Changes in plasma oxidative state with age and their influence on contractions elicited by noradrenaline in the rat tail artery

Rodríguez-Martínez, María; García-Cohen, Edith C.; Briones, Ana M.; Baena, Ana B.; Marı́n, Esperanza; Salaíces, Mercedes; Marı́n, Jesús

doi:10.1016/s0024-3205(99)00321-5

Cited by 14 publications

(7 citation statements)

References 33 publications

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“…This is accompanied with marked elevation of GSH in testes, Zn in serum and brain as well as Cu in serum, liver, brain and testes in antioxidant-supplemented rats. The present results confirm those of Rodriguez-Martinez et al [13] who demonstrated enhancement of plasma oxidative state accompanied with decreased glutathione peroxidase activity in 24-month-old rats and suggested an important role in the increase of vascular resistance with age.…”

Section: Discussionsupporting

confidence: 93%

Modulation of Age-Related Biochemical Changes and Oxidative Stress by Vitamin C and Glutathione Supplementation in Old Rats

Amer

2002

Ann Nutr Metab

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The present study sought to determine whether supplementation of dietary antioxidant ascorbic acid with glutathione (GSH) could ameliorate the age-related increased oxidative stress and changes in hormonal, lipid and copper (Cu) as well as zinc (Zn) levels in 18-month-old rats. The present study demonstrated that supplementation of vitamin C (30 mg) + GSH 100 mg/kg b.w. significantly reduced the concentration of thiobarbituric acid-reactive substances in liver and testes in old male rats as compared with nonsupplemented ones, indicating lower oxidative stress. In addition, testicular GSH was increased but not hepatic GSH. Also, cholesterol and triglycerides were decreased in the serum of supplemented rats. Furthermore, she serum testosterone level was increased in the same supplemented rats. However, the present results show that the thyroid hormones, T₃ and T₄, were not influenced. Lastly, the concentration of Cu in serum, liver, brain and testes was increased in supplemented old rats. Zn concentration was also increased in the same organs but not in the liver. According to the present study, the supplementation of antioxidants could play an important role in the modulation of the oxidative damage and changes associated with age.

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Section: Discussionsupporting

confidence: 93%

Modulation of Age-Related Biochemical Changes and Oxidative Stress by Vitamin C and Glutathione Supplementation in Old Rats

Amer

2002

Ann Nutr Metab

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“…It is well known that lipid peroxidation increase with ageing. Rodriguez‐Martinez et al found a progressive increase in plasma MDA levels and a decrease in plasma anti‐oxidase activity 39 in aged rats. In the present study, we have also found that the serum MDA content is increased in aged rats, which parallels the increase in serum ADMA levels.…”

Section: Discussionmentioning

confidence: 96%

Elevated Serum Endogenous Inhibitor Of Nitric Oxide Synthase And Endothelial Dysfunction In Aged Rats

Xiong

Yuan

Deng

et al. 2001

Clin Exp Pharma Physio

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1. The purpose of the present study was to determine the relationship between endothelial dysfunction and the endogenous inhibitor of nitric oxide synthase NG,NG'-asymmetric dimethylarginine (ADMA) in aged rats. 2. Studies were performed in male adult Sprague-Dawley rats (6 months old; n = 8) and in aged rats (20 months old; n = 8). Serum levels of ADMA and L-arginine were measured by high-performance liquid chromatography and responses of endothelium-intact aortic rings to acetylcholine (ACh) were tested. Nitric oxide synthase activity in kidney tissue and serum concentrations of nitrite, a stable end-product of nitric oxide, were assayed and serum contents of malondialdehyde, derived from lipid peroxidation and serum lipid and creatinine level were determined. 3. Serum levels of ADMA increased significantly in aged rats compared with adult rats (P < 0.01), whereas serum levels of L-arginine were similar in both groups (P = NS). Accordingly, the ratio of L-arginine/ADMA in old rats was lower than that in young rats (P < 0.01). Endothelium-dependent relaxation responses to ACh in aortic rings from aged rats were impaired and these impaired responses were improved by pre-incubation of aortic rings with L-arginine. 4. Nitric oxide synthase activity in the kidney, together with serum concentration of nitrite, was significantly decreased and serum contents of malondialdehyde, cholesterol and triglycerides were increased in old compared with young rats. However, the serum creatinine level was not significantly different between adult and aged rats. 5. Endogenous ADMA may be a contributor to age-related endothelial dysfunction and increases in endogenous ADMA may be linked to lipid peroxidation in aged rats.

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“…13 In our study no significant correlation between the stress hormone and the lipid oxidation products conjugated dienes and MDA could be observed after exercise, although it has been suggested that auto-oxidation of catecholamines, which generates a superoxide anion radical, could result in lipid oxidation. 15 During short term resistance exercise, noradrenaline concentrations increase only moderately. We cannot rule out the possibility that during intensive endurance exercise, high noradrenaline concentration may contribute to oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Correlations between plasma noradrenaline concentrations, antioxidants, and neutrophil counts after submaximal resistance exercise in men

Ramel

Wagner²,

Elmadfa³

2004

Br J Sports Med

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Background: Generation of reactive oxygen species (ROS) during exercise has been linked to increased oxygen consumption. ROS could also be produced by other mechanisms-for example, a respiratory burst of neutrophils or catecholamine auto-oxidation-when oxygen consumption is only moderately increased. Objectives: To investigate noradrenaline concentrations, neutrophil counts, plasma antioxidants, and lipid oxidation products before and after acute resistance exercise. Methods: 17 male participants undertook a submaximal resistance exercise circuit (10 exercises; 75% of the one repetition maximum; mean (SD) exercise time, 18.6 (1.1) minutes). Blood samples were taken before and immediately after exercise and analysed for plasma antioxidants, noradrenaline, neutrophils, and lipid oxidation products. Wilcoxon's signed-rank test and Pearson's correlation coefficient were used for calculations. Results: Neutrophils, noradrenaline, fat soluble antioxidants, and lipid oxidation products increased after exercise. Noradrenaline concentrations were associated with higher antioxidant concentrations. Neutrophils were related to higher concentrations of conjugated dienes. Conclusions: Submaximal resistance exercise increases plasma antioxidants. This might reflect enhanced antioxidant defence in response to the oxidative stress of exercise, though this is not efficient for inhibiting lipid oxidation. The correlation between noradrenaline concentrations and plasma antioxidants suggests a modulating role of the stress hormone. Neutrophils are a possible source of oxidative stress after resistance exercise.

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Changes in plasma oxidative state with age and their influence on contractions elicited by noradrenaline in the rat tail artery

Cited by 14 publications

References 33 publications

Modulation of Age-Related Biochemical Changes and Oxidative Stress by Vitamin C and Glutathione Supplementation in Old Rats

Modulation of Age-Related Biochemical Changes and Oxidative Stress by Vitamin C and Glutathione Supplementation in Old Rats

Elevated Serum Endogenous Inhibitor Of Nitric Oxide Synthase And Endothelial Dysfunction In Aged Rats

Correlations between plasma noradrenaline concentrations, antioxidants, and neutrophil counts after submaximal resistance exercise in men

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