Changes in the activities of ammonia assimilation enzymes during senescence of Brassica napus leaf protoplasts

Watanabe, Masami; Hanamoto, Tomoko; Watanabe, Yukio

doi:10.1007/978-94-009-0047-9_46

Cited by 2 publications

(1 citation statement)

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“…capitata was observed after the application of Put and Spd, while Spm was not effective. There have been no comparative studies in other species and the regeneration from protoplast cultures treated with PAs was not reported [51,60] or not achieved [55]; however, exogenous Put was reported to enhance plant regeneration from calli in Picea abies L. [61]. Spd was the most effective for shoot regeneration in Cucumis sativus L. [62] shot tip explants, or Withania somnifera L. [63] nodal Put is the precursor of Spd and Spm, and spermidine synthase (SPDS, EC 2.5.1.16) catalyzes Spd formation from Put [14].…”

Section: Regeneration From Polyamine-treated Protoplast Culturesmentioning

confidence: 99%

Exogenously Applied Polyamines Reduce Reactive Oxygen Species, Enhancing Cell Division and the Shoot Regeneration from Brassica oleracea L. var. capitata Protoplasts

Kiełkowska

Adamus

2021

Agronomy

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Polyamines (PAs) are organic molecules that are found in plants and animals. In plants, they are involved in the regulation of cellular growth, apoptosis, rooting, flower development, and stress responses. The effect of exogenously applied polyamines on the development of Brassica oleracea L. var. capitata protoplast cultures was studied. Protoplasts were isolated from hypocotyls of 2-week-old seedlings of three accessions and they were cultured in liquid media supplemented with putrescine (Put), spermidine (Spd), and spermine (Spm) at concentrations of 0 (control), 10, 20, and 40 µM. In the very early culture (24 and 48 h), cellular reactive oxygen species levels (ROS) in live cells were monitored using a fluorescent probe. The Put- and Spd-treated protoplasts exhibited lower fluorescence intensities, which corresponded to lower ROS accumulation as compared to the PA-free control. The protoplast viability was affected by the type of polyamine applied rather than its concentration. Put and Spd had a beneficial effect on the mitotic activity of the cultured cells, which was observed in all tested accessions. The highest frequency of shoot organogenesis (21%) was obtained from microcalli derived from the protoplasts cultured on the medium supplemented with 10 µM Put. Analysis of the ploidy level of the regenerants showed that the vast majority were diploids. Our results demonstrated that exogenously applied PAs maintained the viability of B. oleracea L. var. capitata protoplasts by alleviating oxidative stress and stimulating mitotic activity, which further affected the plant regeneration process.

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