Changes in plasma glucose, amino acid nitrogen and growth hormone during smoltification and seawater adaptation in coho salmon, Oncorhynchus kisutch

Sweeting, R. M.; Wagner, Graham F.; McKeown, B.A.

doi:10.1016/0044-8486(85)90269-8

Cited by 82 publications

(35 citation statements)

References 30 publications

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“…The circulating GH increases during the transfer of salmon from fresh water to seawater, whether in seawater challenge experiments or during natural migration to seawater (Sweeting et al 1985, Young et al 1989. GH can also reduce the plasma sodium concentration (Bolton et al 1987, Collie et al 1989, and increase gill Na + /K + -ATPase activity in salmonids (Bjornsson et al 1987, Richman & Zaugg 1987.…”

Section: Discussionmentioning

confidence: 99%

Quantification of common carp (Cyprinus carpio) IGF-I and IGF-II mRNA by real-time PCR: differential regulation of expression by GH

Vong

Chan²,

Chen

2003

Journal of Endocrinology

140

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IGFs are potent mitogens for many different cell types and play important roles in growth and development. A multitude of regulatory factors modulate the expression of IGFs. In some teleosts, liver IGF-I expression has been demonstrated to depend on the presence of GH. However, the GH dependence of IGF-II expression in teleosts is controversial. Moreover, most IGF expression studies in bony fish have been focused on the liver, and information on extrahepatic tissues are conflicting and inconsistent. This is partly due to the fact that the traditional methods of mRNA measurement such as Northern blot and RT-PCR are not sensitive enough to detect changes in IGF levels in extrahepatic tissues because of the low levels of IGFs in these tissues. In addition, there have been few studies on the IGF system of non-salmonid teleosts. Our laboratory has thus begun such studies on a local tropical fast-growing fish, the common carp (Cyprinus carpio). In this study, real-time quantitative PCR assays were developed for the accurate measurement of IGF-I and IGF-II mRNA levels in common carp tissues. This quantitative method was based on the measurement of a fluorescent labeled probe, which was cleaved by Taq polymerase during PCR by the 5 <3 nuclease activity. The signal generated was directly proportional to the starting copy number of the target molecules in the sample. Hence, it was possible to detect and quantify the mRNA levels of both IGF-I and IGF-II reliably in very small amounts of tissues obtained from juvenile common carp. Using these assays, the expression pattern of IGF-I and IGF-II in various common carp tissues was studied, and their differential response to GH stimulation was also investigated.

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Section: Discussionmentioning

confidence: 99%

Quantification of common carp (Cyprinus carpio) IGF-I and IGF-II mRNA by real-time PCR: differential regulation of expression by GH

Vong

Chan²,

Chen

2003

Journal of Endocrinology

140

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“…Furthermore, no specific assay is available to determine IGF-I protein levels in fishes since IGF-I protein has not been obtained from teleosts; only the cDNA of coho salmon IGF-I is known (21). Therefore, we examined the effect of GH on IGF-I mRNA levels in the liver, gill, and body kidney and their changes during seawater adaptation of rainbow trout, when GH levels are known to increase (14)(15)(16)(17)(18)(19).…”

mentioning

confidence: 99%

“…Bolton et al (12) and Collie et al (13) have demonstrated that the effect of GH on seawater adaptation of rainbow trout (Oncorhynchus mykiss) is independent of the growth-promoting actions of GH. Plasma levels and turnover rates of GH increase after transfer of several salmonids to seawater (14)(15)(16)(17)(18)(19). Recently, we have shown that bovine IGF-I also enhances the hypoosmoregulatory ability of rainbow trout by inhibiting the increase in plasma osmolarity and sodium levels after transfer to seawater, independently of its growth-promoting effect (20).…”

mentioning

confidence: 99%

Expression of insulin-like growth factor I gene in osmoregulatory organs during seawater adaptation of the salmonid fish: possible mode of osmoregulatory action of growth hormone.

Sakamoto

Hirano

1993

Proc. Natl. Acad. Sci. U.S.A.

160

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“…The growth hormone form parameters allowing smolts to survive in the seawater [3][4][5][6]9,10]. It is shown [11] that the hypophysectomized young coho salmon and young chinook survive in freshwater but die in seawater.…”

Section: The Processes Connected With Preparation Of Young Salmonid Fmentioning

confidence: 99%

“…During smoltification hypertrophy and hyperplasia of hypophysis somatotropic cells occurs resulted in increased excretion of growth hormone [1][2][3][4] leading to rising of its concentration in blood [1,[5][6][7][8]. The growth hormone form parameters allowing smolts to survive in the seawater [3][4][5][6]9,10].…”

Section: The Processes Connected With Preparation Of Young Salmonid Fmentioning

confidence: 99%

Physiological and biochemical basis of salmon young fishes migratory behavior

Martemyanov¹,

Oblast²

2016

J Coast Life Med

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Changes in plasma glucose, amino acid nitrogen and growth hormone during smoltification and seawater adaptation in coho salmon, Oncorhynchus kisutch

Cited by 82 publications

References 30 publications

Quantification of common carp (Cyprinus carpio) IGF-I and IGF-II mRNA by real-time PCR: differential regulation of expression by GH

Quantification of common carp (Cyprinus carpio) IGF-I and IGF-II mRNA by real-time PCR: differential regulation of expression by GH

Expression of insulin-like growth factor I gene in osmoregulatory organs during seawater adaptation of the salmonid fish: possible mode of osmoregulatory action of growth hormone.

Physiological and biochemical basis of salmon young fishes migratory behavior

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