Changes in serum and synovial fluid biomarkers after acute injury (NCT00332254)

Catterall, J.; Stäbler, Thomas; Flannery, Carl R.; Kraus, Virginia B.

doi:10.1186/ar3216

Cited by 236 publications

(254 citation statements)

References 47 publications

Supporting

Mentioning

224

Contrasting

Unclassified

Order By: Relevance

“…Finally, we do not have long-term joint aspirations or patient followup to demonstrate if these proteases continue to be present in injured joints and/or lead to posttraumatic OA. .We did not compare our aspiration results with serum results, which has been done in other studies because these studies failed to show good correlations between serum and synovial fluid concentrations [7,14,16]. Also it is possible that our dilution methodology, especially when aspirating joint fluid from a normal joint, could have altered the true levels of degradative products and enzymes in our study.…”

Section: Discussionmentioning

confidence: 89%

“…In an effort to look specifically at the presence of proteolytic enzymes after traumatic injury, several authors have shown elevated MMPs and aggrecan degradation after ACL tear. However, the time from ACL injury to joint aspiration had considerable variability in these studies [5,7,26,30]. There are currently no human studies of which we are aware that quantify the concentrations of MMPs and/or aggrecan degradation acutely after articular fracture.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Intraarticular Matrix Metalloproteinases and Aggrecan Degradation Are Elevated After Articular Fracture

Haller

Swearingen

Partridge

et al. 2015

Clin Orthop Relat Res

View full text Add to dashboard Cite

Background Posttraumatic osteoarthritis (OA) is a variant of OA that can develop after articular injury. Although the mechanism(s) of posttraumatic OA are uncertain, the presence and impact of postinjury proteolytic enzymes on articular cartilage remain unknown. To our knowledge, there are no studies that evaluate the presence of matrix metalloproteinases (MMPs) or aggrecan degradation after articular fracture.Questions/purposes (1) Are MMP concentrations and aggrecan degradation elevated after intraarticular fracture? (2) Are MMP concentrations and aggrecan degradation greater in high-energy injuries compared with low-energy injuries? (3) Do the concentrations of these biomarkers remain elevated at a secondary aspiration? Methods Between December 2011 and June 2013, we prospectively enrolled patients older than 18 years of age with acute tibial plateau fracture. Exclusion criteria included age older than 60 years, preexisting knee OA, injury greater than 24 hours before evaluation, contralateral knee injury, history of autoimmune disease, open fracture, and non-English-speaking patients. During the enrollment period, we enrolled 45 of the 91 (49%) tibial plateau fractures treated at our facility. Knee synovial fluid aspirations were obtained from both the injured and uninjured knees; two patients received aspirations in the emergency department and the remaining patients received aspirations in the operating room. Twenty patients who underwent spanning external fixator followed by definitive fixation were aspirated during both surgical procedures. MMP-1, -2, -3, -7, -9, -10, -12, and -13 concentrations were quantified using multiplex assays. Aggrecan degradation was quantified using sandwich enzyme-linked immunosorbent assay. Results There were higher concentrations of MMP-1 (3.89 ng/mL [95% confidence interval {CI}, 2.37-6.37] versus 0.37 ng/mL [95% CI, 0.23-0.61], p \ 0.001), MMP-3The institution of one or more of the authors (JMH, TFH) has received peer-reviewed research grant funding from the Orthopedic Trauma Association, LS Peery Foundation, and AO North America for this project. One of the authors certifies that he (TFH), or a member of his immediate family, has signed an agreement with DePuy Synthes (Warsaw, IN, USA) for consulting activities not related to the current study, and has not received payments or benefits, during the study period. One or more of the authors (CAS, KT) are employed at Eli Lilly (Indianapolis, IN, USA). All ICMJE Conflict of Interest Forms for authors and Clinical Orthopaedics and Related Research1 editors and board members are on file with the publication and can be viewed on request. Clinical Orthopaedics and Related Research1 neither advocates nor endorses the use of any treatment, drug, or device. Readers are encouraged to always seek additional information, including FDAapproval status, of any drug or device prior to clinical use. Each author certifies that his or her institution approved or waived approval for the reporting of this investigation and that all inves...

show abstract

Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Intraarticular Matrix Metalloproteinases and Aggrecan Degradation Are Elevated After Articular Fracture

