Changes in polysome content during development after diapause of <i>Bombyx mori</i> embryos

Saito, Akira; Koga, Katsumi; Sakaguchi, Bungo

doi:10.1016/0014-5793(82)80787-4

Cited by 3 publications

(1 citation statement)

References 16 publications

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“…Lower levels of ribosomal proteins and elongation factors during diapause agree well with previous reports on diapause protein production capacity and cell growth [27] . Similarly, there is an established link between histone expression and replication/translation [28] .…”

Section: Discussionsupporting

confidence: 92%

Deciphering Proteomic Signatures of Early Diapause in Nasonia

Wolschin

Gadau

2009

PLoS ONE

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Insect diapause is an alternative life-history strategy used to increase longevity and survival in harsh environmental conditions. Even though some aspects of diapause are well investigated, broader scale studies that elucidate the global metabolic adjustments required for this remarkable trait, are rare. In order to better understand the metabolic changes during early insect diapause, we used a shotgun proteomics approach on early diapausing and non-diapausing larvae of the recently sequenced hymenopteran model organism Nasonia vitripennis. Our results deliver insights into the molecular underpinnings of diapause in Nasonia and corroborate previously reported diapause-associated features for invertebrates, such as a diapause-dependent abundance change for heat shock and storage proteins. Furthermore, we observed a diapause-dependent switch in enzymes involved in glycerol synthesis and a vastly changed capacity for protein synthesis and degradation. The abundance of structural proteins and proteins involved in protein synthesis decreased with increasing diapause duration, while the abundance of proteins likely involved in diapause maintenance (e.g. ferritins) increased. Only few potentially diapause-specific proteins were identified suggesting that diapause in Nasonia relies to a large extent on a modulation of pre-existing pathways. Studying a diapause syndrome on a proteomic level rather than isolated pathways or physiological networks, has proven to be an efficient and successful avenue to understand molecular mechanisms involved in diapause.

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Section: Discussionsupporting

confidence: 92%

Deciphering Proteomic Signatures of Early Diapause in Nasonia

Wolschin

Gadau

2009

PLoS ONE

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Presence of translatable mRNA in diapause eggs of Bombyx mori

Saito

Kiyohara

Sugimoto³

et al. 1984

Insect Biochemistry

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Translation of RNA from Eggs During Post‐Diapause Development of Bombyx mori

Saito¹,

Sugimoto²,

Koga³

et al. 1985

Dev Growth Differ

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Eggs of Bombyx mori are aroused from diapause by long-term chilling and develop when transferred to 25°C. During the first 20 hr of post-diapause development, the polysome content and the presumed rate of protein synthesis increase about 3-fold, while the ribosome content and the total RNA content increase only 1.1-fold. In this study, total RNAs were extracted from chilled eggs (termed 0 hr of development), and post-diapause eggs at 10 and 20 hr of development. The RNAs were purified further by high pressure liquid chromatography to remove RNA-like oligonucleotides. On translation in a protein-synthesizing system derived from wheat germ with a subsaturating amount of RNA, no difference was found in the relative amounts of translatable mRNA activity at 10 and 20 hr of development from that at 0 hr. Moreover, the translation products of the different RNA preparations in a rabbit reticulocyte lysate system appeared very similar when separated by gel electrophoresis and located by fluorography. These facts suggest that protein synthesis in early post-diapause development is controlled at a translational level.In the silkworm, Bombyx mori, embryogenesis is suspended at an early stage before the onset of neural groove invagination, if the embryo is fated to enter diapause. Diapause terminates after chilling the eggs at 5°C for 3 to 4 months, and the eggs then undergo post-diapause development when kept at 25"C, hatching after 10 days. The rate of protein synthesis increases about 3-fold during the first 20 hr of post-diapause development, as judged by increase in the content of polysomes and in the rate of incorporation of radioactive amino acid into total proteins by fragmented embryos cultured in vitro (1). The bulk RNA and ribosome contents gradually increase to 3-fold the initial levels by the end of embryogenesis. During post-diapause development, the neural groove becomes observable at 24 hr and protein synthesis is likely to be activated before the onset of visual morphogenetic progresses (1).GRZELAK et ul. (2) reported that poly(A)+RNA extracted from dormant (chilled) Bombyx eggs via polysome-like aggregates was completely inactive in a wheat germ cell-free proteinsynthesizing system in vitro. They thought that mRNAs were defective in translatability in these eggs and that some modification of the inactive mRNAs was necessary for their translation during subsequent development. On the other hand, we recently found that total RNAs extracted from eggs both during chilling and during diapause could be translated in wheat germ and rabbit reticulocyte systems (3). These findings, which imply that wintering eggs contain a store of messages that are active in vitro, prompted us to compare the translatabilities of RNAs from chilled and developing eggs.In the present study, total RNAs extracted from eggs before the beginning of post-diapause development, and 10 and 20 hr of development, were assayed in protein-synthesizing systems 13

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Changes in polysome content during development after diapause of Bombyx mori embryos

Cited by 3 publications

References 16 publications

Deciphering Proteomic Signatures of Early Diapause in Nasonia

Deciphering Proteomic Signatures of Early Diapause in Nasonia

Presence of translatable mRNA in diapause eggs of Bombyx mori

Translation of RNA from Eggs During Post‐Diapause Development of Bombyx mori

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