Changes in photosynthesis, growth and biomass composition in outdoor Chlorella g120 culture during the metabolic shift from heterotrophic to phototrophic cultivation regime

Masojídek, Jiří; Ranglová, Karolína; Rearte, Tomás Agustín; Plá, Paula S.M. Celis; Torzillo, Giuseppe; Benavides, Ana Margarita Silva; Neori, Amir; Gómez-Serrano, Cintia; Álvarez‐Gómez, Félix; Lukeš, Martin; Caporgno, Martín P.; Abdala, Roberto; Miazek, Krystian; Massocato, Thaís Fávero; Silva, Jaqueline Carmo da; Atzmüller, Richard; Mahrouqui, Hafidh Al; Suárez‐Estrella, F.; Figueroa, Félix L.

doi:10.1016/j.algal.2021.102303

Cited by 18 publications

(13 citation statements)

References 47 publications

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“…In the present study, we did not detect significant differences in the values of F v /F m determined at different times of the day for the two algae, suggesting the absence of photoinhibition. These results differ from other studies carried out under similar conditions which observed a decrease in F v /F m at noon (Cruces et al, 2019;Figueroa et al, 2020;Masojıdek et al, 2021), and can be explained by special adaptations of Ulva spp. to light fluctuations.…”

Section: Photosynthetic Performancecontrasting

confidence: 99%

Growth, biofiltration and photosynthetic performance of Ulva spp. cultivated in fishpond effluents: An outdoor study

et al. 2022

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Anthropogenic impacts on water resources, especially by nutrient discharge, is a worldwide problem in marine coastal areas. In this context, seaweed cultivation in aquaculture wastewater can be considered as an alternative for effluent mitigation, where the biomass becomes a source of valuable compounds. The current study examined the potential use of the seaweeds Ulva pseudorotundata and Ulva rigida to remove nutrients to treat effluents from the culture of Chelon labrosus. Two experiments were conducted under pilot-scale conditions to evaluate the nutrient uptake, photosynthetic activity, and biomass production of the seaweed species cultivated under 50 and 100% effluent concentrations. Photosynthetic parameters were determined by in vivo chlorophyll a fluorescence associated to photosystem II 3 times a day to estimate photosynthetic performance and seaweed physiology throughout the experiment: optimal quantum yield (Fv/Fm), in situ and ex situ electron transport rate (ETR), photosynthetic efficiency (αETR), saturation irradiance (Ek), and the maximum non-photochemical quenching (NPQmax). To evaluate seaweed metabolism and biomass compounds, elemental and biochemical composition were analyzed in the beginning and end of each experiment. Results regarding the nutrient source showed that both species removed more than 65% of ammonium after 3 hours of experimentation. At the end of the experiments, up to 94.8% of the initial ammonium was sequestered from the effluent. Additionally, after 5 days of cultivation under 50% fish effluent both Ulva species were able to remove more than 85% of the nitrate. Although a decrease in uptake efficiency was observed in cultures with 100% fish effluent, at the end of the experiment more than 440 µmol L-1 of nitrate was removed, considering all treatment conditions. The biomass values showed that growth rates of seaweed cultivated in 100% effluent were higher than those obtained in 50% effluent. Moreover, when cultivated in the 100% effluent concentration, a significant increment in protein content was detected in both Ulva species. Our results contribute to the understanding of biofiltration and photosynthetic performance of two different Ulva species in order to improve growth optimization, enhancement of biofiltration capacity and also to boost management practices of seaweed cultivation in aquaculture effluent treatment systems.

