Changes in NOTCH1 gene and its regulatory circRNA, hsa_circ_0005986 expression pattern in human gastric adenocarcinoma and human normal fibroblast cell line following the exposure to extremely low frequency magnetic field

Mansoury, Fereshteh; Babaei, Nahid; Abdi, Soheila; Entezari, Maliheh; Doosti, Abbas

doi:10.1080/15368378.2021.1891092

Cited by 6 publications

(5 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AGS cell lines were prepared from the Iranian Biological Resource Center. The cell culture method has been described in our previous report (20). All the cell lines were incubated at 37ºC in a 5% CO2 with the water-saturated condition.…”

Section: Cell Culturementioning

confidence: 99%

See 1 more Smart Citation

Extremely Low Frequency Electromagnetic Fields Alter Expression of C-Myc and circ-CCDC66 in Gastric Cancer Cell Line

et al. 2022

Self Cite

View full text Add to dashboard Cite

: The biological effects of electromagnetic fields (EMF), in particular their beneficial or adverse impacts on the promotion and progression of cancer, have attracted considerable attention. C-Myc has a critical regulatory role in cell transformation and causes metabolic changes, that accompany malignant transformation. Observations have shown that c-Myc expression can be altered by circular RNAs (circRNA). In this study, the changes in the expression of c-Myc and circ-CCDC66 in the AGS cell line after the extremely low-frequency magnetic fields (ELF-MF) exposure were investigated. The AGS cells were exposed to different magnetic flux densities for 18 h, continuously and discontinuously (2h on/2h off). To evaluate the expression changes of c-Myc and circ-CCDC66 real-time PCR was used. The results showed that discontinuous magnetic fields could reduce c-Myc expression. Continuous exposure of 0.25 mT could reduce the c-Myc expression, but with increasing the magnetic flux density c-Myc was upregulated. The expression level of circ-CCDC66 decreased under exposure to continuous and discontinuous ELF-MFs. The difference between the results of c-Myc under the influence of magnetic fields may be described by the hypothesis of the window effect of the fields. Our results indicated that ELF-MFs can induce changes in c-Myc and circ-CCDC66 expression.

show abstract

Section: Cell Culturementioning

confidence: 99%

“…To measure expression changes of c-Myc and circ-CCDC66 real-time PCR was used. RNA extraction, cDNA synthesis, and performing quantitative real-time polymerase chain reaction (qRT-PCR) were described totally in our previous report (20). The primer sequences are listed in Table 1.…”

Section: Quantitative Real-time Polymerase Chain Reactionmentioning

confidence: 99%

Extremely Low Frequency Electromagnetic Fields Alter Expression of C-Myc and circ-CCDC66 in Gastric Cancer Cell Line

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The primer sequences are listed in Table 1. The 2-∆∆CT method was applied to determine relative changes in gene expression in samples (26,29). Each experiment consisted of six separated flasks of cells.…”

Section: Quantitative Real-time Polymerase Chain Reaction (Qrt-pcr)mentioning

confidence: 99%

Pulsed Magnetic Fields Cause hes1 and hsa-Circ-0068530 Expression Changes in Gastric Cancer Cell Line and Human Normal Fibroblast Cell Line

Mansoury

Abdi

Babaei

et al. 2021

Jentashapir J Cell Mol Biol

Self Cite

View full text Add to dashboard Cite

Background: In recent years, the relationship between cancer cells and electromagnetic radiation has received much attention. Objectives: The present study aimed to evaluate the effects of different intensities of electromagnetic fields on gastric cancer cell lines (AGS). Methods: After preparing AGS and Hu02 (normal) cell lines, they were exposed to magnetic flux densities of 0.25, 0.5, 1, and 2 millitesla (mT) for 18 h. The cell viability was studied by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression levels of hes1 and hsa-circ-0068530 RNAs were studied by the quantitative Real-time-PCR technique. Results: The inhibition of gastric cancer cell line growth was observed under the influence of electromagnetic fields at different intensities. However, they did not affect the viability of normal cells. A sharp increase in the expression of hes1 and hsa-circ-0068530 genes was observed in normal cells exposed to 2 mT electromagnetic fields. Conclusions: In general, it can be concluded that the effect of electromagnetic fields on gastric cancer cells depends on their intensity. Magnetic flux densities of 0.25 and 0.5 mT had anti-cancer effects and magnetic flux density of 2 mT showed carcinogenic effects.

