Changes in the distribution of thymidine-3H labeled cells in the growing liver acinus of neonatal rats

LeBouton, A. V.; Marchand, R.

doi:10.1016/0012-1606(70)90138-7

Cited by 36 publications

(12 citation statements)

References 21 publications

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“…These phases of development probably are related to the gradual establishment of adult lobulation that occurs during the postnatal period. As was suggested by LeBouton and Marchand (1970) and by LeBouton (19741, the development may be caused by the drastic alteration, at birth, in the amount and composition of blood circulating through the liver. However, it is also possible that the development is due to an intrinsic nature of postnatally differentiating hepatocytes (Gumucio and Miller, 1982).…”

Section: Discussionmentioning

confidence: 90%

Fine structure and function of hepatocytes during development

Kanamura

Kanai

Watanabe

1990

J. Elec. Microsc. Tech.

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This paper reviews the fine structure and function of hepatocytes during fetal and postnatal development. Bile canaliculi develop to a mature appearance during perinatal and early postnatal periods, while bile secretory function is immature at birth and develops during the postnatal period. The rough endoplasmic reticulum is prominent and remains unchanged in amount during development, and the Golgi complex is large from early stages of fetal life. The smooth endoplasmic reticulum (SER) appears shortly before birth and increases in quantity to the adult level after birth. In mouse hepatocytes, Sv (area per unit cytoplasmic volume) of SER increases in perivenular cells between 1 and 10 days of age, although it remains low in periportal cells. Similarly, Sv of total ER increases in both periportal and perivenular cells between 1 and 5 days of age and then becomes greater in perivenular than periportal cells. This suggests that the postnatal increase in the drug-metabolizing capacity occurs predominantly in perivenular hepatocytes. SER proliferates after phenobarbital (PB) administration in both perivenular and periportal cells in 3-, 5-, and 10-day-old mice, and predominantly in perivenular cells in 20-day-old and adult mice. Thus the conspicuous proliferation of SER in perivenular hepatocytes after PB administration, characteristic of adult liver, becomes manifest during postnatal development. In mouse hepatocytes, Vv (volume per unit cytoplasmic volume) of mitochondrial matrix and peroxisomes and Sv of mitochondrial inner membrane and cristae increase in both periportal and perivenular cells between birth and 10 days of age. Then, Vv of mitochondrial matrix remains unchanged in periportal cells but decreases in perivenular cells. In general, the process of postnatal hepatocyte differentiation appears to include several phases of development; cell organelles develop during the early postnatal period, subsequently the cells undergo both functional and structural heterogeneity, and the late postnatal period after weaning is the time for a marked increase in cell size.

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Section: Discussionmentioning

confidence: 90%

Fine structure and function of hepatocytes during development

Kanamura

Kanai

Watanabe

1990

J. Elec. Microsc. Tech.

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“…Activities of glucose 6-phosphatase (Kanamura, 1975;Katz et al, 19761, ornithine carbamoyltransferase, succinate dehydrogenase, NADH dehydrogenase (Kanamura and Asada-Kubota, 19801, bile canalicular alkaline phosphatase (DeWolf-Peeters et al, 19721, and labeled hepatocytes after 3H-thymidine injection (LeBouton and Marchand, 1970) are evenly distributed throughout the acinus within a few days after birth. The zonal distribution of these enzyme activities and of cells synthesizing DNA, which is characteristic of adult liver, appears gradually during postnatal development.…”

Section: Discussionmentioning

confidence: 96%

“…It is likely that this change is related to the sequence of formation of adult lobulation that occurs during the postnatal period. LeBouton and Marchand (1970) and Le-Bouton (1974) suggested that postnatal change observed in the subacinar distribution of labeled nuclei after 3H-thymidine incorporation is caused by the drastic alteration at birth in the amount and composition of blood circulating through the liver. The fetal liver receives a relatively large volume of well-oxygenated blood rich in nutrients via the umbilical vein.…”

