Changes in osteocyte density correspond with changes in osteoblast and osteoclast activity in an osteoporotic sheep model

Zarrinkalam, M. R.; Mulaibrahimovic, Adnan; Atkins, Gerald J.; Moore, Robert J.

doi:10.1007/s00198-011-1672-4

Cited by 50 publications

(33 citation statements)

References 49 publications

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“…Next, the osteocyte lacunar density was assessed, which measures the number of osteocytes per unit of bone area. 33 In this situation, the osteocyte lacunar density indicated both new bone formation and/or repopulation of the DBX particle lacunae. To calculate the osteocyte lacunar density, random high-powered images of H&E-stained slides for each group were used to determine the number of osteocytes per mm 2 of bone area.…”

Section: Isolation and In Vitro Differentiation Of Hpscmentioning

confidence: 97%

Human Perivascular Stem Cells Show Enhanced Osteogenesis and Vasculogenesis with Nel-Like Molecule I Protein

Askarinam¹,

James

Zara

et al. 2013

Tissue Engineering Part A

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An ideal mesenchymal stem cell (MSC) source for bone tissue engineering has yet to be identified. Such an MSC population would be easily harvested in abundance, with minimal morbidity and with high purity. Our laboratories have identified perivascular stem cells (PSCs) as a candidate cell source. PSCs are readily isolatable through fluorescent-activated cell sorting from adipose tissue and have been previously shown to be indistinguishable from MSCs in the phenotype and differentiation potential. PSCs consist of two distinct cell populations: (1) pericytes (CD146 + , CD34 -, and CD45 -), which surround capillaries and microvessels, and (2) adventitial cells (CD146 -, CD34 + , and CD45 -), found within the tunica adventitia of large arteries and veins. We previously demonstrated the osteogenic potential of pericytes by examining pericytes derived from the human fetal pancreas, and illustrated their in vivo trophic and angiogenic effects. In the present study, we used an intramuscular ectopic bone model to develop the translational potential of our original findings using PSCs (as a combination of pericytes and adventitial cells) from human white adipose tissue. We evaluated human PSC (hPSC)-mediated bone formation and vascularization in vivo. We also examined the effects of hPSCs when combined with the novel craniosynostosis-associated protein, Nel-like molecule I (NELL-1). Implants consisting of the demineralized bone matrix putty combined with NELL-1 (3 mg/mL), hPSC (2.5 · 10 5 cells), or hPSC + NELL-1, were inserted in the bicep femoris of SCID mice. Bone growth was evaluated using microcomputed tomography, histology, and immunohistochemistry over 4 weeks. Results demonstrated the osteogenic potential of hPSCs and the additive effect of hPSC + NELL-1 on bone formation and vasculogenesis. Comparable osteogenesis was observed with NELL-1 as compared to the more commonly used bone morphogenetic protein-2. Next, hPSCs induced greater implant vascularization than the unsorted stromal vascular fraction from patientmatched samples. Finally, we observed an additive effect on implant vascularization with hPSC + NELL-1 by histomorphometry and immunohistochemistry, accompanied by in vitro elaboration of vasculogenic growth factors. These findings hold significant implications for the cell/protein combination therapy hPSC + NELL-1 in the development of strategies for vascularized bone regeneration.

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Section: Isolation and In Vitro Differentiation Of Hpscmentioning

confidence: 97%

Human Perivascular Stem Cells Show Enhanced Osteogenesis and Vasculogenesis with Nel-Like Molecule I Protein

Askarinam¹,

James

Zara

et al. 2013

Tissue Engineering Part A

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show abstract

“…Osteocyte lacunar density (N.Lc/BV) depends on the skeletal site, distance to bone surface, tissue bone volume fraction, species, race and health status [26][27][28][29][30][31]. Values in recent human studies range from 20, 000-30, 000/mm 3 [31][32][33].…”

Section: Number Of Osteocytes and Their Lacunaementioning

confidence: 99%

Quantifying the osteocyte network in the human skeleton

Buenzli

Sims

2015

Bone

248

186

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“…[4][5][6] In the present work, the results obtained from a wild subspecies are compared for the first time with those from the corresponding domestic subspecies in order to high- Moreover, the femur of sheep brings on some interest because it represents an excellent experimental model to better understand the development and evolution of some human bone diseases, such as osteoporosis and fracture. [7][8][9][10] Finally, the comparison between mouflon and sheep here proposed may represent an example of recognizing bones from different animals, which may be useful in forensic exam and archaeozoological studies.…”

Section: Introductionmentioning

confidence: 99%

Comparative histology of the femur between mouflon (Ovis aries musimon) and sheep (Ovis aries aries)

et al. 2014

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Mouflon (Ovis aries musimon) and sheep (Ovis aries aries) are considered as the wild and domestic subspecies of the same species. A comparative study on the microstructure of mouflon and sheep femoral bone diaphysis is here reported. Bone microstructure is described for the first time in the mouflon. More than 200 secondary osteons from both subspecies were analyzed and qualitative evaluation was followed by quantitative determination of perimeter, area, minimum and maximum diameters of secondary osteons and Haversian canals. The basic structural patterns observed in both subspecies can be classified as plexiform and irregular Haversian tissue, in accordance with what reported in the literature for most ruminants. The presence of many secondary osteons in the mouflon means that the bone also consists of dense Haversian bone tissue. Statistical analysis demonstrated that mouflon secondary osteons are larger than in the sheep and made of a greater number of lamellae. Since mouflon and sheep are taxonomically closely related and their body size is very similar, the qualitative and quantitative differences here reported could be primarily explained on account of their different lifestyle. Indeed, the habits of wildlife typical of mouflons may lead to the presence of wide areas of dense Haversian tissue in that subspecies, as mechanical stresses are known to be related to number and size of secondary osteons. Finally, this analysis could provide a useful tool to recognize bones from different species, in forensic exam and archaeozoological studies as well.

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Changes in osteocyte density correspond with changes in osteoblast and osteoclast activity in an osteoporotic sheep model

Cited by 50 publications

References 49 publications

Human Perivascular Stem Cells Show Enhanced Osteogenesis and Vasculogenesis with Nel-Like Molecule I Protein

Human Perivascular Stem Cells Show Enhanced Osteogenesis and Vasculogenesis with Nel-Like Molecule I Protein

Quantifying the osteocyte network in the human skeleton

Comparative histology of the femur between mouflon (Ovis aries musimon) and sheep (Ovis aries aries)

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