Changes in the female arcuate nucleus morphology and neurochemistry after chronic ethanol consumption and long-term withdrawal

Rebouças, E.C.C.; Leal, Sandra; Silva, Susana Maria; Sá, Susana I.

doi:10.1016/j.jchemneu.2016.05.001

Cited by 3 publications

(4 citation statements)

References 94 publications

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“…In a spot blot test, the MC-20 recognized its blocking peptide on nitrocellulose paper, and after adsorption of the primary antibody to its blocking peptide, no staining was observed (Fernández-Guasti et al 2000). In previous studies, we and other researchers have shown differences in distribution of the ERa using the same antibody in rat hypothalamus and preoptic area and one specific 68-kDa band in a western blot of rat hypothalamus (Kruijver et al 2002, Normandin & Murphy 2008, Garcia-Falgueras et al 2011, Malikov & Madeira 2012, Leite et al 2014, Martins et al 2015, Rebouças et al 2016.…”

Section: Antibody Characterizationmentioning

confidence: 69%

Dynamics of progesterone and estrogen receptor alpha in the ventromedial hypothalamus

Sá

Fonseca

2017

Journal of Endocrinology

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Cyclic fluctuations of estradiol and progesterone in females influence neuronal activity in the ventrolateral division of the ventromedial hypothalamic nucleus (VMNvl), through the activation of progesterone receptors (PRs) and estrogen receptors (ERs). The expression of ER and PR in the VMNvl is influenced by their cognate ligands and is a central upstream trigger in the pathway of VMNvl-dependent modulation of endocrine responses. By studying the role played by estradiol and progesterone in PR and ERa expression in the VMNvl along the estrous cycle and how the two receptors interact in the same neuron, we aim to evaluate the synergistic action of both ovarian hormones in the regulation of VMNvl activity. In animals at all phases of the estrous cycle, the number of VMN neurons expressing PR or ERa was estimated by stereological methods, and the percentage, and rostro-caudal distribution, of neurons simultaneously expressing both receptors was determined. The highest number of PR-immunoreactive neurons was seen at proestrus, and of ERa-immunoreactive neurons was seen at proestrus and metestrus. The ERa/PR co-localization is increased at caudal levels. Approximately half the neurons expressing PR co-express ERa, a proportion that stays constant along the estrous cycle. The percentage of ERa neurons co-expressing PR changes from 60% at proestrus to 40% at metestrus. Fluctuations in circulating ovarian hormone levels promote coordinated changes in PR and ERa expression and co-localization. This may be an important mechanism in the regulation of input relayed by the VMNvl, allowing a precise modulation of endocrine responses.

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Section: Antibody Characterizationmentioning

confidence: 69%

Dynamics of progesterone and estrogen receptor alpha in the ventromedial hypothalamus

Sá

Fonseca

2017

Journal of Endocrinology

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“…Ethanol increases the expression of ERR in the fly brain. Similar to the expression of ERR in fly heads, in mice, ESR1 is also expressed in the brain (40), and in rats, long-term exposure to ethanol increases ESR1 expression in the brain (41). ERR is a nuclear receptor that interacts with the transcription factor Sima/Hif-1α to regulate transcription (23).…”

Section: Discussionmentioning

confidence: 96%

ERR and dPECR Suggest a Link Between Neuroprotection and the Regulation of Ethanol Consumption Preference

