2010
DOI: 10.1534/genetics.110.119149
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Changes in the Nuclear Envelope Environment Affect Spindle Pole Body Duplication in Saccharomyces cerevisiae

Abstract: The Saccharomyces cerevisiae nuclear membrane is part of a complex nuclear envelope environment also containing chromatin, integral and peripheral membrane proteins, and large structures such as nuclear pore complexes (NPCs) and the spindle pole body. To study how properties of the nuclear membrane affect nuclear envelope processes, we altered the nuclear membrane by deleting the SPO7 gene. We found that spo7D cells were sickened by the mutation of genes coding for spindle pole body components and that spo7D w… Show more

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Cited by 40 publications
(64 citation statements)
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“…This includes the intriguing finding that the null allele of the essential MPS2 gene can be rescued by deletion of Pom152 or Pom34 (Sezen et al 2009). Similarly, the deletion of Pom152 or the nucleoporin Nup157 will rescue the deletion of the MPS3 gene, another essential SPB membrane protein (Witkin et al 2010). Finally, a screen for SPB-remodeling factors, described below, identified a role for Pom152 in controlling Figure 7 The SPB duplication pathway.…”
Section: Building the Spindlementioning
confidence: 97%
See 1 more Smart Citation
“…This includes the intriguing finding that the null allele of the essential MPS2 gene can be rescued by deletion of Pom152 or Pom34 (Sezen et al 2009). Similarly, the deletion of Pom152 or the nucleoporin Nup157 will rescue the deletion of the MPS3 gene, another essential SPB membrane protein (Witkin et al 2010). Finally, a screen for SPB-remodeling factors, described below, identified a role for Pom152 in controlling Figure 7 The SPB duplication pathway.…”
Section: Building the Spindlementioning
confidence: 97%
“…These interactions between SPBs and NPCs are complex and poorly understood, but involve shared components (Chial et al 1998), shared environment in the nuclear envelope (Witkin et al 2010), and coordinated translational control of component synthesis (Sezen et al 2009).…”
Section: Building the Spindlementioning
confidence: 99%
“…This phenotype can be rescued in a mutant of the NPC, Δpom152 ( Fig. S2; Witkin et al, 2010). Yet, Δpom152 itself has an effect on subtelomere organization (Fig.…”
Section: Antagonistic Effects Of Csm4 and Polymerized Actin On Subtelmentioning
confidence: 98%
“…The SPB is then inserted into a pore in the NE, allowing for assembly of nuclear components to create duplicated side-by-side SPBs (Byers and Goetsch 1974;Byers and Goetsch 1975;Adams and Kilmartin 1999;Jaspersen and Winey 2004;Winey and Bloom 2012). The membrane anchors and half-bridge components both play a role in this SPB insertion step (Winey et al 1991(Winey et al , 1993Schramm et al 2000;Araki et al 2006;Sezen et al 2009;Witkin et al 2010;Friederichs et al 2011;Kupke et al 2011;Winey and Bloom 2012). Unlike NPC assembly, SPB duplication is spatially and temporally restricted.…”
mentioning
confidence: 99%
“…One of the SPB membrane anchors is Ndc1, a conserved integral membrane protein that is also an essential NPC Pom and required for NPC assembly Mansfeld et al 2006;Stavru et al 2006;Kind et al 2009). Some NPC components are required for proper remodeling of SPB core components and regulation of SPB size (Niepel et al 2005;Greenland et al 2010), whereas the loss of other NPC components rescues SPB mutant assembly phenotypes Sezen et al 2009;Witkin et al 2010). The exact mechanism by which all of these NPC components influence SPB assembly is not known.…”
mentioning
confidence: 99%