Changes in Serum IgG Glycosylation Patterns for Abdominal Aortic Aneurysm Patients

Abstract

Background: B cells and autoantibodies play an important role in the pathogenesis of abdominal aortic aneurysm (AAA). IgG glycosylations are highly valued as potential disease biomarkers and therapeutic targets. Methods: Lectin microarray was applied to analyze the expression profile of serum IgG glycosylation in 75 patients with AAA, 68 autoimmune disease controls, and 100 healthy controls. Lectin blots were performed to validate the differences. The clinical relevance of lectins binding from the microarray results was explored in AAA patients. Results: Significantly lower binding level of SBA (preferred GalNAc) was observed for the AAA group compared with DCs (p < 0.001) and HCs (p = 0.049). A significantly lower binding level of ConA (preferred mannose) was observed in patients with aneurysm diameter >5 cm. Significantly higher binding of CSA (preferred GalNAc) was present for dyslipidemia patients, whereas a lower binding level of AAL (preferred fucose) was observed for hypertensive patients. Patients with diabetes had lower binding levels of IRA (preferred GalNAc) and HPA (preferred GalNAc) compared with those not with DM. PTL-L (R = 0.36, p = 0.0015, preferred GalNAc) was positively associated with aneurysm diameters, whereas DSL (R = 0.28, p = 0.014, preferred (GlcNAc)2-4) was positively associated with patients’ age. Symptomatic patients had a lower binding level of ConA (p = 0.032), and patients with coronary heart disease had higher binding levels of STL (p = 0.0029, preferred GlcNAc). Patients with ILT bound less with black bean crude (p = 0.04, preferred GalNAc). Conclusions: AAA was associated with a decreased IgG binding level of SBA (recognizing glycan GalNAc). Symptomatic patients with aneurysm <5 cm had a higher binding level of ConA (preferred mannose). Coronary heart disease and elder age were associated with increased IgG bisecting GlcNAc. IgG O-glycosylation (GalNAc) may play an important role in AAA pathogenesis and progression.

Changes in serum immunoglobulin G glycosylation patterns for antiphospholipid syndrome patients with lectin microarray

“…To detect the glycan profile of serum IgG in 413 participants, a commercial lectin microarray (BCBIO Biotech, Guangzhou, China) with 56 lectins was used, the reliability of which had been proven in previous studies 12‐14 . First, lectin microarrays were taken from −80°C, placed at room temperature for 1.5 h and incubated with a blocking buffer composed of 3% bovine albumin (BSA) in phosphate‐buffered saline (PBS) for 2 h. Then, microarrays were washed with phosphate‐buffered saline Tween (PBST) three times and incubated with 200 μL of 1:1000 diluted serum samples at 4°C overnight.…”

“…To detect the glycan profile of serum IgG in 413 participants, a commercial lectin microarray (BCBIO Biotech, Guangzhou, China) with 56 lectins was used, the reliability of which had been proven in previous studies. [12][13][14] First, lectin microarrays were taken from −80°C, placed at room temperature for 1.5 h and incubated with a blocking buffer composed of 3% bovine albumin (BSA) in phosphate-buffered saline (PBS) for 2 h. Then, microarrays were washed with phosphate-buffered saline Tween (PBST) three times and incubated with 200 μL of 1:1000 diluted serum samples at 4°C overnight. After washing the microarrays three times with PBST, 5 mL of 1:1000 diluted Cy3-labelled goat anti-human IgG antibodies (Jackson Immuno Research Labs, Pennsylvania, PA, USA) were added to the microarrays, then incubated at room temperature away from direct sunlight for an hour.…”

Retrospective screening of serum IgG glycosylation biomarker for primary Sjögren’s syndrome using lectin microarray

“…The detailed glycan binding specificities and type of linkage for each lectin could be found in Supplemental File . Detailed procedure could be seen in our previous works ( Hu et al, 2020 ; Li et al, 2022a ; Li et al, 2022b ; Zeng et al, 2021 ). Briefly, lectin microarrays were taken out from −80 °C and warmed up at room temperature for half an hour, then they were incubated with a blocking buffer (3% BSA in PBS) at room temperature for 2 h. After washing three times with PBS, 200 μl of 1:1,000 diluted samples serum was added and incubated with the microarrays at 4 °C overnight.…”

Aneurysm Rupture Prediction Based on Strain Energy-CFD Modelling

“…Abdominal aortic aneurysm (AAA) is a common form of cardiovascular disease for which early diagnosis is critical. Progression of the disease can lead to aneurysmal rupture, which has a high mortality rate due to catastrophic blood loss [1][2][3][4][5][6][7]. In an AAA, the descending aorta becomes enlarged, with the enlarged segment sometimes extending within the abdomen up to the bifurcation region.…”