Changes in sodium, calcium and magnesium ion concentrations in sturgeon (Huso huso) urine and in kidney morphology

Krayushkina, L. S.; Панов, А. А.; Gerasimov, Andrey; Potts, W. T. W.

doi:10.1007/bf00387513

Cited by 19 publications

(13 citation statements)

References 7 publications

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“…Although a good amount of information is now available on anadromous fish osmoregulation (review in Evans 1993 and Evans, Piermarini & Choe 2005), not much work has been done on osmoregulation potentials of sturgeons. However, the limited work indicated that mature sturgeons are generally capable of regulating their blood serum osmolarity and ionic concentration (Krayushkina, Panov, Grasimov & Potts 1996; Krayushkina & Semenova 2004). Juvenile sturgeons in the natural environment may spend a period of several days or years in the river to adapt their body osmosis to the surroundings before migration (Ginsburg 1975; Krayushkina & Semenova 2004).…”

Section: Introductionmentioning

confidence: 99%

Immunolocalization of Na⁺, K⁺-ATPase-rich cells in the gill and urinary system of Persian sturgeon,Acipenser persicus, fry

Khodabandeh

Khoshnood

Mosafer

2009

Aquaculture Research

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Localization of Na 1 , K 1 -ATPase-rich cells in the gill and urinary system of Acipenser persicus fry was performed through immuno£uorescence light microscopy using a mouse monoclonal antibody IgGa 5 raised against the a-subunit of chicken Na 1 , K 1 -ATPase. Di¡erent types of epithelia were clearly identi¢ed in the gill epithelium: epithelia of branchial arch, interbranchial septum, ¢lament and lamellar epithelium. The Na 1 , K 1 -ATPase-rich cells were found in the epithelia of branchial arch, interbranchial septum, ¢lament, interlamellar region and also in the lamellae. Histologically, the urinary system is divided into head kidney, trunk kidney and short caudal kidney. The head kidney is composed of the pronephric tubules and the haemopoietic tissues, while the trunk kidney is composed of a large number of glomeruli and convoluted nephrons. Each nephron consisted of a large glomerulus and tubules (neck, proximal, distal and collecting tubules) which connected to ureters. Posteriorly, ureters extended and joined together to form a small urinary bladder. In the urinary system, no speci¢c £uorescence staining was observed in the glomerulus, neck segment and proximal tubules. The distal tubule cells and collecting tubule cells showed a strong immunostaining of Na 1 , K 1 -AT-Pase. Epithelia of ureters and urinary bladder also showed several isolated immuno£uorescent cells. Immuno£uorescent cells were rich in Na 1 , K 1 -ATPase enzyme which is very important for osmoregulation.

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Section: Introductionmentioning

confidence: 99%

Immunolocalization of Na⁺, K⁺-ATPase-rich cells in the gill and urinary system of Persian sturgeon,Acipenser persicus, fry

Khodabandeh

Khoshnood

Mosafer

2009

Aquaculture Research

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“…It was possible to suppose that the sturgeons from different groups have specific adaptive mechanisms of osmotic and ionic regulation in accordance with the ecology of a species. The specific features of osmotic and ionic regulation in the sturgeons from these different groups are investigated to great extent (Krayushkina, 1974(Krayushkina, , 1983a(Krayushkina, , 1998Krayushkina, Moiseenko, 1977;Krayushkina et al, 1996Krayushkina et al, , 2001. The analysis of these investigations could form a true noting of the evolution of mechanism of osmotic and ionic regulation in acipenserids, the trend of physiological-biochemical changes in this mechanism and could emphasize the importance of a salinity as an ecological factor in the development of the level of osmotic and ionic homeostasis in acipenserids.…”

Section: Introductionmentioning

confidence: 99%

Considerations on evolutionary mechanisms of osmotic and ionic regulation in Acipenseridae: an overview

Krayushkina¹

2006

J Appl Ichthyol

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The changes in osmolarity and ion concentrations of blood serum, urine and intestinal liquid in different acipenserid species were studied during their acclimation to sea water, identifying the osmotic and ionic regulation involved in these species. The freshwater species (Acipenser ruthenus, A. baerii from Baikal Lake) can adapt to brackish water of 12.5‰ salinity as a conformer. The freshwater species Pseudoscaphyrhinchus kaufmanni is also an osmo-conformer, but tolerates brackish water up to 10‰ salinity only. Freshwater species having short migrations to the estuary for feeding (A. baerii from Lena River) have limited capacity in osmotic and ionic regulation. The diadromous brackishwater species (A. gueldenstaedtii, A. stellatus, Huso huso) held in brackish water of 12.5-12.7‰ salinity and diadromous seawater species (A. brevirostrum and A. oxyrhynchus) held in sea water of up to 30.5‰ are capable to support blood serum osmolarity at relative constant levels during the transition phase from hyper-to hypo-osmotic regulation after the transition from fresh water. However functional level of the mechanism of osmotic and ionic homeostasis is not equal in different species. The higher the medium salinity (where species live in the nature) the higher the functional level of these mechanisms. This level rises in a number of acipenserids which rank as follow "Pseudoscaphyrhinchus kaufmanni ? A. ruthenus and A. baerii from Baikal Lake ? A. baerii from Lena River? A. gueldenstaedtii? Huso huso? A. stellatus? A. brevirostrum? A. oxyrhynchus". The development of osmotic and ionic homeostasis is connected with the evolution of kidney function (the increase of sodium re-absorption and magnesium secretory activities), with the strengthening sodium absorption function of the intestines, with the strengthening of ionic secretory function of the gills and the changes regulatory function of endocrine system.

