Changes in Permeability of Strial Vessels following Vibration Given to Auditory Ossicle by Drill

Abstract

We produced drill-induced damage of the auditory ossicles of guinea pigs to study changes over time in the permeability of the blood vessels of the stria vascularis to horseradish peroxidase (HRP). In group A, the stimulus was applied for 10 seconds after intravenous injection of HRP. In group B, it was applied for 30 seconds, and in group C, for 60 seconds. The cochlea was fixed with 2% glutaraldehyde perfused through the round window, and the guinea pigs were then decapitated. The stria vascularis of the basal and third turns was examined. The leakage of HRP from the blood vessels of the stria vascularis significantly increased in relation to the duration of the stimulus in both the basal and third turns. The damage to intermediate cells also tended to be in relation to the duration of the stimulus. Extravascular permeation of HRP took place through the tubules in the endothelial cytoplasm. The vibratory stimulation presumably opened channels that are not normally open.

Pattern Of Hearing Loss In A Rat Model Of Cochlear Implantation Trauma

“…One study has reported that the intensity of noise generated by a rotating drill burr was safe for hearing (13); however, several other reports have shown that transient variations in hearing thresholds occur in response to the noise generated during surgical drilling (14,15). According to Kylen and colleagues (14), drillinduced noise levels vary with the type, size, and rotation rate of the drill burr used in otologic surgical procedures.…”

“…According to Kylen and colleagues (14), drillinduced noise levels vary with the type, size, and rotation rate of the drill burr used in otologic surgical procedures. A previous study has also shown that changes in the permeability of blood vessels and in the intermediate cells of the stria vasculariz are related to the noise-induced injury generated during surgical drilling (15). During the preliminary study to develop a cochlear implantation trauma model, we first used a drill to open the bulla and to perform a cochleostomy into the scala tympani.…”

Resident macrophages in the cochlear blood-labyrinth barrier and their renewal via migration of bone-marrow-derived cells

“…2g, j). Extravasation of HRP from capillaries has previously been reported by various laboratories (Seki et al 2001;Watanabe and Tanaka 1997). In this study, HRP leakage from capillaries has been assessed as minimal, since peripheral extracellular fluorescence is much weaker than that in PVMs.…”

Vibration induced hearing loss in guinea pig cochlea: expression of TNF-α and VEGF

“…Excessive vibration of temporal bone occurs for example, in head injury or during forceful drilling of temporal bone. By experimental drilling of the incus body, the permeability of the blood vessels of the stria vascularis was increased (Seki et al, 2001). By using electromagnetic shaker, we recently established an animal model to mimic vibration of the temporal bone caused by the surgical drilling (Zou et al, 2001).…”