Escarcena¹,

Neufeld²,

Rietschel³

et al. 2021

Front. Psychiatry

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Reconsumption of ethanol after withdrawal is a hallmark for relapse in recovering patients with alcohol use disorders. We show that the preference of Drosophila melanogaster to reconsume ethanol after abstinence shares mechanistic similarities to human behavior by feeding the antirelapse drug acamprosate to flies and reducing the ethanol consumption preference. The Drosophila cellular stress mutant hangover also reduced ethanol consumption preference. Together with the observation that an increasing number of candidate genes identified in a genome-wide association study on alcohol use disorders are involved in the regulation of cellular stress, the results suggest that cellular stress mechanisms might regulate the level of ethanol reconsumption after abstinence. To address this, we analyzed mutants of candidate genes involved in the regulation of cellular stress for their ethanol consumption level after abstinence and cellular stress response to free radicals. Since hangover encodes a nuclear RNA-binding protein that regulates transcript levels, we analyzed the interactions of candidate genes on transcript and protein level. The behavioral analysis of the mutants, the analysis of transcript levels, and protein interactions suggested that at least two mechanisms regulate ethanol consumption preference after abstinence—a nuclear estrogen-related receptor-hangover-dependent complex and peroxisomal trans-2-enoyl-CoA reductase (dPECR)-dependent component in peroxisomes. The loss of estrogen-like receptor and dPECR in neurons share a protective function against oxidative stress, suggesting that the neuroprotective function of genes might be a predictor for genes involved in the regulation of ethanol reconsumption after abstinence.

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“…Essa maior suscetibilidade feminina pode não se restringir ao consumo da droga. Estudo em animais demonstrou que após exposição crônica ao etanol, a abstinência não reverteu os danos causados e induziu neurodegeneração em áreas hipotalâmicas das fêmeas 9 . Os 70% que praticavam o binge drinking afirmaram ter iniciado o consumo de bebidas alcoólicas antes dos 16 anos de idade, o que está de acordo com trabalhos conduzidos em outros países que associaram o uso nocivo do etanol ao seu início precoce 7,19,27 .…”

Section: Discussionunclassified

“…Apesar disto, alguns trabalhos indicam que não há diferenças estatisticamente significantes no número de universitários de ambos os sexos no uso de bebidas alcoólicas 5,6,8 . Por sua vez, há evidências de maior suscetibilidade do sexo feminino aos transtornos causados por uso de bebida alcoolica 9,10 , inclusive socioculturais 11 .…”

Section: Introductionunclassified

O binge drinking por universitários: perfil do estudante e fatores de risco e proteção

Rebouças

Pereira

Lisbôa

et al. 2021

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Este é um estudo quantitativo realizado entre 2017 e 2018 em uma universidade pública estadual da Bahia, com o objetivo de avaliar a prática de binge drinking em universitários. Utilizou-se questionário autoaplicável e análise descritiva e inferencial. Participaram 150 universitários, sendo 52% mulheres e 48% homens que afirmaram ingerir bebidas alcoólicas, com média de idade de 23,2 anos (dp±3,6). Houve associação entre beber para amenizar a ansiedade e a prática do binge drinking (p=0,01); no uso moderado do álcool relatou-se não sentir nada ou sentir sonolência após o consumo da bebida, enquanto os que faziam uso nocivo, a sensação era de alegria (p<0,0001). No padrão de consumo típico do binge drinking, 41% eram mulheres e 59% eram homens. As diferenças estatisticamente significantes foram: quanto ao sexo masculino – ter sofrido ou causado prejuízos a terceiros (p=0,04); quanto ao sexo feminino – possuir benefício social (p=0,0001) e esconder dos pais que faziam uso excessivo do álcool (p=0,01). Os fatores de risco observados: sexo, início precoce do uso do álcool, ingresso na universidade e influência dos pares. Já os fatores de proteção: estar casado e ter religião. A universidade precisa entender seu papel nesse cenário incluindo atividades culturais e ofertando acesso a conhecimento sobre riscos e a redução do consumo de alcool nos universitários.

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Changes in the female arcuate nucleus morphology and neurochemistry after chronic ethanol consumption and long-term withdrawal

Cited by 3 publications

References 94 publications

Dynamics of progesterone and estrogen receptor alpha in the ventromedial hypothalamus

Dynamics of progesterone and estrogen receptor alpha in the ventromedial hypothalamus

ERR and dPECR Suggest a Link Between Neuroprotection and the Regulation of Ethanol Consumption Preference

O binge drinking por universitários: perfil do estudante e fatores de risco e proteção

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