Changes in Rat Uterine Estrogen Receptor Messenger Ribonucleic Acid Levels during Estrogen- and Progesterone-Induced Estrogen Receptor Depletion and Subsequent Replenishment1

Rosser, Mary; Chorich, Lynn P.; Howard, Eugene F.; Zamorano, Pedro; Mahesh, Virendra B.

doi:10.1095/biolreprod48.1.89

Cited by 47 publications

(38 citation statements)

References 54 publications

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“…4 C and D). The latter finding is consistent with previous reports (25,37). Uterine ER␣ levels were remarkably low in ovariectomized females on the Purina 5001 diet (Fig.…”

Section: Commercial Diets Alter Estrogen-responsive Gene Expression Isupporting

confidence: 93%

“…These changes are similar in nature to those previously observed for native estrogen (7,(25)(26)(27)(28)(29)(30).…”

Section: Commercial Diets Alter Estrogen-responsive Gene Expression Isupporting

confidence: 87%

“…Estrogen normally down-regulates ER␣ because of enhanced receptor processing and degradation (25). Using immunohistochemistry, we indeed observed that dietary phytoestrogens have pronounced inhibitory effects on ER␣ expression (Fig.…”

Section: Commercial Diets Alter Estrogen-responsive Gene Expression Imentioning

confidence: 55%

“…Thus, it is conceivable that chronic exposure to dietary phytoestrogens would also influence uterine expression of estrogen-responsive genes. We used comparative RT-PCR to analyze uterine mRNA levels of LF, PR, LIF, ER␣, and sFRP2, all well known estrogen-target genes (7,(25)(26)(27)(28)(29)(30), in adult ovariectomized mice after they were fed diets containing low (5V00 diet) or high (5001 diet) phytoestrogens. There was increased uterine accumulation of LF, PR, and LIF mRNAs in mice consuming the Purina 5001 diet with concurrent decreased levels of ER␣ and sFRP2 (Fig.…”

Section: Commercial Diets Alter Estrogen-responsive Gene Expression Imentioning

confidence: 99%

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Variation in commercial rodent diets induces disparate molecular and physiological changes in the mouse uterus

Wang

Tranguch²,

Xie³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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Although ovarian estrogen, estradiol-17␤, is a key modulator of normal reproductive functions, natural and synthetic compounds with estrogen-like activities can further influence reproductive functions. Plant-derived phytoestrogens specifically have received much attention because of associated health benefits. However, a comprehensive understanding of the beneficial and͞or detrimental impacts of phytoestrogen consumption through commercial rodent diets on uterine biology and early pregnancy at the molecular level remains largely unexplored. Using multiple approaches, we demonstrate here that exposure of adult female mice to a commercial rodent diet with higher phytoestrogen levels facilitates uterine growth in the presence or absence of ovarian estrogen, alters uterine expression of estrogen-responsive genes, and advances the timing of implantation compared with a diet with lower phytoestrogen levels. The finding that variability in phytoestrogen content in commercial rodent diets, both within and between brands, influences experimental results stresses the importance of this investigation and raises caution for investigators using rodents as animal models.dietary phytoestrogen ͉ embryo implantation ͉ uterine gene expression

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“…4 C and D). The latter finding is consistent with previous reports (25,37). Uterine ER␣ levels were remarkably low in ovariectomized females on the Purina 5001 diet (Fig.…”

Section: Commercial Diets Alter Estrogen-responsive Gene Expression Isupporting

confidence: 93%

“…These changes are similar in nature to those previously observed for native estrogen (7,(25)(26)(27)(28)(29)(30).…”

Section: Commercial Diets Alter Estrogen-responsive Gene Expression Isupporting

confidence: 87%

Section: Commercial Diets Alter Estrogen-responsive Gene Expression Imentioning

confidence: 55%

Section: Commercial Diets Alter Estrogen-responsive Gene Expression Imentioning

confidence: 99%

See 2 more Smart Citations

Variation in commercial rodent diets induces disparate molecular and physiological changes in the mouse uterus

Wang

Tranguch²,

Xie³

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

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“…Furthermore, the apparent decrease in the total number of measurable binding sites after a hormone challenge in rat uteri is due to degradation of the protein [20,39]. This "processing" found in the regulatory action of E2 on its receptor was also reported in the same specie by other authors [3,13,33], but no data as such has been reported for sheep. The heterologous down-regulation observed also in PR uterine expression after E2 treatment in lambs [22] was unforeseen, and no mechanism of action for this has yet been proposed.…”

