Changes in spatial collagen content and collagen network architecture in porcine articular cartilage during growth and maturation

Rieppo, Jarno; Hyttinen, Marko; Halmesmäki, Esa P.; Ruotsalainen, Heli; Vasara, Anna; Kiviranta, Ilkka; Jurvelin, Jukka S.; Helminen, Heikki J.

doi:10.1016/j.joca.2008.09.004

Cited by 122 publications

(137 citation statements)

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“…The data on porcine AC by Rieppo et al also shows some evidence for a valley in collagen density near the articular surface for the youngest age group, but not for the older age groups [21, figure 2a]. Similarly, we find strong evidence for a valley in collagen density in the youngest animals that disappears with increasing age (table 4, figure 5c).…”

Section: Discussionsupporting

confidence: 80%

“…Rieppo et al [21] looked at domestic pig AC from the femoral groove at 4 months, 11 months and 21 months of age. As in our study, they found that collagen density shows a maximum between the superficial and deep zone in the youngest age group, and that collagen density increases monotonically between the superficial and the deep zone for the oldest animals [21, figure 4a].…”

Section: Discussionmentioning

confidence: 99%

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Postnatal development of depth-dependent collagen density in ovine articular cartilage

et al. 2010

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BackgroundArticular cartilage (AC) is the layer of tissue that covers the articulating ends of the bones in diarthrodial joints. Adult AC is characterised by a depth-dependent composition and structure of the extracellular matrix that results in depth-dependent mechanical properties, important for the functions of adult AC. Collagen is the most abundant solid component and it affects the mechanical behaviour of AC. The current objective is to quantify the postnatal development of depth-dependent collagen density in sheep (Ovis aries) AC between birth and maturity. We use Fourier transform infra-red micro-spectroscopy to investigate collagen density in 48 sheep divided over ten sample points between birth (stillborn) and maturity (72 weeks). In each animal, we investigate six anatomical sites (caudal, distal and rostral locations at the medial and lateral side of the joint) in the distal metacarpus of a fore leg and a hind leg.ResultsCollagen density increases from birth to maturity up to our last sample point (72 weeks). Collagen density increases at the articular surface from 0.23 g/ml ± 0.06 g/ml (mean ± s.d., n = 48) at 0 weeks to 0.51 g/ml ± 0.10 g/ml (n = 46) at 72 weeks. Maximum collagen density in the deeper cartilage increases from 0.39 g/ml ± 0.08 g/ml (n = 48) at 0 weeks to 0.91 g/ml ± 0.13 g/ml (n = 46) at 72 weeks. Most collagen density profiles at 0 weeks (85%) show a valley, indicating a minimum, in collagen density near the articular surface. At 72 weeks, only 17% of the collagen density profiles show a valley in collagen density near the articular surface. The fraction of profiles with this valley stabilises at 36 weeks.ConclusionsCollagen density in articular cartilage increases in postnatal life with depth-dependent variation, and does not stabilize up to 72 weeks, the last sample point in our study. We find strong evidence for a valley in collagen densities near the articular surface that is present in the youngest animals, but that has disappeared in the oldest animals. We discuss that the retardance valley (as seen with polarised light microscopy) in perinatal animals reflects a decrease in collagen density, as well as a decrease in collagen fibril anisotropy.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Postnatal development of depth-dependent collagen density in ovine articular cartilage

et al. 2010

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“…Articular cartilage is a multiphasic tissue, the solid phase of which is composed predominately of a collagen (mainly type II) fibrillar network (around 10-22% by wet weight) enmeshing proteoglycan macromolecules (2-7% by wet weight) (Maroudas et al, 1969;Muir et al, 1970;Bayliss and Ali, 1978;Inerot et al, 1978;Maroudas et al, 1980;Zirn et al, 1984;Mow et al, 1992;Rieppo et al, 2009;Gannon et al, 2012). Previous studies have demonstrated that the application of dynamic compression (Huang et al, 2010;Bian et al, 2012;Thorpe et al, 2013) or hydrostatic pressure (Miyanishi et al, 2006;Wagner et al, 2008;Ogawa et al, 2009;Huang et al, 2010;Meyer et al, 2011;Correia et al, 2012;Liu et al, 2012;Steward et al, 2012;Vinardell et al, 2012a;Liu et al, 2013) can enhance the sGAG and collagen content as well as the mechanical functionality of cartilage constructs engineered using MSCs.…”

