito; Mori, Chiemi; Yoshikawa, Hiroshi; Miyazaki, Yoko; Kansaku, Norio; Tanaka, Kazuaki; Takizawa, Takeo

doi:10.1271/bbb.90222

Cited by 22 publications

(19 citation statements)

References 16 publications

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“…On the other hand, NOS2 activity increases throughout pregnancy that peaks around mid-gestation [22]. Suzuki et al proposed that NO plays a supportive role in promoting embryo survival [23]. Purcell et al suggested that after implantation, both NOS3 and NOS2 might play a role in tissue remodeling, immunosuppression, and vasoregulation [24].…”

Section: Roles Of No and Adma In Normal Pregnanciesmentioning

confidence: 99%

Roles of Nitric Oxide and Asymmetric Dimethylarginine in Pregnancy and Fetal Programming

Huang

Hsieh

Chang

et al. 2012

IJMS

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Nitric oxide (NO) regulates placental blood flow and actively participates in trophoblast invasion and placental development. Asymmetric dimethylarginine (ADMA) can inhibit NO synthase, which generates NO. ADMA has been associated with uterine artery flow disturbances such as preeclampsia. Substantial experimental evidence has reliably supported the hypothesis that an adverse in utero environment plays a role in postnatal physiological and pathophysiological programming. Growing evidence suggests that the placental nitrergic system is involved in epigenetic fetal programming. In this review, we discuss the roles of NO and ADMA in normal and compromised pregnancies as well as the link between placental insufficiency and epigenetic fetal programming.

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Section: Roles Of No and Adma In Normal Pregnanciesmentioning

confidence: 99%

Roles of Nitric Oxide and Asymmetric Dimethylarginine in Pregnancy and Fetal Programming

Huang

Hsieh

Chang

et al. 2012

IJMS

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“…Our interest that a stretch-activated potassium channel (K2P) may play a role in normal myometrial relaxation is peaked by the persua- The notion that NO is generated in myometrium and responsible for regulation of uterine quiescence has been controversial [19] and yet high levels of myometrial expression of nitric oxide synthase(s) correlated with gestation until the time of labor need not be the only source of NO to affect the myometrium. Indeed, recent studies have examined the presence of NO-synthase (NOS) in uterine tissues including myometrium [34] and actions of NO in reproductive tissues suggest its critical role in normal reproduction [35] . A source for myometrial NO may not come from smooth muscle generation directly, but rather from the ability of NO generated in the uterine membranes or the vasculature to bind free cysteine forming cysteine-NO (Cys-NO) or hemoglobin-NO and be transported to the muscle where it may be released as nitrosyl ion (NO + ) and bind to reduced glutathione (GSH) to generate GSNO [36] .…”

Section: No-mediated Relaxationmentioning

confidence: 99%

A role of stretch-activated potassium currents in the regulation of uterine smooth muscle contraction

Buxton

Heyman

et al. 2011

Acta Pharmacol Sin

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Rates of premature birth are alarming and threaten societies and healthcare systems worldwide. Premature labor results in premature birth in over 50% of cases. Preterm birth accounts for three-quarters of infant morbidity and mortality. Children that survive birth before 34 weeks gestation often face life-long disability. Current treatments for preterm labor are wanting. No treatment has been found to be generally effective and none are systematically evaluated beyond 48 h. New approaches to the treatment of preterm labor are desperately needed. Recent studies from our laboratory suggest that the uterine muscle is a unique compartment with regulation of uterine relaxation unlike that of other smooth muscles. Here we discuss recent evidence that the mechanically activated 2-pore potassium channel, TREK-1, may contribute to contraction-relaxation signaling in uterine smooth muscle and that TREK-1 gene variants associated with human labor and preterm labor may lead to a better understanding of preterm labor and its possible prevention.

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“…Second-harmonic generation has also been used to measure changes in collagen structure during gestation. 2 Other groups have applied optical methods, such as Fourier-transform infrared (FTIR), 39 resonance, 40 and light-scattering 26 spectroscopy to measure salivary changes and the presence of nitric oxide or fetal nucleated red blood cells, respectively. To our knowledge, this is the first report using Raman spectroscopy (RS), a sensitive optical method, to study biochemical changes in tissue during pregnancy.…”

Section: Introductionmentioning

confidence: 99%

Detecting Biochemical Changes in the Rodent Cervix During Pregnancy Using Raman Spectroscopy

et al. 2012

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The goal of this research is to determine whether Raman spectroscopy (RS), an optical method that probes the vibrational modes of tissue components, can be used in vivo to study changes in the mouse cervix during pregnancy. If successful, such a tool could be used to detect cervical changes due to pregnancy, both normal and abnormal, in animal models and humans. For this study, Raman spectra were acquired before, during and after a 19-day mouse gestational period. In some cases, after Raman data was obtained, cervices were excised for structural testing and histological staining for collagen and smooth muscle. Various peaks of the Raman spectra, such as the areas corresponding to fatty acid content and collagen organization, changed as the cervix became softer in preparation for labor and delivery. These findings correspond to the increase in compliance of the tissue and the collagen disorganization visualized with the histological staining. The results of this study suggest that non-invasive RS can be used to study cervical changes during pregnancy, labor and delivery and can possibly predict preterm delivery before overt clinical manifestations, potentially lead to more effective preventive and therapeutic interventions.

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Changes in Nitric Oxide Production Levels and Expression of Nitric Oxide Synthase Isoforms in the Rat Uterus during Pregnancy

Cited by 22 publications

References 16 publications

Roles of Nitric Oxide and Asymmetric Dimethylarginine in Pregnancy and Fetal Programming

Roles of Nitric Oxide and Asymmetric Dimethylarginine in Pregnancy and Fetal Programming

A role of stretch-activated potassium currents in the regulation of uterine smooth muscle contraction

Detecting Biochemical Changes in the Rodent Cervix During Pregnancy Using Raman Spectroscopy

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