Changes in the human nuclear chromatin induced by ultra wideband pulse irradiation

Shckorbatov, Y. G.; Pasiuga, V. N.; Kolchigin, N. N.; Batrakov, Dmitry O.; Kazansky, O.V.; Калашников, В. В.

doi:10.2478/s11535-008-0051-4

Cited by 10 publications

(12 citation statements)

References 21 publications

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“…1/2. after electromagnetic cell exposure has been shown earlier (Shckorbatov et al, 2009a;Pasiuga et al, 2009), but in this paper for the first time we test the dependence of cell recovery on the intensity of microwave exposure. …”

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confidence: 69%

“…In our previous work we demonstrated condensation of chromatin in granules induced by an electromagnetic field (Shckorbatov et al, 1998;Shckorbatov et al, 2009a;2009b), but the process of recovery of microwave induced changes in chromatin microscopic morphology has remained almost uninvestigated. We observed an effect of time after microwave exposure and power density of irradiation on degree of chromatin condensation (Table 1).…”

Section: Discussionmentioning

confidence: 99%

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Cell nucleus and membrane recovery after exposure to microwaves

Shckorbatov¹,

Pasiuga²,

Kolchigin³

et al. 2011

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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Communicated by Aleksandrs RapoportsCells of human buccal epithelium of six male donors were exposed to microwave radiation (frequency f = 36.64 GHz, power density W = 0.1, 1 and 4 W/m 2 ). Exposure time was 10 seconds. The state of chromatin in cell nucleus was estimated by a number of heterochromatin granules after staining with 2% orcein in 45 % acetic acid. Permeability of cell membranes was estimated by percentage of unstained cells after 5 min of staining the cells with vital dyes trypan blue (0.5%) and indigocarmine (5 mM). Cell exposure to microwaves induced chromatin condensation (increase of the number of heterochromatin granules) and increase of membrane permeability to trypan blue and indigocarmine. Isolated human buccal cells demonstrated the ability to recover after microwave exposure. The number of heterochromatin granules decreased to its initial level after 0.5 hour (W = 0.1 W/m2 ) and 2 hours (W = 1 and 4 W/m 2 ) after cell exposure. Cell plasma membrane permeability recovered later -after 1 hour and 3 hours post exposure, respectively. Key words: human cell, chromatin, heterochromatin, buccal epithelium, electromagnetic radiation exposure. 13Proc. Latvian Acad. Sci., Section B, Vol. 65 (2011), No. 1/2. after electromagnetic cell exposure has been shown earlier (Shckorbatov et al., 2009a;Pasiuga et al., 2009), but in this paper for the first time we test the dependence of cell recovery on the intensity of microwave exposure. MATERIALS AND METHODSHuman cells. The studies were conducted using buccal epithelium human cells. The cells were obtained from the inner surface of donor's cheek by light scraping with a blunt sterile spatula. This operation was absolutely bloodless and painless. All the donors were informed about the purpose of investigation. Our investigations were performed in accordance with the European Convention on Human Rights and Biomedicine (1997), Declarations and Recommendations of the First, the Second and the Third National (Ukrainian) Congresses of Bioethics (Kiev, Ukraine, 2001, 2004, 2007 and Ukrainian legislation.The cells were placed in a 3.03 mM phosphate buffer (pH = 7.0) and 2.89 mM calcium chloride (Reachem, Moscow, Russia) solution, and stored for further experiments. 25 µl of cell suspension containing several thousand of cells were placed on a glass slide and subjected to microwave exposure. Cells were stained immediately after the exposure, 30 minutes, 1 hour, 2 and 3 hours after cell exposure with 2% orcein (Merck AG, Darmstadt, Germany) solution in 45 % acetic acid (Reachem, Moscow, Russia), 5 mM indigocarmine (Merck AG, Darmstadt, Germany) and 0.5% trypan blue (Reachem, Moscow, Russia) solutions. All cell donors were men, non-smokers. Ages of the donors A to F, respectively, were 18, 20, 24, 35, 53, and 55 years. The male donors were selected by the reasons of convenience, as in our previous experiments no gender-related differences between buccal epithelium cells in charateristics of chromatin condensation or membrane permeability was observed. For light mic...

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confidence: 69%

Section: Discussionmentioning

confidence: 99%

Cell nucleus and membrane recovery after exposure to microwaves

Shckorbatov¹,

Pasiuga²,

Kolchigin³

et al. 2011

Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.

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“…The simple method for determination of the portion of heterochromatin by counting the number of heterochromatin granules in a probe of cells and determination of average number per cell was proposed for buccal epithelium cells -cells of cheek mucosa [109]. In a series of works it was demonstrated that the low energy EMF (10 -100 µW/cm 2 ) and static magnetic field (25 mT) induce chromatin condensation (heterochromatinization) [38,47,[110][111][112][113][114]. It is supposed that this phenomenon reflects the process of regulation of genetic activity at cytological level, because heterochromatinization is connected with decrease of functional activity of chromatin [115,116].…”

Section: The Action Of Emf On Intracellular Regulatory Systemsmentioning

confidence: 99%

The Main Approaches of Studying the Mechanisms of Action of Artificial Electromagnetic Fields on Cell

Shckorbatov¹

2014

J Elec Electron Syst

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“…The intensity of UWBR was 10 -6 , 10 -5 , 10 -4 , 10 -3 , and 10 -2 W/cm 2 , exposure time -10 seconds. The experimental procedure is described in [5]. The short pulse generator formed the sequence of pulses with amplitude 25 V and a pulse duration 500 ps at the half of pulse height.…”

Section: Experimental Setup and Experimental Proceduresmentioning

confidence: 99%

Effects of ultra-wideband radiation on viability of human cells

Shckorbatov

Kolchigin

Kazansky

et al. 2012

2012 6th International Conference on Ultrawideband and Ultrashort Impulse Signals

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Effects of ultra-wideband radiation (UWBR) on isolated cells of human buccal epithelium are presented. Cells were isolated from human organism before experiment and suspension of cells was exposed during 10 seconds to UWBR of 10 -6 -10 -2 W/cm 2 intensity. In different periods after exposure to UWBR cells were stained with fluorescent stains Hoechst 3342 and ethidium bromide in purpose to assess their viability. The percentage of damaged cells was assessed by the standard criterion -the percentage of cells with nuclei stained red of pink by ethidium bromide. Cell exposure to UWBR of 10 -4 -10 -2 W/cm 2 results in increase of percentage of damaged cells. The percentage of damaged cells gradually decreases up to 3 hours after cell exposure. This phenomenon is interpreted as cell recovery after cell damage induced by UWBR. The data obtained are discussed in connection to the experimental results on UWBR upon cell nucleus structure obtained previously.

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Changes in the human nuclear chromatin induced by ultra wideband pulse irradiation

Cited by 10 publications

References 21 publications

Cell nucleus and membrane recovery after exposure to microwaves

Cell nucleus and membrane recovery after exposure to microwaves

The Main Approaches of Studying the Mechanisms of Action of Artificial Electromagnetic Fields on Cell

Effects of ultra-wideband radiation on viability of human cells

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