Changes in the Antioxidant Potential of Nori Sheets During <i>in vitro</i> Digestion with Pepsin

Indumathi, Parimelazhagan; Mehta, Alka

doi:10.1080/10498850.2015.1125981

Cited by 8 publications

(3 citation statements)

References 30 publications

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“…Increases in antioxidant activity in SGID have also been reported for whey protein α-lactalbumin, which showed a considerable increase in ORAC value compared to the unhydrolyzed control [ 37 ]. A pepsin hydrolysate obtained from Nori sheets (Porphyra) also showed an increase in the DPPH scavenging ability up to 60% following proteolytic treatment compared to 30% by the unhydrolyzed algal extract [ 38 ].…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and chemoprotective peptides from simulated gastrointestinal digested (SGID) protein hydrolysate of Pyropia yezoensis against acetaminophen-induced HepG2 cells

Ulagesan

Eom

Nam

et al. 2022

Bioprocess Biosyst Eng

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Excessive production of reactive oxygen and nitrogen species may result in oxidative damage to tissues and organs. Oxidative stress is a pathological mechanism that contributes to the initiation and progression of liver injury. In the present study, antioxidative peptides purified from simulated gastrointestinal-digested (SGID) protein hydrolysate of Pyropia yezoensis , showed significant antioxidant activity and also showed a protective effect against acetaminophen (N-acetyl-p-aminophenol, APAP) -induced injury in HepG2 (human liver cancer cells) cells. The antioxidant activity was increased in a dose-dependent manner. Higher cell viability (73.26 ± 0.9%) and decreasing NO levels (107.6 ± 8.9%) were observed in 15 mM APAP-induced cells when treated with the concentration of (100 μg ml −1 ) Pyropia peptide. Py. (pep). The sequences of the eight identified peptides present in the active fractions of the protein hydrolysate included hydrophobic and aromatic amino acids, which may have been responsible for their chemoprotective and antioxidant activities. Results indicated that the treatment with the Pyropia—peptides significantly promoted the proliferation of HepG2 cells, protecting them against APAP-mediated injury, and showed a significant antioxidant capacity. This study revealed that the Py. (pep) will be beneficial in treating drug-induced oxidative stress and liver damage conditions. Py. (pep) can also serve as a better alternative for synthetic antioxidant drugs.

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Section: Resultsmentioning

confidence: 99%

Antioxidant and chemoprotective peptides from simulated gastrointestinal digested (SGID) protein hydrolysate of Pyropia yezoensis against acetaminophen-induced HepG2 cells

Ulagesan

Eom

Nam

et al. 2022

Bioprocess Biosyst Eng

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“…The enzymatic hydrolysis was performed according to previously described method. 8 The obtained hydrolysate was lyophilized (Micromodulyo freeze dryer, Thermo Scientific, USA) and stored at -80°C until further analysis.…”

Section: Preparation Of Enzymatic Hydrolysatementioning

confidence: 99%

“…7 Previously, anticoagulant and antioxidant studies of the enzymatic hydrolysate from P. yezoensis have been reported from our laboratory. 8,9 Immunomodulatory activities of Porphyran. 10 and Phycoerythrin.…”

Section: Introductionmentioning

confidence: 99%

Stimulation of Cell Mediated Immune Response by Protein Hydrolysate from Porphyra yezoensis

Venkatraman¹,

Syed²,

Mehta³

2021

IJPER

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Aim: To investigate the immunomodulatory effects of the protein hydrolysate from Porphyra yezoensis. Objectives: In this study, the in vitro immunomodulatory effects of protein hydrolysate of Porphyra yezoensis was studied by assay of phagocytosis and Nitric oxide production in RAW 264.7 macrophages and DTH (Delayed type hypersensitivity), NBT (Nitroblue tetrazolium) and Neutrophil adhesion assay in mice. Results: Hydrolysate treatment was found to be non-toxic in both in vitro and in vivo models. In vitro results suggested that hydrolysate could enhance the phagocytic activity and also inhibit the production of nitric oxide. Oral administration of hydrolysate (500 mg/kg) evoked an increase in the percent neutrophil adhesion to nylon fibers as well as potentiated the DTH reaction induced by SRBC (Sheep red blood cells). A phagocytic response was observed with significant changes in formation of formazan crystals. Conclusion: The results indicated that enzymatic hydrolysate from P. yezoensis could enhance the cell mediated immune response. Thus, the Nori protein hydrolysate could be explored as a possible natural immune stimulant to be used as nutraceuticals.

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Health Benefits and Pharmacological Effects of Porphyra Species

Venkatraman

Mehta

2018

Plant Foods Hum Nutr

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Changes in the Antioxidant Potential of Nori Sheets During in vitro Digestion with Pepsin

Cited by 8 publications

References 30 publications

Antioxidant and chemoprotective peptides from simulated gastrointestinal digested (SGID) protein hydrolysate of Pyropia yezoensis against acetaminophen-induced HepG2 cells

Antioxidant and chemoprotective peptides from simulated gastrointestinal digested (SGID) protein hydrolysate of Pyropia yezoensis against acetaminophen-induced HepG2 cells

Stimulation of Cell Mediated Immune Response by Protein Hydrolysate from Porphyra yezoensis

Health Benefits and Pharmacological Effects of Porphyra Species

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