Changes in rhizosphere bacterial communities during remediation of heavy metal-accumulating plants around the Xikuangshan mine in southern China

Guo, Dongchu; Fan, Zhouzhou; Lu, Shuyu; Ma, Yongjiao; Nie, Xiaohong; Tong, Fangping; Peng, Xiawei

doi:10.1038/s41598-018-38360-2

Cited by 70 publications

(37 citation statements)

References 52 publications

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“…In order to shed light on these processes and to assess variations caused by Zn soil addition and/or CMP amendment, we analyzed the rhizosphere microbiome of N12 saplings because it may contribute to plant growth, health (Richter-Heitmann et al, 2016) and metal tolerance. Our interest comes from several studies which have clearly established that the rhizosphere corresponds to the plant-soil compartment harboring the highest microbe richness diversity (Guo et al, 2019;Latini et al, 2019;Brereton et al, 2020). Soil rhizospheres are very complex matrices in relation to their bacterial and fungal communities, which can be readily analyzed in terms of diversity and composition, by deep sequencing (Burges et al, 2017;Fan et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Effects of Zinc Pollution and Compost Amendment on the Root Microbiome of a Metal Tolerant Poplar Clone

et al. 2020

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Until recently, many phytoremediation studies were focused solely on a plants ability to reclaim heavy metal (HM) polluted soil through a range of different processes, such as phytoextraction and phytostabilization. However, the interaction between plants and their own rhizosphere microbiome represents a new research frontier for phytoremediation. Our hypothesis is that rhizomicrobiome might play a key role in plant wellness and in the response to external stimuli; therefore, this study aimed to shed light the rhizomicrobiome dynamics after an organic amendment (e.g., compost) and/or HM pollution (e.g., Zn), and its relation with plant reclamation ability. To reach this goal we set up a greenhouse experiment cultivating in pot an elite black poplar clone (N12) selected in the past for its excellent ability to reclaim heavy metals. N12 saplings were grown on a soil amended with compost and/or spiked with high Zn doses. At the end of the experiment, we observed that the compost amendment strongly increased the foliar size but did not affect significantly the Zn accumulation in plant. Furthermore, the rhizomicrobiome communities (bacteria and fungi), investigated through NGS, highlighted how α diversity increased in all treatments compared to the untreated N12 saplings. Soil compost amendment, as well as Zn pollution, strongly modified the bacterial rhizomicrobiome structure. Conversely, the variation of the fungal rhizomicrobiome was only marginally affected by soil Zn addition, and only partially impaired by compost. Nevertheless, substantial alterations of the fungal community were due to both compost and Zn. Together, our experimental results revealed that organic amendment increased the bacterial resistance to external stimuli whilst, in the case of fungi, the amendment made the fungi microbiome more susceptible. Finally, the greater microbiome biodiversity does not imply, in this case, a better plant wellness or phytoremediation ability, although the microbiome plays a role in the external stimuli response supporting plant life.

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Section: Discussionmentioning

confidence: 99%

Effects of Zinc Pollution and Compost Amendment on the Root Microbiome of a Metal Tolerant Poplar Clone

et al. 2020

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“…Additionally, Granulicatella-whose relative abundance has been negatively associated with dental caries 16 -decreased in abundance with higher levels of antimony. A search for microbiome studies of antimony found only 14 studies (all in soil or water) [17][18][19][20][21][22][23][24][25][26][27][28][29][30] .…”