Section: Discussionmentioning

confidence: 99%

Cyclic hydrostatic pressure promotes a stable cartilage phenotype and enhances the functional development of cartilaginous grafts engineered using multipotent stromal cells isolated from bone marrow and infrapatellar fat pad

Carroll

Buckley

Kelly

2014

Journal of Biomechanics

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a b s t r a c tThe objective of this study was to investigate how joint specific biomechanical loading influences the functional development and phenotypic stability of cartilage grafts engineered in vitro using stem/progenitor cells isolated from different source tissues. Porcine bone marrow derived multipotent stromal cells (BMSCs) and infrapatellar fat pad derived multipotent stromal cells (FPSCs) were seeded in agarose hydrogels and cultured in chondrogenic medium, while simultaneously subjected to 10 MPa of cyclic hydrostatic pressure (HP). To mimic the endochondral phenotype observed in vivo with cartilaginous tissues engineered using BMSCs, the culture media was additionally supplemented with hypertrophic factors, while the loss of phenotype observed in vivo with FPSCs was induced by withdrawing transforming growth factor (TGF)-β3 from the media. The application of HP was found to enhance the functional development of cartilaginous tissues engineered using both BMSCs and FPSCs. In addition, HP was found to suppress calcification of tissues engineered using BMSCs cultured in chondrogenic conditions and acted to maintain a chondrogenic phenotype in cartilaginous grafts engineered using FPSCs. The results of this study point to the importance of in vivo specific mechanical cues for determining the terminal phenotype of chondrogenically primed multipotent stromal cells. Furthermore, demonstrating that stem or progenitor cells will appropriately differentiate in response to such biophysical cues might also be considered as an additional functional assay for evaluating their therapeutic potential.

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“…However, the molecular specificity of the univariate parameters for collagen and proteoglycans in human AC has been questioned because of the overlap between the absorption peaks (33). Enzymatic removal of proteoglycans can be used before conducting the measurements to increase the specificity of amide I parameter for collagen (34)(35)(36).…”

Section: ¡1mentioning

confidence: 99%

Vibrational spectroscopy of articular cartilage

Rieppo

Töyräs

Saarakkala

2016

Applied Spectroscopy Reviews

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Articular cartilage is a connective tissue that is located at the ends of long bones. Type II collagen, proteoglycans, water, and chondrocytes are the main constituents of articular cartilage. Osteoarthritis, the most common joint disease in the world, causes degenerative changes in articular cartilage tissue. Fourier transform infrared, Raman, and near infrared spectroscopic techniques offer versatile tools to assess biochemical composition and quality of articular cartilage. These vibrational spectroscopic techniques can be used to broaden our understanding about the compositional changes during osteoarthritis, and they also hold promise in disease diagnostics. In this article, the current literature of articular cartilage spectroscopic studies is reviewed.

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Changes in spatial collagen content and collagen network architecture in porcine articular cartilage during growth and maturation

Cited by 122 publications

References 40 publications

Postnatal development of depth-dependent collagen density in ovine articular cartilage

Postnatal development of depth-dependent collagen density in ovine articular cartilage

Cyclic hydrostatic pressure promotes a stable cartilage phenotype and enhances the functional development of cartilaginous grafts engineered using multipotent stromal cells isolated from bone marrow and infrapatellar fat pad

Vibrational spectroscopy of articular cartilage

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