Changes in the Mechanical and Biochemical Properties of Aortic Tissue due to Cold Storage

Biomechanical Properties and Correlation With Collagen Solubility Profile in the Posterior Sclera of Canine Eyes With anADAMTS10Mutation

“…57 Previous studies also found only mild changes in collagen content due to short freezing times of soft tissues. 44,58 The hydration level of the sclera strips also was unknown, and could be greater than that of fresh, intact sclera due to the potential swelling during transport, mechanical testing, and freeze/thaw. This most likely decreased the magnitudes of lg collagen/mg wet weight for each strip, potentially making these data less comparable to the collagen content of fresh scleral tissue.…”

Human thoracic and abdominal aortic aneurysmal tissues: Damage experiments, statistical analysis and constitutive modeling

“…Venkatasubramanian et al (2006) investigated the changing mechanical behavior of femoral arteries from pigs due to freezing at À20 1C and À80 1C and concluded that major changes are only evident in the low-strain region of the mechanical response. In contrast, Chow and Zhang (2011) performed biaxial mechanical experiments on bovine thoracic aortas that had been stored at common storage temperatures (4 1C, À 20 1C, or À 80 1C) for three different time points (48 hours, 1 week, or 3 weeks). For a storage at À20 1C the authors found no significant change in the low-strain regime but a significant shift in the highstrain regime of the mechanical response.…”

“…For a storage at À20 1C the authors found no significant change in the low-strain regime but a significant shift in the highstrain regime of the mechanical response. Chow and Zhang (2011) concluded that there are changes within the tissue most likely due to intracellular ice formation. Additionally, O'Leary et al (2014b) tested the same porcine aortic tissue samples before and after freezing/storage at À 20 1C and found no significant difference in the mechanical properties determined by experiments.…”

Investigation of a small-diameter decellularised artery as a potential scaffold for vascular tissue engineering; biomechanical evaluation and preliminary cell seeding

“…Many different animals (Allaire et al, 1997;Chow and Zhang, 2011;Conklin et al, 2002;Dahan et al, 2012;Goissis et al, 2000;Liu et al, 2004;Schaner et al, 2004;Sheridan et al, 2012;Williams et al, 2009;Zhao et al, 2010) have been used to determine the viability of decellularisation methods on a variety of blood vessels including the aorta (Goissis et al, 2000;Liu et al, 2004), carotid artery (Conklin et al, 2002;Dahan et al, 2012;Sheridan et al, 2012;Williams et al, 2009;Zhao et al, 2010), iliac artery (Goissis et al, 2000), saphenous vein (Schaner et al, 2004) and iliac vein (Goissis et al, 2000) for the development of a TEBV. Combinations of chemical, enzymatic and physical treatments have been used (Gilbert et al, 2006) and the minimum decellularisation time reported has been 4 h for rat aorta where agitation in NaOH for 1 h followed by 3 h of vigorous agitation in PBS achieved rapid decellularisation (Liu et al, 2004).…”

“…Other studies have shown that the mechanical properties of biological materials alter dramatically with time once harvested from the body (Chow and Zhang, 2011;Stemper et al, 2007). Therefore the longer the decellularisation time the greater the natural degradation of the tissue which will have an effect on the mechanical properties of the resulting scaffold.…”