show abstract

“…In recent years, several in vitro studies exposing various cell lines to extremely lowfrequency electromagnetic fields (ELF-EMF) (3 Hz-3kHz) are being published in order to understand the mechanisms of interaction between biological systems and this type of nonionising radiation (Cios et al, 2021;Mansoury et al, 2021;Maleki et al, 2022;Mansoury OPEN ACCESS EDITED BY et al, 2022;Nieminen et al, 2022;Sołek et al, 2022;Elexpuru-Zabaleta et al, 2023;Lazzarini et al, 2023). Magnetic field exposure systems are designed to expose cell samples or devices to controlled magnetic fields and are widely used in scientific and industrial applications (Kirschvink, 1992).…”

Section: Introductionmentioning

confidence: 99%

“…Magnetic field exposure systems are designed to expose cell samples or devices to controlled magnetic fields and are widely used in scientific and industrial applications (Kirschvink, 1992). These systems can typically contain different coil geometries that act as passive elements for magnetic field generation such as solenoids (Han et al, 2018;Sanie-Jahromi and Saadat, 2018;Costantini et al, 2019;Samiei et al, 2020;Mansoury et al, 2021;Salek et al, 2021;Maleki et al, 2022;Mansoury et al, 2022), circular (Yuan et al, 2018;López et al, 2019;García-minguillán et al, 2020;Xu et al, 2020;Yuan et al, 2020;Asadian et al, 2021) or square (Consales et al, 2018;Sun et al, 2018;Zuo et al, 2020;Elexpuru-Zabaleta et al, 2023;Lazzarini et al, 2023) coils arranged in different configurations, such as the Merrit configuration (Kirschvink, 1992) or the Helmholtz configuration (Kirschvink, 1992;López et al, 2019;García-minguillán et al, 2020;Xu et al, 2020;Asadian et al, 2021;Cios et al, 2021;Lim et al, 2021). The latter is one of the most popular configurations for exposing cell cultures or small animals to ELF-EMF (López et al, 2019;Garcíaminguillán et al, 2020;Xu et al, 2020;Asadian et al, 2021;Cios et al, 2021;Lim et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Design and characterisation of a cell exposure system with high magnetic field homogeneity: RILZ coils

Rivera González,

López de Mingo,

Amuneke Ramírez

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

In vitro studies requiring controlled exposure to low-frequency electromagnetic fields employ exposure systems with different geometries and configurations, the Helmholtz configuration being one of the most widely used. This configuration has limitations in the homogeneity of the spatial distribution of the magnetic field intensity values. We present the design, manufacturing, and characterisation of a new coil system, called RILZ configuration, which improves the distribution of magnetic field intensity values in the three dimensions of space for three different heights in comparison with the traditional circular coils in Helmholtz configuration. In addition, a comparative study of the cellular response in CT2A cultures exposed to a magnetic field of 50 Hz and 100 µT for 48 hrs is performed with both exposure systems. The results of the study show reduced values of deviation from the central value of magnetic field intensity using the RILZ coil system. These differences are statistically significant compared to the Helmholtz configuration for the three Cartesian directions: x (p < 0.01), y (p < 0.01), z (p < 0.01). In addition, the intensity values for three different heights are statistically significantly correlated using the RILZ coil system (p < 0.01). The differences in cell behaviour are also statistically significant between the two systems (p < 0.01) and may be directly related to the differences found in the distribution of intensity values between the two systems. This study highlights the importance of the homogeneity of the magnetic field intensity generated by the exposure systems used and offers an effective solution to control the magnetic field exposure parameters in vitro assays.

show abstract

Changes in NOTCH1 gene and its regulatory circRNA, hsa_circ_0005986 expression pattern in human gastric adenocarcinoma and human normal fibroblast cell line following the exposure to extremely low frequency magnetic field

Cited by 6 publications

References 26 publications

Extremely Low Frequency Electromagnetic Fields Alter Expression of C-Myc and circ-CCDC66 in Gastric Cancer Cell Line

Extremely Low Frequency Electromagnetic Fields Alter Expression of C-Myc and circ-CCDC66 in Gastric Cancer Cell Line

Pulsed Magnetic Fields Cause hes1 and hsa-Circ-0068530 Expression Changes in Gastric Cancer Cell Line and Human Normal Fibroblast Cell Line

Design and characterisation of a cell exposure system with high magnetic field homogeneity: RILZ coils

Contact Info

Product

Resources

About