Section: Discussionmentioning

confidence: 99%

Development of morphologic heterogeneity of hepatocyte mitochondria in the mouse

et al. 1984

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The ultrastructure of mitochondria in periportal and perihepatic hepatocytes from newborn, 5-, 10-, and 20-day-old, and adult male ddY mice was analyzed by quantitative electron microscopy. In newborn and 5- and 10-day-old animals, the axial ratio (length per diameter), surface to volume ratio (area of the outer membrane per unit mitochondrial volume), and volume density were not significantly different between periportal and perihepatic cells. In 20-day-old and adult animals, the surface to volume ratio was greater in perihepatic cells than periportal cells, and the volume density was greater in periportal cells than perihepatic cells. The axial ratio became greater in perihepatic cells than periportal cells in adult animals. However, there were no differences in the surface density of the outer membrane, and of the inner membrane and cristae between the cells of both zones in all age groups examined. When the data were expressed as volume and area per cell, the patterns of subacinar distribution and age-related changes differed from the patterns seen in the volume and surface density data mainly in adult animals. This difference was generally caused by the marked increase in hepatocyte volume between 20 days of age and adulthood, especially in perihepatic cells. The results show that differences between mitochondria in periportal cells and those in perihepatic cells in the shape (the axial and surface to volume ratios), volume density, and area of the outer membrane per cell, evident in adult animals, are not present in newborn animals but arise during postnatal development.

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“…It is likely that this change is related to the sequence of the formation of adult lobulation that occurs during the postnatal period. LeBouton and Marchand (1970) and LeBouton (1974) suggested that postnatal change observed in the subacinar distribution of labeled nuclei after 3H-thymidine incorporation is caused by the drastic alteration at birth of the amount and composition of blood circulating through the liver. The fetal liver receives a relatively large volume of well-oxygenated blood, rich in nutrients, via the umbilical vein.…”

Section: Area Per Average Mononuclear Hepatocytementioning

confidence: 97%

“…Activities of glucose 6-phosphatase (Kanamura, 1975;Katz et al, 19761, ornithine carbamoyltransferase, succinate dehydrogenase, NADH dehydrogenase , bile canalicular alkaline phosphatase (DeWolf-Peeters et al, 1972), and labeled hepatocytes after 3H-thymidine injection (LeBouton and Marchand, 1970) are evenly distributed throughout the acinus within a few days after birth. The zonal distribution of these enzyme activities and of cells synthe-sizing DNA, characteristic of adult liver, appears gradually during postnatal development.…”

Section: Area Per Average Mononuclear Hepatocytementioning

confidence: 97%

Peri‐ and postnatal development of heterogeneity in the amounts of endoplasmic reticulum in mouse hepatocytes

1986

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The surface density and area per cell of the endoplasmic reticulum (ER) in periportal and perihepatic hepatocytes from male ddY mice, "17-, 18-, and 19-day-old fetuses," "newborn and 1-, 5-, 10-, and 20-day-old animals," and "adult animals" were analyzed by quantitative electron microscopy. The surface density of rough ER was not significantly different between periportal and perihepatic cells in all age groups examined, except for 19-day-old fetuses in which the value was greater in periportal cells than perihepatic cells. The surface density of smooth ER and total (rough and smooth) ER did not significantly differ between the periportal and perihepatic cells from 17-day-old fetuses to 5-day-old animals. In 10- and 20-day-old and adult animals, the values of smooth and total ER were greater in perihepatic cells than in periportal cells. When the data were expressed as area per cell, the patterns of subacinar distributions hardly differed, but age-related changes differed considerably from the patterns seen in the surface density data. The differences were generally caused by the increase in hepatocyte volume between 20 days of age and adulthood, especially in perihepatic cells, and by the changes in volume during the perinatal period. The results show that differences in the surface density and area per cell of smooth and total ER between periportal and perihepatic hepatocytes evident in adult animals are not present in fetal and newborn animals but arise during postnatal development.

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Changes in the distribution of thymidine-3H labeled cells in the growing liver acinus of neonatal rats

Cited by 36 publications

References 21 publications

Fine structure and function of hepatocytes during development

Fine structure and function of hepatocytes during development

Development of morphologic heterogeneity of hepatocyte mitochondria in the mouse

Peri‐ and postnatal development of heterogeneity in the amounts of endoplasmic reticulum in mouse hepatocytes

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