“…DGGE, PLFA, t-RFLP) that limit fine scale investigations of biological invasions on environmental microbiome diversity and taxonomical composition. Among the studies using high-throughput amplicon-sequencing techniques, most did not find changes in microbiome diversity [12,14,17,18,21,25,27,28,30], few reported a decrease of microbial diversity in response to invasion [15,20,29], and fewer still reported an increase [13,19]. Thus, there is still little consensus on the effects of biological invasions on the diversity and taxonomical structure of the environmental microbiomes, both tied to the stability and function of microbial communities [31,32].…”