Changes in oxidative stress levels during two weeks of smoking cessation treatment and their association with nutritional characteristics in Japanese smokers

Abstract

Although several experimental studies have reported that oxidative stress levels decrease during smoking cessation, how they change among general smokers has yet to be completely elucidated. In the present study, a total of 23 smokers who underwent smoking cessation treatment were observed for two-week changes in their levels of 8-OHdG and 8-isoprostane. Physical and nutritional characteristics were measured at the initial patient visit, and casual urine samples were collected at the initial visit and at a follow-up visit two weeks later. Oxidative stress was measured by a high performance liquid chromatography electrochemical detector, and the two-week difference in the levels of oxidative stress was assessed according to demographic and nutrient factors. Neither the urinary level of 8-OHdG nor that of 8-isoprostane decreased, although the cotinine level was decreased at two weeks. A Two-way repeated ANOVA revealed a significant interaction for fat intake by time for the change in the 8-OHdG level (P=0.03) and significant interactions for α-tocopherol intake (P=0.03), iron intake, and carbohydrate intake (P=0.03), all of which were time-dependent for the change in the 8-isoprostane level. The 8-OHdG level decreased among smokers with a high fat intake and was increased with a low fat intake. The 8-isoprostane levels were decreased among smokers with a high carbohydrate intake and increased with a low carbohydrate intake, decreased with a low iron intake and increased with a high iron intake and decreased with a low α-tocopherol intake and increased with a high α-tocopherol intake. Although the present study failed to observe a decrease in oxidative stress levels during the two-week smoking cessation period, we hypothesize that the intake levels of specific nutrients when initiating smoking cessation treatment may predict any subsequent changes in the oxidative stress levels.

Evaluation of Oxidative Status in Patients With Chronic Periodontitis and Additional Tobacco Abuse: A Cross-Sectional Study

“…Damage can be caused by direct effects of cigarette smoke and activation of ROS-producing phagocytic cells. [32][33][34] A number of cross-sectional studies have demostrated that the risk of periodontal disease existance is greater in smokers. [35][36][37][38] It is possible to OS may play an important role in the interaction between periodantitis and smoking.…”

Establishment of epigenetic markers to predict irradiation efficacy against oropharyngeal cancer

“…We collected the following clinicopathological factors for all the patients enrolled in this study: age, gender, tumor site, HPV‐L1 status, clinical stage (T, N, stage based on UICC 8th edition), Brinkman index of 200 as smoking behavior, irradiation dose and combination chemotherapy (Table ). The 40 specimens of the training set were collected during biopsies performed before treatment, immediately frozen in liquid nitrogen and stored at −80°C.…”

Impact of modernization on oxidative stress among indigenous populations in northern Laos

“…We measured urinary 8-OHdG and 8-isoprostane concentrations using liquid chromatography-tandem mass spectrometry (LC-MS/ MS) following solid-phase extraction (Inaba et al, 2011;Oba et al, 2019). Urine was processed with a C18 solid-phase extraction cartridge (YMC Co., Ltd., Kyoto, Japan) for both 8-OHdG and 8-isoprostane.…”