Haller

Swearingen

Partridge

et al. 2015

Clin Orthop Relat Res

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

“…Parvizi et al [26] reported a high correlation coefficient (r 2 = 0.72) between these two CRP assays in the setting of total joint arthroplasty while Catterall et al [6] reported a positive correlation in patients with acute knee trauma. Catterall et al [6] postulated that diffusion of serum CRP into the joint could be responsible for an elevated synovial fluid CRP when elevated systemically. Parvizi et al [26] applied this to PJI and theorized that synovial permeability caused by local inflammation may allow for high levels of serum CRP to diffuse into the joint, thus increasing synovial CRP.…”

Section: Discussionmentioning

confidence: 99%

Is Synovial C-reactive Protein a Useful Marker for Periprosthetic Joint Infection?

Tetreault

Wetters

Moric

et al. 2014

Clin Orthop Relat Res

102

View full text Add to dashboard Cite

Background Serum C-reactive protein (CRP) is a general marker of inflammation, and recent studies suggest that measurement of CRP in synovial fluid may be a more accurate method for diagnosing periprosthetic joint infection (PJI). Questions/purposes We aimed to (1) determine if there is a correlation between serum and synovial CRP values, (2) establish cutoff values for diagnosing infection based on serum and synovial CRP, and (3) compare the utility of measuring CRP in synovial fluid versus serum for the diagnosis of PJI using standard assay equipment available at most hospitals. Methods Between February 2011 and March 2012, we invited all 150 patients scheduled for revision TKA (84) or THA (66) to participate in this prospective study, of whom 100% agreed. Data ultimately were missing for 31 patients, leaving 60 patients undergoing revision TKA and 59 undergoing revision THA (71% and 89% of the original group, respectively) for whom CRP level was measured in serum and synovial fluid samples. Patients were deemed to have a PJI (32) or no infection (87) using Musculoskeletal Infection Society criteria. Serum and synovial CRP levels were assayed using the same immunospectrophotometer and the correlation coefficient was calculated. Receiver operating characteristic curve analyses were performed to compare utility in diagnosing PJI, which included area under the curve, diagnostic threshold, and test sensitivity, specificity, predictive values, and accuracy. In 22 of 150 patients (14.7%), synovial CRP could not be measured because the sample was too viscous or hemolyzed. Results In the analyzed 119 samples, there was a strong correlation (r = 0.76; p \ 0.001) between synovial and serum CRP. The area under the curve was 0.90 both for the synovial fluid (95% CI, 0.82-0.97) and serum (95% CI, 0.84-0.96) CRP assays. The diagnostic thresholds were 6.6 mg/L for synovial fluid and 11.2 mg/L for serum. Sensitivities, specificities, positive predictive value, negative predictive value, and accuracies were similar for synovial fluid and serum assays. Conclusions Although recent studies have suggested a superiority of synovial fluid CRP over serum CRP for the diagnosis of PJI, we found that measurement of CRP in synovial fluid rather than serum using readily available

show abstract

“…Kulmala et al (2012) (GRAUW et al, 2009;CONNOLLY et al, 2012;CATTERALL et al, 2010 Em estudos com artrite reumatoide em humanos, foram encontradas citocinas com poder anti-inflamatório na articulação, são elas interleucina 10 (IL-10), interleucina 4 (IL-4) e interleucina 13 (IL-13). Acredita-se que a IL-10 iniba a liberação dos fatores pró-inflamatórios comentados acima.…”

Section: Fisiologia Do Ambiente Articularunclassified

Desenvolvimento de protocolo de reabilitação no período pós-operatório inicial de artroscopia em equinos

Stievani¹,

Silva²

View full text Add to dashboard Cite

Changes in serum and synovial fluid biomarkers after acute injury (NCT00332254)

Cited by 236 publications

References 47 publications

Intraarticular Matrix Metalloproteinases and Aggrecan Degradation Are Elevated After Articular Fracture

Intraarticular Matrix Metalloproteinases and Aggrecan Degradation Are Elevated After Articular Fracture

Is Synovial C-reactive Protein a Useful Marker for Periprosthetic Joint Infection?

Desenvolvimento de protocolo de reabilitação no período pós-operatório inicial de artroscopia em equinos

Contact Info

Product

Resources

About