Changes in the content of steroidal glycoalkaloids in potato tubers grown in the field and in the greenhouse under different conditions of light, temperature and daylength

Dimenstein, L; Lisker, N.; Kedar, N.; Levy, David A.

doi:10.1006/pmpp.1997.0098

Cited by 33 publications

(11 citation statements)

References 23 publications

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“…The increases in the concentrations of glykoalkaloids in plants exposed to HS agreed with the study by Coria et al [31], which showed that high α-solanidine content in potato tubers could be related to exposure to high temperature (immersion in hot water at 35°C for 4 h). Other studies, moreover, reported enhanced total glykoalkaloid levels in potato tubers after exposure to heat/drought [32,33].…”

Section: Heat Stressmentioning

confidence: 93%

Metabolomic analysis of wild and transgenic Nicotiana langsdorffii plants exposed to abiotic stresses: unraveling metabolic responses

et al. 2015

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Nicotiana langsdorffii plants, wild and transgenic for the Agrobacterium rhizogenes rol C gene and the rat glucocorticoid receptor (GR) gene, were exposed to different abiotic stresses (high temperature, water deficit, and high chromium concentrations). An untargeted metabolomic analysis was carried out in order to investigate the metabolic effects of the inserted genes in response to the applied stresses and to obtain a comprehensive profiling of metabolites induced during abiotic stresses. High-performance liquid chromatography separation (HPLC) coupled to high-resolution mass spectrometry (HRMS) enabled the identification of more than 200 metabolites, and statistical analysis highlighted the most relevant compounds for each plant treatment. The plants exposed to heat stress showed a unique set of induced secondary metabolites, some of which were known while others were not previously reported for this kind of stress; significant changes were observed especially in lipid composition. The role of trichome, as a protection against heat stress, is here suggested by the induction of both acylsugars and glykoalkaloids. Water deficit and Cr(VI) stresses resulted mainly in enhanced antioxidant (HCAs, polyamine) levels and in the damage of lipids, probably as a consequence of reactive oxygen species (ROS) production. Moreover, the ability of rol C expression to prevent oxidative burst was confirmed. The results highlighted a clear influence of GR modification on plant stress response, especially to water deficiency-a phenomenon whose applications should be further investigated. This study provides new insights into the field of system biology and demonstrates the importance of metabolomics in the study of plant functioning.

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Section: Heat Stressmentioning

confidence: 93%

Metabolomic analysis of wild and transgenic Nicotiana langsdorffii plants exposed to abiotic stresses: unraveling metabolic responses

et al. 2015

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“…Moreover, SGA content dramatically decreased concomitantly with an increase in phytoalexins following inoculation of tuber slices with fungi or treatment with elicitors of fungal origin. However, this was shown in the pith of the tubers and not in the peel, which is the first tissue to be invaded by pathogens (reviewed by Dimenstein et al, 1997). Potatoes also contain other proteins and metabolites that may protect the tuber against attack from insects and microorganisms (reviewed by Friedman, 2006).…”

Section: Glycoalkaloids General Characteristicsmentioning

confidence: 99%

Criteria for Safe Use of Plant Ingredients in Diets for Aquacultured Fish

Hemre

Amlund

Aursand

et al. 2018

EJNFS

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“…clones USDA 8380-1 (chc 80-1) and 55-1 (chc 55-1) (obtained from et al 1986), which accumulate high levels of SGA; S. phureja Juz.& Buk. clone BARD 1-3 (phu 1-3), which exhibits a low level of SGA; a hybrid of chc 80-1 and phu 1-3, CP-2 (obtained from Dr Veilleux R.E., Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA; Veilleux and Miller 1998;BouarteMedina et al 2002), and S. tuberosum L. cultivars LT-7 (a clone received from the International Potato Center, Lima, Peru; Dimenstein et al 1997) and Desirée. Plants were grown in pots Wlled with a perlite soil-less medium in a greenhouse under natural winter/spring conditions (average temperature range of 10-20°C).…”

Section: Plant Materialsmentioning

confidence: 99%

Potato steroidal glycoalkaloid levels and the expression of key isoprenoid metabolic genes

Krits

Fogelman

Ginzberg

2007

Planta

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The potato steroidal glycoalkaloids (SGA) are toxic secondary metabolites, and their total content in tubers should not exceed 20 mg/100 g fresh weight. The two major SGA in cultivated potato (Solanum tuberosum) are alpha-chaconine and alpha-solanine. SGA biosynthetic genes and the genetic factors that control their expression have not yet been determined. In the present study, potato genotypes exhibiting different levels of SGA content showed an association between high SGA levels in their leaves and tubers and high expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase 1 (hmg1) and squalene synthase 1 (pss1), genes of the mevalonic/isoprenoid pathway. Transcripts of other key enzymes of branches of the isoprenoid pathway, vetispiradiene/sesquiterpene synthase (pvs1) and sterol C24-methyltransferase type1 (smt1), were undetectable or exhibited stable expression regardless of SGA content, respectively, suggesting facilitated precursor flow to the SGA biosynthetic branch. The transcript ratio of solanidine glucosyltransferase (sgt2) to solanidine galactosyltransferase (sgt1) was correlated to the documented chaconine-to-solanine ratio in the tested genotypes. Significantly higher expression of hmg1, pss1, smt1, sgt1 and sgt2 was monitored in the tuber phelloderm than in the parenchyma of the tuber's flesh, targeting the former as the main SGA-producing tissue in the tuber, in agreement with the known high SGA content in the layers directly under the tuber skin.

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Changes in the content of steroidal glycoalkaloids in potato tubers grown in the field and in the greenhouse under different conditions of light, temperature and daylength

Cited by 33 publications

References 23 publications

Metabolomic analysis of wild and transgenic Nicotiana langsdorffii plants exposed to abiotic stresses: unraveling metabolic responses

Metabolomic analysis of wild and transgenic Nicotiana langsdorffii plants exposed to abiotic stresses: unraveling metabolic responses

Criteria for Safe Use of Plant Ingredients in Diets for Aquacultured Fish

Potato steroidal glycoalkaloid levels and the expression of key isoprenoid metabolic genes

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