Changes in Sugar Content during Cold Acclimation and Deacclimation of Cabbage Seedlings

Sasaki, Hidekazu

doi:10.1006/anbo.1996.0131

Cited by 113 publications

(74 citation statements)

References 17 publications

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“…This enhancement was explained in part by an inhibition of amylase activity under cold conditions. In cabbage seedlings, starch accumulated during cold acclimation and decreased during deacclimation (31). A comparison between the changes in chilling susceptibility of grapevine plantlets and their starch content indicates that starch may also play a role in protecting plant tissues against chilling.…”

Section: Vol 72 2006mentioning

confidence: 99%

Enhancement of Chilling Resistance of Inoculated Grapevine Plantlets with a Plant Growth-Promoting Rhizobacterium,Burkholderia phytofirmansStrain PsJN

Barka

Nowak

Clément

2006

Appl Environ Microbiol

506

246

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In vitro inoculation of Vitis vinifera L. cv. Chardonnay explants with a plant growth-promoting rhizobacterium, Burkholderia phytofirmans strain PsJN, increased grapevine growth and physiological activity at a low temperature. There was a relationship between endophytic bacterial colonization of the grapevine plantlets and their growth at both ambient (26°C) and low (4°C) temperatures and their sensitivities to chilling. The major benefits of bacterization were observed on root growth (11.8-and 10.7-fold increases at 26°C and 4°C, respectively) and plantlet biomass (6-and 2.2-fold increases at 26°C and 4°C, respectively). The inoculation with PsJN also significantly improved plantlet cold tolerance compared to that of the nonbacterized control. In nonchilled plantlets, bacterization enhanced CO 2 fixation and O 2 evolution 1.3 and 2.2 times, respectively. The nonbacterized controls were more sensitive to exposure to low temperatures than were the bacterized plantlets, as indicated by several measured parameters. Moreover, relative to the noninoculated controls, bacterized plantlets had significantly increased levels of starch, proline, and phenolics. These increases correlated with the enhancement of cold tolerance of the grapevine plantlets. In summary, B. phytofirmans strain PsJN inoculation stimulates grapevine growth and improves its ability to withstand cold stress.

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Section: Vol 72 2006mentioning

confidence: 99%

Enhancement of Chilling Resistance of Inoculated Grapevine Plantlets with a Plant Growth-Promoting Rhizobacterium,Burkholderia phytofirmansStrain PsJN

Barka

Nowak

Clément

2006

Appl Environ Microbiol

506

246

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show abstract

“…For the effect of low temperature, elevated sugar content has been reported in cabbage harvested in late autumn to winter (Yano et al, 1981). In addition, sugar content has been reported to increase in cabbages cultivated briefly at low temperatures (Koster and Lynch, 1992;Sasaki et al, 1996Sasaki et al, , 1998Suzuki et al, 1999). These findings show that glucose and fructose content increase in the leaves of cabbages grown using relatively less nitrogen fertilizer and low temperatures.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Small Cell Size on the Sweetness of Cabbage (Brassica oleracea L.) Leaves

Kano

2008

J. Japan. Soc. Hort. Sci.

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“…For many fruits, sugars are the most important determinant of their quality. Soluble carbohydrate also plays a role in stress tolerance, including tolerance to cold (Sasaki et al, 1996), drought (Keller and Ludlow, 1993), and salt (Sacher and Staples, 1985). The new method may be available to investigate the role of carbohydrates from these aspects.…”

Section: Discussionmentioning

confidence: 99%

“…To extract soluble carbohydrates, cell membranes should be decomposed. Immersing plant tissue in hot water has been shown to decompose cell membranes, leading to measurable increases in electroconductivity due to electrolytes leaked from plant cells (Sasaki et al, 1996). We supposed that a sufficient portion of soluble carbohydrate for analysis would leak from tissue by immersion in hot water or hot ethanol solution without requiring a homogenization step.…”

Section: Introductionmentioning

confidence: 99%

A Simple and Rapid Extraction Method of Carbohydrates from Petals or Sepals of Four Floricultural Plants for Determination of Their Content

Norikoshi¹,

Imanishi²,

Ichimura

2008

J. Japan. Soc. Hort. Sci.

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We developed a simple and rapid extraction method of soluble carbohydrates from petals for analysis by high performance liquid chromatography (HPLC), using a centrifugal filter device in a test tube without homogenization. Rose 'Sonia' petals were immersed in 99.5% ethanol solution in a test tube and kept at 75°C for 20 min. Sorbitol was then added to the solution as an internal standard, and the petals were transferred to the centrifugal filter device and centrifuged at 12000 × g for 10 min. Following removal of the filtrate, 99.5% ethanol was added to the filter device and a second round of centrifugation performed. Filtrated solution obtained from both centrifugations was combined with the ethanol solution remaining in the test tube, heated to dryness at 80°C, and used for HPLC analysis. Few differences in soluble carbohydrate content were observed between the new method and a conventional method in which soluble carbohydrates are extracted by homogenization. We confirmed that most carbohydrate was extracted by the new method. Moreover, the soluble carbohydrate content of samples extracted from 'Sonia' petals using the new method did not change during one week of storage at −30°C, indicating the stability of the samples. Marked differences in soluble carbohydrate content were not observed among 'Chanel', 'New Bridal', 'Rote Rose', or 'Saturn' rose petals, carnations or Tweedia caerulea petals or Delphinium sepals, using either the new or conventional method. Marked differences in soluble carbohydrate content were also not observed among leaves and stems in carnations, Delphinium or rose 'Sonia' using either the new or conventional method. These results suggest that the new method, which does not require homogenization, appears to be a more simple and rapid method of extraction of soluble carbohydrates from various organs of floricultural plants.

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Changes in Sugar Content during Cold Acclimation and Deacclimation of Cabbage Seedlings

Cited by 113 publications

References 17 publications

Enhancement of Chilling Resistance of Inoculated Grapevine Plantlets with a Plant Growth-Promoting Rhizobacterium,Burkholderia phytofirmansStrain PsJN

Enhancement of Chilling Resistance of Inoculated Grapevine Plantlets with a Plant Growth-Promoting Rhizobacterium,Burkholderia phytofirmansStrain PsJN

The Effect of Small Cell Size on the Sweetness of Cabbage (Brassica oleracea L.) Leaves

A Simple and Rapid Extraction Method of Carbohydrates from Petals or Sepals of Four Floricultural Plants for Determination of Their Content

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