Changes in proteomic cargo of circulating extracellular vesicles in response to lifestyle intervention in adolescents with hepatic steatosis

DiStefano, Johanna K.; Piras, Ignazio S.; Wu, Xiumei; Sharma, Ritin; Garcia-Mansfield, Krystine; Willey, Maya; Lovell, Brooke; Pirrotte, Patrick; Olson, Micah L.; Shaibi, Gabriel Q.

doi:10.1016/j.clnesp.2024.02.024

Cited by 1 publication

(2 citation statements)

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“…Exclusion Criteria. (1) Patients currently taking NSAIDs more than 3/week on a prescription basis or taking a daily dose of NSAID; (2) history of diabetes and patients taking diabetes medications as these drugs may alter adipose tissue metabolic functions; (3) history of uncontrolled hypertension defined as >160 systolic and 95 diastolic on medication; (4) history of renal disease with GFR < 60; (5) history of hepatic failure or AST/ALT > three times the normal range; (6) patients with active cancer within the last 2 years except for skin cancers; (7) patients with acute illness needing hospitalization within the last 2 months; (8) patients with acute inflammation; (9) patients with cardiovascular events such as myocardial infarction, stroke, amputation, unstable angina within the last six months; (10) pregnancy; and (11) presence of psychosis, suicidal ideations, untreated major depression, dementia and history of stimulant dependence/substance abuse. The study was approved by the Institutional Review Board at the VA Nebraska-Western Iowa Health Care System (NWIHCS).…”

Section: Study Design and Subjectsmentioning

confidence: 99%

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A Pilot Study on the Proteomics Profile of Serum Exosome-Enriched Extracellular Vesicles from Normal versus Individuals with Obesity-Related Insulin Resistance

Saraswathi,

Ai,

Kumar

et al. 2024

Biomedicines

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Objective: Circulating exosome-enriched extracellular vesicles (EVs) have drawn considerable importance in obesity-related insulin-resistance (IR). We sought to compare the proteomics profile of serum exosomes from normal individuals and those with obesity and IR. Methods: We isolated serum exosomes from male subjects with obesity and insulin resistance (Ob-IR, HOMA-IR > 2.0) and lean/overweight insulin-sensitive (Normal (N), HOMA-IR < 2.0) individuals. The differential protein expression between the two groups was detected by a label-free quantitative mass spectrometry analysis followed by GO annotation and ingenuity pathway analysis (IPA). Results: We identified 23 upregulated and 46 downregulated proteins between Ob-IR and N groups. Some of these proteins are involved in altering insulin signaling (VPS13C, TBC1D32, TTR, and ADIPOQ), inflammation (NFκB and CRP), and B-cell proliferation/activation (IGLV4-69, IGKV1D-13, and IGHV4-28). GO analysis revealed that the differentially expressed proteins (DEPs) are mainly involved in regulating immune cell activation and are located in extracellular space. IPA analysis showed that top molecules mediating IR, inflammation and B-cell activation were upregulated in Ob-IR subjects compared to N subjects. Conclusions: Serum exosomal proteins can be used as biomarkers to identify the future risk of diabetes and a therapeutic target to prevent or slow down the progression of diabetes in high-risk individuals.

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Section: Study Design and Subjectsmentioning

confidence: 99%

“…In addition to serving as a biomarker, the exosomal proteomics profile may also reveal the therapy response. For example, in a recent study, plasma exosomal proteomics cargo has been shown to alter in response to lifestyle interventions in adolescents with hepatic steatosis [10].…”

Section: Introductionmentioning

confidence: 99%