Changes in the growth and biochemical composition of Chaetoceros calcitrans cultures using light-emitting diodes

Abstract

The marine microalgae Chaetoceros calcitrans was cultured under a fluorescent lamp (CON) and light-emitting diodes (LEDs) of various wavelengths (blue, LB; red, LR; green, LG; white, LW); changes in growth, fucoxanthin, chlorophyll-a, amino acid and fatty acid profiles were investigated. LR-exposed cultures exhibited the highest specific growth rate (SGR) (0.34), whereas LG-exposed cultures showed the lowest SGR (0.26). After cultivation for 10 days, the maximum dry cell weight (g/L) of LR-exposed cultures was significantly higher than that of those exposed to other light conditions (LR≧ CON>LB≧LW≧LG). Eicosapentaenoic acid (EPA) levels were significantly higher in CON-exposed cultures compared to those exposed to LW (P<0.05), with no marked difference compared to those exposed to LB, LR and LG (P>0.05). The fucoxanthin content was highest in LB-exposed cultures (6.3㎍/mL), whereas LW showed the lowest (3.6㎍/mL; P<0.05).Chlorophyll-a content was highest in cultures exposed to LB compared to other light sources. These results suggest consistent differences in growth and biochemical composition after exposure to light of different wavelengths.

Enhanced Production of Lutein by Indigenous Freshwater Microalga, Mychonastes pushpae Using Various Abiotic Stresses

“…그러나 같은 특정영양 염 결핍 조건에서도 미세조류 종에 따라 세포 성장 및 유용 산물의 생산에 미치는 영향이 다양하게 나타날 수 있다 [13,14]. 또한 해양 미세조류 배양에서와는 달리 담수 유래 미 세조류 배양에서 염도 (salinity)는 주된 스트레스 인자로 적 용될 수 있는 가능성이 확인되었으며 [15], 광합성을 하는 미 세조류의 경우 광도 및 광파장에 따라 미세조류 성장 및 유용 산물 생산에 영향을 미칠 수 있다 [16][17][18]…”