Section: Introductionmentioning

confidence: 70%

A biphasic action of estradiol on estrogen and progesterone receptor expression in the lamb uterus

et al. 2000

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-Regulation of the uterine expression of estrogen and progesterone receptors was studied in 20 three-month-old lambs that were not treated or treated with estradiol-17β. Determinations of receptors were performed by binding assays in the nuclear and cytosolic fractions, receptor mRNAs by solution hybridization, and estrogen receptor protein by an enzyme-immunoassay. Estradiol treatment decreased the receptor binding capacity of both receptors and the levels of immunoreactive estrogen receptor 12 h after injection in the absence of decreased receptor mRNAs, suggesting that the initial decrease is due to degradation of the proteins or that mRNAs are translated into new receptor proteins at a reduced rate. The mRNA levels increased after estradiol treatment suggesting that the replenishment phase consists of synthesis of new receptors rather than recycling of inactivated receptors.estrogen / progesterone / receptor / uterus / lamb Résumé -Action biphasique de l'oestradiol sur l'expression des récepteurs aux oestrogènes et à la progestérone dans l'utérus de l'agnelle. La régulation de l'expression des récepteurs aux oestrogènes et à la progestérone au niveau de l'utérus a été étudiée chez des agnelles immatures âgées de 3 mois (n = 20), traitées et non traitées avec oestradiol-17β. Les déterminations des récepteurs ont été faites par des essais de liaison dans les fractions nucléaires et cytosoliques, les ARNm des récepteurs par la méthode d'hybridation en solution et la protéine du récepteur aux oestrogènes par un essai immuno-enzymatique. Le traitement à l'oestradiol réduit la capacité de liaison des deux récepteurs et les niveaux des récepteurs aux oestrogènes immunoréactifs 12 h après l'injection en absence d'une diminution des ARNm des récepteurs, suggèrent que la réduction initiale est due à la dégradation des Reprod. Nutr. Dev. 40 (2000) 283-293 283

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The Effect of Maternal Malnutrition During Lactation on the Endometrial ERα Expression, Collagen Type, and Blood Vessels in the Rats Offspring at Puberty

Brasil

Faria

Sampaio

et al. 2009

The Anatomical Record

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The aim of this manuscript was to evaluate the effects of maternal protein-energy-restriction and energy restriction during lactation on endometrial collagen and blood vessels, uterus Era expression, and estradiol serum levels in the rats offspring at puberty. At parturition, dams were grouped as: control group (C), with free access to standard rat chow containing 23% protein and 17,038.7 KJ/Kg; protein-energy restricted group (PER), with free access to formulated chow containing 8% protein but made isoenergetic to the C diet (17,038.7 KJ/Kg); and energy-restricted group (ER), which received standard rat chow containing 23% protein based on the mean ingestion of the PER group corresponding to 60% of that consumed by the control group. After weaning, all female pups had free access to standard laboratory chow until puberty, when they were killed at the diestrum stage. The uterine ERa expression was determined by Western-Blot and estradiol serum levels by radioimmunoassay. Endometrial collagen and blood vessels were quantified by stereology. The volumetric density of blood vessels (C ¼ 70.7 AE 2.2; PER ¼ 29.2 AE 2.4; ER ¼ 32.3 AE 3.6; P < 0.001) and endometrial collagen (C ¼ 31.1 AE 1; PER ¼ 26.9 AE 1.0; ER ¼ 26.5 AE 0.7; P < 0.05) were significantly reduced in both malnourished groups. The ER group presented higher estradiol serum levels (C ¼ 69.2 AE 6.4; PER ¼ 73.4 AE 5.5; ER ¼ 101.0 AE 5.4; P < 0.01) in relation to C and PER groups. ERa expression was greater in both malnourished groups (C ¼ 0.11 AE 0.02; PER ¼ 0.41 AE 0.12; ER ¼ 0.35 AE 0.03; P < 0.05). In conclusion, maternal malnutrition during lactation caused changes in endometrial angiogenesis, collagen deposition, and Era expression in female offspring that will appear in puberty and could affect the reproductive biology of the female offspring. Anat Rec,

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Changes in Rat Uterine Estrogen Receptor Messenger Ribonucleic Acid Levels during Estrogen- and Progesterone-Induced Estrogen Receptor Depletion and Subsequent Replenishment1

Cited by 47 publications

References 54 publications

Variation in commercial rodent diets induces disparate molecular and physiological changes in the mouse uterus

Variation in commercial rodent diets induces disparate molecular and physiological changes in the mouse uterus

A biphasic action of estradiol on estrogen and progesterone receptor expression in the lamb uterus

The Effect of Maternal Malnutrition During Lactation on the Endometrial ERα Expression, Collagen Type, and Blood Vessels in the Rats Offspring at Puberty

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