Changes in the mitochondrial calcium influx and efflux properties are responsible for the decline in sperm calcium during epididymal maturation

Vijayaraghavan, Srinivasan; Hoskins, Dale D.

doi:10.1002/mrd.1080250212

Cited by 36 publications

(35 citation statements)

References 27 publications

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“…The dramatic fall in extracellular Na C would induce the inhibition of both NHE and NCX, with subsequent accumulation of excess H C and Ca 2C in the sperm cytoplasm, followed by inhibition of the dynein-ATPase by means of the sensors located on ODAs and IDAs, and therefore inhibition of motility. ii) Cytoplasmic Ca 2C in sperm from the caput epididymidis is two to six times higher than that in sperm from the cauda region (Vijayaraghavan & Hoskins 1990). It has been suggested that this could be due to sperm acquisition of ATP2B4 from membranous vesicles in the epididymal luminal fluid during sperm transit throughout the epididymis (Patel et al 2013).…”

Section: Discussionmentioning

confidence: 98%

ATPases, ion exchangers and human sperm motility

Peralta-Arias¹,

Vívenes²,

Camejo³

et al. 2015

Reproduction

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Human sperm has several mechanisms to control its ionic milieu, such as the Na,K-ATPase (NKA), the Ca-ATPase of the plasma membrane (PMCA), the Na C /Ca 2C -exchanger (NCX) and the NaOn the other hand, the dynein-ATPase is the intracellular motor for sperm motility. In this work, we evaluated NKA, PMCA, NHE, NCX and dynein-ATPase activities in human sperm and investigated their correlation with sperm motility. Sperm motility was measured by Computer Assisted Semen Analysis. It was found that the NKA activity is inhibited by ouabain with two K i (7.9!10 K9 and 9.8!10 K5 M), which is consistent with the presence of two isoforms of a subunit of the NKA in the sperm plasma membranes (a1 and a4), being a4 more sensitive to ouabain. The decrease in NKA activity is associated with a reduction in sperm motility. In addition, sperm motility was evaluated in the presence of known inhibitors of NHE, PMCA and NCX, such as amiloride, eosin, and KB-R7943, respectively, as well as in the presence of nigericin after incubation with ouabain. Amiloride, eosin and KB-R7943 significantly reduced sperm motility. Nigericin reversed the effect of ouabain and amiloride on sperm motility. Dynein-ATPase activity was inhibited by acidic pH and micromolar concentrations of Ca 2C. We explain our results in terms of inhibition of the dynein-ATPase in the presence of higher cytosolic H C and Ca 2C, and therefore inhibition of sperm motility.

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Section: Discussionmentioning

confidence: 98%

ATPases, ion exchangers and human sperm motility

Peralta-Arias¹,

Vívenes²,

Camejo³

et al. 2015

Reproduction

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“…These data strongly suggest that CRISP4, via the regulation of Ca 2+ flow through TRPM8 channels, is involved in defining the functional competence of the sperm acrosome. Electrochemical gradients (K + , Na + , Ca 2+ , H + ) are established in sperm throughout epididymal maturation at or around the transition from caput to the corpus epididymis in the mouse (34)(35)(36). Changes in ion flow have been correlated with the acquisition of specific sperm functions normally manifest during capacitation (3).…”

Section: E)mentioning

confidence: 99%

Cysteine-rich secretory protein 4 is an inhibitor of transient receptor potential M8 with a role in establishing sperm function

Gibbs¹,

Orta

Reddy³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

100

110

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The cysteine-rich secretory proteins (CRISPs) are a group of four proteins in the mouse that are expressed abundantly in the male reproductive tract, and to a lesser extent in other tissues. Analysis of reptile CRISPs and mouse CRISP2 has shown that CRISPs can regulate cellular homeostasis via ion channels. With the exception of the ability of CRISP2 to regulate ryanodine receptors, the in vivo targets of mammalian CRISPs function are unknown. In this study, we have characterized the ion channel regulatory activity of epididymal CRISP4 using electrophysiology, cell assays, and mouse models. Through patch-clamping of testicular sperm, the CRISP4 CRISP domain was shown to inhibit the transient receptor potential (TRP) ion channel TRPM8. These data were confirmed using a stably transfected CHO cell line. TRPM8 is a major cold receptor in the body, but is found in other tissues, including the testis and on the tail and head of mouse and human sperm. Functional assays using sperm from wild-type mice showed that TRPM8 activation significantly reduced the number of sperm undergoing the progesteroneinduced acrosome reaction following capacitation, and that this response was reversed by the coaddition of CRISP4. In accordance, sperm from Crisp4 null mice had a compromised ability to undergo to the progesterone-induced acrosome reaction. Collectively, these data identify CRISP4 as an endogenous regulator of TRPM8 with a role in normal sperm function.cysteine-rich secretory proteins | antigen 5 | pathogenesis-related 1 | epididymal maturation | fertility

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“…As Ca 2+ readily crosses the plasma membrane (Vijayaraghavan and Hoskins, 1990) it would be necessary for the spermatozoa to either pump excess Ca 2+ out of the cell, or store this cation in subcellular locations such as the acrosome (Dorval et al, 2002). In either case, homeostatic regulation of Ca 2+ would be an energy-dependent process.…”

Section: Discussionmentioning

confidence: 99%

“…Changes in intracellular pH do not drive tyrosine phosphorylation It has been reported in bovine spermatozoa, that intracellular pH [pH]i is a regulator of intracellular calcium [Ca 2+ ]i concentrations (Vijayaraghavan and Hoskins, 1990 (Vijayaraghavan and Hoskins, 1990). Therefore, we examined whether the removal of Ca 2+ from the incubation medium resulted in rise in [pH]i in human spermatozoa.…”

Section: Calcium Negatively Regulates Tyrosine Phosphorylation In Hummentioning

confidence: 99%

“…4). Since it is established that Ca 2+ can diffuse through the sperm plasma membrane (Vijayaraghavan and Hoskins, 1990), [Ca 2+ ]i homeostasis must be maintained by pumping this cation into Ca 2+ stores within the spermatozoa, or across the plasma membrane, or both (for a review, see Breitbart, 2002). This being the case, the level of ATP in spermatozoa incubated in complete BWW, would expected to be lower than that incubated in BWW -Ca 2+ .…”

Section: Calcium Negatively Regulates Tyrosine Phosphorylation In Hummentioning

confidence: 99%

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64. Analysis of the mechanism by which calcium negatively regulates the tyrosine phosphorylation cascade associated with sperm capacitation

Baker¹,

Hetherington²,

Ecroyd³

et al. 2003

Reprod. Fertil. Dev.

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Changes in the mitochondrial calcium influx and efflux properties are responsible for the decline in sperm calcium during epididymal maturation

Cited by 36 publications

References 27 publications

ATPases, ion exchangers and human sperm motility

ATPases, ion exchangers and human sperm motility

Cysteine-rich secretory protein 4 is an inhibitor of transient receptor potential M8 with a role in establishing sperm function

64. Analysis of the mechanism by which calcium negatively regulates the tyrosine phosphorylation cascade associated with sperm capacitation

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