Changes in the expression of four ABC transporter genes in response to imidacloprid in Bemisia tabaci Q (Hemiptera: Aleyrodidae)

He, Chao; Liang, Jinjin; Liu, Shaonan; Wang, Shaoli; Wu, Qingjun; Xie, Wen; Zhang, Youjun

doi:10.1016/j.pestbp.2018.11.014

Cited by 57 publications

(32 citation statements)

References 58 publications

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“…Multiple ABC transporters belonging to different ABC subfamilies including ABCC, ABCG and ABCH, were upregulated in insecticide‐resistant strains of P. xylostella and Laodelphax striatellus . In addition, expression levels of ABC genes in different subfamilies were induced in Lepeophtheirus salmonis , Pediculus humanus humanus , Myzus persicae , Aedes aegypti , Nilaparvata lugens , Trichoplusia ni , B. tabaci Q and H. armigera after exposure to insecticides. The involvement of ABC transporters in insecticide resistance was further supported by reports that suppressed expression of ABC genes by RNA interference (RNAi) or genome editing technology could significantly increase insecticide toxicity .…”

Section: Discussionmentioning

confidence: 99%

Identification and transcriptional response of ATP‐binding cassette transporters to chlorantraniliprole in the rice striped stem borer, Chilo suppressalis

Meng

Yang

et al. 2020

Pest Management Science

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BACKGROUND: As the largest transporter gene family in metazoans, ATP-binding cassette (ABC) transporters regulate the efflux of a broad spectrum of substrates from the cytoplasm to the outside of the cell. In arthropods, ABC transporters are involved in phase III of the detoxification process, and play important roles in the metabolism and transport of insecticides. RESULTS: We identified 54 ABC transporters from the genome and transcriptome of Chilo suppressalis, one of the most damaging pests of rice in China. The identified ABC transporters were classified into eight subfamilies (ABCA to ABCH) based on NCBI BLAST and phylogenetic analysis. Synergism studies showed that treatment with verapamil, a potent inhibitor of ABC transporters, resulted in significantly increased toxicity of chlorantraniliprole against C. suppressalis larvae. Among the 21 tested ABC genes, three ABC transporter genes including CsABCC8, CsABCG1C and CsABCH1 were significantly upregulated after chlorantraniliprole treatment. CONCLUSION: ABC transporters play important roles in the detoxification and transport of chlorantraniliprole in C. suppressalis. The results from our study provide valuable information on C. suppressalis ABC transporters, and are helpful in understanding the roles of ABC transporters in chlorantraniliprole resistance mechanisms in C. suppressalis and other insect pests.

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Section: Discussionmentioning

confidence: 99%

Identification and transcriptional response of ATP‐binding cassette transporters to chlorantraniliprole in the rice striped stem borer, Chilo suppressalis

Meng

Yang

et al. 2020

Pest Management Science

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“…In the past few decades, B. tabaci has developed high levels of resistance to neonicotinoids in the field [27,33,34,35,36,37]. Previous studies have shown that the resistance of B. tabaci against neonicotinoid insecticides is mainly related to detoxification [7,8,34,38,40,57,58]. The enhanced activity of cytochrome P450 monooxygenases (P450s) is generally recognized as the major mechanism of resistance to imidacloprid in B. tabaci [34,38,39].…”

Section: Discussionmentioning

confidence: 99%

“…Silencing the GSTd7 gene increased the mortality of whiteflies exposed to imidacloprid [41], and knockdown of GST14 significantly increased the mortality of thiamethoxam-treated B. tabaci MED [40]. Recently, an ATP-binding cassette transporter, ABCG3, was shown to be involved in the resistance of B. tabaci to imidacloprid, and silencing the ABCG3 gene by RNAi increased the lethality of imidacloprid to adults of B. tabaci MED [58]. Previous transcriptome analysis revealed that the expression level of six COE genes of B. tabaci MED corresponded to the amount of imidacloprid to which individuals were exposed.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of Carboxylesterases (COEs) in the Whitefly, Bemisia tabaci (Gennadius)

Xia

Yang

et al. 2019

IJMS

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The whitefly (Bemisia tabaci), an important invasive pest that causes severe damage to crops worldwide, has developed resistance to a variety of insecticides. Carboxylesterases (COEs) are important multifunctional enzymes involved in the growth, development, and xenobiotic metabolism of insects. However, systematic studies on the COEs of B. tabaci are scarce. Here, 42 putative COEs in different functional categories were identified in the Mediterranean species of B. tabaci (B. tabaci MED) based on a genome database and neighbor-joining phylogeny. The expression patterns of the COEs were affected by the development of B. tabaci. The expression levels of six COEs were positively correlated with the concentration of imidacloprid to which B. tabaci adults were exposed. The mortality of B. tabaci MED adults fed dsBTbe5 (67.5%) and dsBTjhe2 (58.4%) was significantly higher than the adults fed dsEGFP (41.1%) when treated with imidacloprid. Our results provide a basis for functional research on COEs in B. tabaci and provide new insight into the imidacloprid resistance of B. tabaci.

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“…Real‐time quantitative PCR (qPCR) was carried out according to the MIQE (minimum information for publication of qPCR experiments) guidelines . The 20‐μL reaction system and program used were described in a previous paper . The length of the entire qPCR product was between 100 bp and 200 bp and was represented by a single band (Supporting Information Fig.…”

Section: Methodsmentioning

confidence: 99%

Genome‐wide identification and analysis of nuclear receptors genes for lethal screening against Bemisia tabaci Q

Liu

Liang

et al. 2020

Pest Management Science

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BACKGROUND: Nuclear receptors (NRs) play an essential role in diverse biological processes, such as insect metamorphosis. Here, transcriptome analysis and functional studies were used to determine whether NRs are involved in metamorphosis of whitefly Bemisia tabaci Q, a serious pest to crops, and to find some potential insecticide targets.RESULTS: Twenty NRs were identified in the Bemisia tabaci Q genome and categorized into the NR0-NR6 subfamilies. The phylogenetic tree of NRs from Bemisia tabaci Q and other representative species was constructed, which provided evolutionary insight into their genetic distances. The results of spatiotemporal gene expression indicated that the majority of NR gene expression was higher in the head than the abdomen and higher in eggs than adults. Further functional analysis using RNA interference (RNAi) showed that NR genes play an important role in Bemisia tabaci Q pupation and eclosion. With respect to high mortality and effects on growth, this was reflected in the unable to become pupa when the third-stage nymph treated with double-stranded RNA (dsRNA) and the developmental time delay (4-7 days) when pupae were treated with dsRNA for the 12 NR genes during molting compared with the development time in the control.CONCLUSION: This study provides insight into NR functions during the metamorphosis stages of Bemisia tabaci Q. Several candidate genes could be potential insecticide targets for whitefly pest control due to their important roles in insect development.

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Changes in the expression of four ABC transporter genes in response to imidacloprid in Bemisia tabaci Q (Hemiptera: Aleyrodidae)

Cited by 57 publications

References 58 publications

Identification and transcriptional response of ATP‐binding cassette transporters to chlorantraniliprole in the rice striped stem borer, Chilo suppressalis

Identification and transcriptional response of ATP‐binding cassette transporters to chlorantraniliprole in the rice striped stem borer, Chilo suppressalis

Genome-Wide Analysis of Carboxylesterases (COEs) in the Whitefly, Bemisia tabaci (Gennadius)

Genome‐wide identification and analysis of nuclear receptors genes for lethal screening against Bemisia tabaci Q

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