Section: Discussionmentioning

confidence: 99%

Low levels of salivary metals, oral microbiome composition and dental decay

Davis

Bakulski

Goodrich

et al. 2020

Sci Rep

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Salivary microbiome composition can change following exposure to environmental toxicants, e.g., heavy metals. We hypothesized that levels of salivary nutrients and metals would correlate with salivary microbiome composition and be associated with dental decay. Here we assess the salivary concentrations of 5 essential minerals (cobalt, copper, manganese, molybdenum, and zinc), 4 metals with some evidence of normal physiological function (chromium, nickel, tungsten, and vanadium), and 12 with known toxicity (antimony, arsenic, barium, beryllium, cadmium, cesium, lead, mercury, platinum, thallium, tin, and uranium), and their associations with salivary microbiome composition and dental decay in 61 children and adults. 16 metals were detected in 54% of participants; 8 were found in all. Marked differences in salivary bacterial taxa were associated with levels of antimony, arsenic, and mercury, after adjusting for multiple testing. further, antimony levels were associated with the presence of decayed teeth. thus, salivary metal levels, even at low concentrations, may impact oral health. The salivary microbiome provides a window into the overall health of the body and likely changes in response to environmental exposures, such as metals. Like humans, bacteria require certain metals to carry out metabolic processes, and often compete with host cells to take up these metals through transporters and other acquisition systems 1,2. However, toxicity is a concern for bacteria, as some metals-such as cadmium and copper-are bactericidal at certain concentrations 3. Further, even essential metals can be toxic, depending on the dose (Supplemental Tables S1-S3). Therefore, we hypothesized that there will be changes in the salivary microbiome composition in response to salivary metal levels which may directly affect oral health. Dental caries is the most common chronic disease in children aged 6-19 4 and periodontal disease is the most common cause of adult tooth loss 5. The oral microbiome is an important determinant of both dental caries and periodontal disease. As it is now clear that the microbiome at all body sites responds to environmental stressors such as pathogens and environmental chemicals, it is critical to understand the implications of metal exposures on the structure, composition, and function of the oral microbiome, taking into account whether the metal is exclusively toxic, an essential mineral, or has some physiological functions. When certain toxic metals, e.g., lead or cadmium, are measured in blood or urine the resulting levels can be compared to guidelines for concentrations of concern 6 ; however, no guidelines exist for salivary metal levels. Saliva biomarker exposure assessment could be particularly useful in studies where the oral cavity is the primary target region of toxicity (e.g., studies of the oral microbiome or dental health). Some studies have assessed salivary metals, but the number of publications and the scope of metals assessed are limited. Lead 7,8 and mercury 9 have been of particular intere...

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“…8). Previous study has shown bacterial community structure were in uenced by pH, Cr, Sb, As, Zn and moisture content, but pH was the most dominant factor 37 .…”

Section: Analysis Of the Relationship Between Environmental Factors And The Rhizospheric Microbial Communitymentioning

confidence: 90%

Different Soil Salinity Imparts Clear Alteration In Rhizospheric Bacterial Community Dynamics In Rice And Peanut.

Islam

Bhattacharya

Sarkar

et al. 2021

Preprint

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The rhizospheric microbiome is capable of changing the physio-chemical properties of its own micro-environment and found to be indispensable in overall health of the host-plant. The interplay between the rhizospheric environment and the microbiota residing therein tune the physiology of the associated plant. In this study, we have determined how the soil properties and the host-plant remains as an important parameter for microbial community-dynamics in the rhizosphere of rice and peanut. In addition to check the physio-chemical parameters of the rhizospheric soil, we have also prepared the metagenomic DNA from each rhizospheric soil followed by high-throughput sequencing and sequence-analysis to predict the OTUs that represents the community structure. The alpha-diversity of the bacterial community in the RRN sample was highest, while the lowest was in PRS sample. Actinobacteria is the most predominant phylum in PRN, PRS and RRN whereas Acidobacteria in RRS. We found a clear shift in bacterial community over the rice and peanut rhizosphere and also over these host-rhizospheres from normal and high saline region. The rhizospheric bacterial community composition found to be affected by the close-by environmental factors. Thus, the rhizospheric bacterial community-structure is related to both the adjoining soil characters and the type of the hosts.

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Changes in rhizosphere bacterial communities during remediation of heavy metal-accumulating plants around the Xikuangshan mine in southern China

Cited by 70 publications

References 52 publications

Effects of Zinc Pollution and Compost Amendment on the Root Microbiome of a Metal Tolerant Poplar Clone

Effects of Zinc Pollution and Compost Amendment on the Root Microbiome of a Metal Tolerant Poplar Clone

Low levels of salivary metals, oral microbiome composition and dental decay

Different Soil Salinity Imparts Clear Alteration In Rhizospheric Bacterial Community Dynamics In Rice And Peanut.

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