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Section: Photosynthetic Performancecontrasting

confidence: 99%

Growth, biofiltration and photosynthetic performance of Ulva spp. cultivated in fishpond effluents: An outdoor study

et al. 2022

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“…However, as the culture become denser, and thus the available light is reduced, the productivity of the reduced antenna size strain is overhauled by WTs [62]. Similar results were also obtained outdoor in thin-layer cascades comparing two Chlorella strains with different antenna sizes [63]. Thus, a strain with the reduced antenna size is not sufficient to guarantee higher biomass productivity in mass outdoor culture.…”

Section: Changing Antenna Sizementioning

confidence: 58%

“…This was suggested by Benemann [56][57][58][59], showing that reduced pigment content resulted in dense laboratory cultures with higher photosynthetic activity. Cells with a reduced pigment content, mutants with a smaller antenna size or transgenic microalgae with reduced antenna confirmed that this concept works at the laboratory scale size [60][61][62][63][64][65][66]. However, results in outdoor cultivation units are still ambiguous.…”

Section: Changing Antenna Sizementioning

confidence: 83%

“…The culture heated in the early morning showed a significant increase in productivity of about 60% compared to the non-heated culture. The synergism of sub-optimal temperature and/or high DO concentrations (200-400% saturation) with excess irradiance can cause a decrease in photosynthetic activity and growth [63,97,[105][106][107]. It happens when the rise in light intensity is much faster than the rise in temperature, with a delay of hours, or the build-up of DO concentration due to high photosynthetic activity is enormous in a culture unit.…”

Section: Dissolved Oxygenmentioning

confidence: 99%

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Variables Governing Photosynthesis and Growth in Microalgae Mass Cultures

et al. 2021

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Since the 1950s, microalgae have been grown commercially in man-made cultivation units and used for biomass production as a source of food and feed supplements, pharmaceuticals, cosmetics and lately biofuels, as well as a means for wastewater treatment and mitigation of atmospheric CO2 build-up. In this work, photosynthesis and growth affecting variables—light intensity, pH, CO2/O2 exchange, nutrient supply, culture turbulence, light/dark cell cycling, biomass density and culture depth (light path)—are reviewed as concerns in microalgae mass cultures. Various photosynthesis monitoring techniques were employed to study photosynthetic performance to optimize the growth of microalgae strains in outdoor cultivation units. The most operative and reliable techniques appeared to be fast-response ones based on chlorophyll fluorescence and oxygen production monitoring, which provide analogous results.

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“…For ex-situ measurements of Chl fluorescence and oxygen production, microalgae samples were taken from outdoor cultures at specified time intervals (9:00, 13:00, and 17:00 h) as described previously [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors

Masojídek

Gómez-Serrano

Ranglová

et al. 2022

Biology

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Microalgae cultures were used for a WW treatment to remediate nutrients while producing biomass and recycling water. In these trials, raceway ponds (RWPs; 1 and 0.5 ha) were located next to a municipal (WW) treatment plant in Mérida, Spain. The ponds were used for continuous, all-year-round microalgae production using WW as a source of nutrients. Neither CO2 nor air was supplied to cultures. The objective was to validate photosynthesis monitoring techniques in large-scale bioreactors. Various in-situ/ex-situ methods based on chlorophyll fluorescence and oxygen evolution measurements were used to follow culture performance. Photosynthesis variables gathered with these techniques were compared to the physiological behavior and growth of cultures. Good photosynthetic activity was indicated by the build-up of dissolved oxygen concentration up to 380% saturation, high photochemical yield (Fv/Fm = 0.62–0.71), and relative electron transport rate rETR between 200 and 450 μmol e− m−2 s−1 at midday, which resulted in biomass productivity of about 15–25 g DW m−2 day−1. The variables represent reliable markers reflecting the physiological status of microalgae cultures. Using waste nutrients, the biomass production cost can be significantly decreased for abundant biomass production in large-scale bioreactors, which can be exploited for agricultural purposes.

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Changes in photosynthesis, growth and biomass composition in outdoor Chlorella g120 culture during the metabolic shift from heterotrophic to phototrophic cultivation regime

Cited by 18 publications

References 47 publications

Growth, biofiltration and photosynthetic performance of Ulva spp. cultivated in fishpond effluents: An outdoor study

Growth, biofiltration and photosynthetic performance of Ulva spp. cultivated in fishpond effluents: An outdoor study

Variables Governing Photosynthesis and Growth in Microalgae Mass Cultures

Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors

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