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“…The presence of two segments in the PT is irregular in the acipenserids (Gambaryan, 1988) as in other fishes. Only one segment was reported in the P. spathula (Krayushkina et al , 2000) and in the great sturgeon Huso huso (L. 1758) (Krayushkina et al , 1996 a ), but two segments were observed in several adult sturgeons (Gambaryan, 1988; Stoskopf, 1993; Krayushkina et al , 2000), and in A. persicus fry (Khodabandeh et al , 2009 a ). As stated by Krayushkina et al (2000)‘the presence of second proximal tubule (PT2) could be a distinctive feature of anadromous marine sturgeon,’ if the presence of PT2 is related to the migratory status of the species, more comparative studies on different species are needed to reach a definitive answer.…”

Section: Discussionmentioning

confidence: 99%

“…Similar observations were made in the P. spathula (Krayushkina et al , 2000). In H. huso , Krayushkina et al (1996 a ) delimited two parts in the DT, which the features of DT2 corresponding to what was named here the collecting tubules. In this study, the NKA antibody strongly stained the entire cells and not only the basal part as in proximal cells, a feature which demonstrates the high capacities of the DT of A. persicus larvae in ion reabsorption in fresh water.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructure and osmoregulatory function of the kidney in larvae of the Persian sturgeon Acipenser persicus

Abadi

Khodabandeh

Behrouz

et al. 2011

Journal of Fish Biology

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The localization of Na(+) , K(+) -ATPase (NKA) and the ultrastructural features of kidney were examined in larvae of the Persian sturgeon Acipenser persicus (L 31-41 mm total length and 182·3-417·3 mg). Investigations were conducted through light and electron microscopy and through immunofluorescence for NKA detection. The kidney nephrons consisted of a large glomerulus and tubules (neck, proximal, distal and collecting), which connected to the ureters. Posteriorly, ureters extended and joined together into a thin-walled ureter terminal sac. Ultrastructurally, the glomerular cells (podocytes) possessed distinctive pedicels that extended to the basal membrane. The proximal tubule (PT) showed two different cells. The cells lining the anterior part of PT possessed apical tall microvilli (c. 2·7 µm), a sub-apical tubular system, a basal nucleus and dense granules. Posteriorly in the cells, the sub-apical tubular system and granules were absent and round mitochondria associated with basolateral infoldings were found; the apical microvilli were reduced. Distal and collecting tubular cells showed the typical features of osmoregulatory cells, i.e. well-developed basolateral infoldings associated with numerous mitochondria. No immunofluorescence of NKA was detected in the glomeruli. A weak immunostaining was observed at the basolateral side of the cells lining the neck and PT. A strong immunostaining of NKA was observed in the entire cells of the distal tubules, collecting tubules and in some isolated cells of the ureters. In all immunostained cells, the basolateral region showed a much higher fluorescence and nuclei were immunonegative. In conclusion, the epithelial cells of kidney tubules had morphological and enzymatic features of ionocytes, particularly in the distal and collecting tubules. Thus, the kidney of A. persicus larvae possesses active ion exchange capabilities and, beside its implication in excretion, participates in osmoregulation.

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Changes in sodium, calcium and magnesium ion concentrations in sturgeon (Huso huso) urine and in kidney morphology

Cited by 19 publications

References 7 publications

Immunolocalization of Na⁺, K⁺-ATPase-rich cells in the gill and urinary system of Persian sturgeon,Acipenser persicus, fry

Immunolocalization of Na⁺, K⁺-ATPase-rich cells in the gill and urinary system of Persian sturgeon,Acipenser persicus, fry

Considerations on evolutionary mechanisms of osmotic and ionic regulation in Acipenseridae: an overview

Ultrastructure and osmoregulatory function of the kidney in larvae of the Persian sturgeon Acipenser persicus

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Changes in sodium, calcium and magnesium ion concentrations in sturgeon (Huso huso) urine and in kidney morphology

Cited by 19 publications

References 7 publications

Immunolocalization of Na+, K+-ATPase-rich cells in the gill and urinary system of Persian sturgeon,Acipenser persicus, fry

Immunolocalization of Na+, K+-ATPase-rich cells in the gill and urinary system of Persian sturgeon,Acipenser persicus, fry

Considerations on evolutionary mechanisms of osmotic and ionic regulation in Acipenseridae: an overview

Ultrastructure and osmoregulatory function of the kidney in larvae of the Persian sturgeon Acipenser persicus

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Immunolocalization of Na⁺, K⁺-ATPase-rich cells in the gill and urinary system of Persian sturgeon,Acipenser persicus, fry

Immunolocalization of Na⁺, K⁺-ATPase-rich cells in the gill and urinary system of Persian sturgeon,Acipenser persicus, fry