Changes in respiratory activities during the cell-cycle of the fission yeast Schizosaccharomyces pombe 972h- growing in the presence of glycerol

Abstract

1. The specific activities of cytochrome c oxidase, catalase, succinate dehydrogenase, succinate-cytochrome c oxidoreductase, NADH-cytochrome c oxidoreductase, and NADPH-cytochrome c oxidoreductase in mid-exponential-phase batch cultures of glycerol-grown Schizosaccharomyces pombe indicated that the organisms were catabolite-de-repressed. 2. In cultures growing synchronously in the presence of glycerol as sole carbon source, the respiration rate showed two abrupt increases at about 0.45 and 0.95 of the cell-cycle and remained constant in the periods between successive rises. 3. Catalase, succinate dehydrogenase, NADH-cytochrome c oxidoreductase and acid p-nitrophenyl-phosphatase all showed peak patterns of expression in synchronous cultures. 4. Cytochrome c oxidase and cytochromes a+a(3) both showed step patterns of expression with two rises per cell-cycle. 5. Cytochromes c(548), b(554) and b(560) all followed similar time-courses in step patterns of expression, but these were distinct from, and more complex than, that of cytochromes a+a(3). 6. These results are compared with those previously obtained with glucose-grown cultures, and the part played by catabolite repression in the expression of respiratory activities in the cell-cycle is assessed.

Oxygen Uptake and Mitochondrial Enzyme Activities in the Mitotic Cycle of Physarum polycephalum

“…While periodicity in the accumulation of respiratory enzymes, including cytochrome oxidase, has been reported in a number of synchronous micro-organisms, the pattern has not previously been found to coincide with that of O2 uptake (Cottrell & Avers, 1970;Forde & John, 1973;Poole & Lloyd, 1974). It is therefore unlikely that fluctuations in the specific activity of the terminal oxidase can account for the respiratory oscillations observed here.…”

The Influence of Growth Substrate and Capacity for Oxidative Phosphorylation on Respiratory Oscillations in Synchronous Cultures of Escherichia coli K12

“…(i) During the cell cycle of cells grown with glucose, the respiratory activity oscillates with a periodicity of half a cycle (Poole et al, 1973). (ii) Growth in the presence of a non-catabolite-repressive carbon source, glycerol, results in stepwise increases in O2 uptake (Poole & Lloyd, 1974). (iii) Oscillations observed with glucose as carbon source do not appear to be due to respiratory control in vivo, i.e.…”

Mitochondrial adenosine triphosphatase of the fission yeast, Schizosaccharomyces pombe 972h-. Changes in activity and inhibitor-sensitivity in response to catabolite repression

“…succinate dehydrogenase) occurs during exponential growth on glucose when the concentration of the carbon source falls to below 35mM; cytochrome c oxidase is de-repressed at 15 mM-glucose . The catabolite de-repressed state may also be studied in glycerol-grown cells (Poole & Lloyd, 1974). In the present study we report that complex changes in specific activities of mitochondrial ATPase, and in its sensitivity to nine different inhibitors, occur during glucose de-repression and are correlated with the activity of a naturally occurring ATPase inhibitor.…”

“…approx. 40mM) when growth ceases (Poole & Lloyd, 1974). The specific activity of ATPase is high in the midexponential growth phase, about twice that ofglucosegrown cells at the same stage ( Fig.…”

“…Maintenance, growth and harvesting ofthe organism S. pombe 972h-was maintained on 3 % (w/v) maltextract agar and grown with forced aeration in minimal media containing either 1 % glucose or 1 % glycerol, exactly as described previously Poole & Lloyd, 1974). Organisms were counted in a Thoma haemocytometer slide (Hawkesley, Lancing, Sussex, U.K.).…”