Changes in the organization of the sea urchin egg plasma membrane upon fertilization: Indications from the lateral diffusion rates of lipid-soluble fluorescent dyes

Wolf, David E.; Kinsey, William H.; Lennarz, William J.; Edidin, Michael

doi:10.1016/0012-1606(81)90355-9

Cited by 118 publications

(57 citation statements)

References 12 publications

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“…Partial mobility of the lipid probe might be due to the existence of lipid microdomains but at present no evidence for this is available in the Xenopus egg. The occurrence of lipid microdomains as detected by partial recovery of a lipid probe, seems to be a characteristic of the plasma membrane in early embryonic cells and has been reported earlier for sea urchin eggs (Campisi and Scandella, 1980;Wolf et a& 1981a) and mouse eggs (Wolf et a& 1981b;Klausner and Wolf, 1980;Peter and Richter, 1981). From another study (Gadenne et al, 1984) we know that in dissociated early blastula cells the MF value of the surface membrane is still 72.5 f 4.6% but the D value is lower: 2.39 X lo-' cm'/sec.…”

Section: Discussionsupporting

confidence: 67%

Lateral mobility of plasma membrane lipids in dividing Xenopus eggs

Tetteroo

Bluemink

Dictus

et al. 1984

Developmental Biology

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Section: Discussionsupporting

confidence: 67%

Lateral mobility of plasma membrane lipids in dividing Xenopus eggs

Tetteroo

Bluemink

Dictus

et al. 1984

Developmental Biology

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“…linkage of membrane lipids and proteins with elements of cytoskeleton and cytomusculature [2], can induce a macroheterogeneity in the membrane. Moreover, theoretical models and experimental evidence suggest the possibility of microheterogeneity in the structural organization of model and natural membranes [3][4][5][6][7][8]. In a bilayer containing a single phospholipid, structural defects have been demonstrated by a variety of techniques, and these defects have been ascribed to conformational disorder of acyl chains related to trans-gauche isomerization [3].…”

Section: Introductionmentioning

confidence: 99%

Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene reveal the effect of cholesterol on the microheterogeneity of erythrocyte membrane

Fiorini

Valentino

Gläser

et al. 1988

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene reveal the effect of cholesterol on the microheterogeneity of erythrocyte membrane The fluorescence decay of 1,6 diphenyl-l,3,5-hexatriene (DPH) has been used to characterize aspects of the erythrocyte membrane structure related to the microheterogeneity of the lipid bilayer. The DPH decay has been studied using frequency domain fluorometry and the data analyzed either by a model of discrete exponential components or a model that assumes a continuous distribution of lifetime values. The main intensity fraction was associated with a lifetime value centered at about 11 ns in the erythrocyte membrane, but a short component of very low fractional intensity had to be considered to obtain a good fit to the data. The lifetime value of the long component was insensitive to temperature, while the width of the distribution decreased with increasing temperature. In multilamellar liposomes prepared from phospholipids extracted from the erythrocytes, the long lifetime component showed a temperature dependence. The depletion of 27% of the cholesterol in the erythocyte membrane induced a broadening of the distribution, suggesting a homogenizing effect of cholesterol. This effect has also been detected in egg phosphatidylcholine at a very low cholesterol/phospholipid molar ratio. The role of cholesterol on membrane heterogeneity is discussed in relation to the effect of cholesterol on water penetration.

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“…Such domains could be significant as regulators of the enzymatic activity of integral and peripheral membrane proteins and may also serve as topological organizers for cell membrane receptors. Although small (a few lipid-molecule diameters) (4) and large (.300 nm in diameter) (4,5) lipid domains have been observed in model liposome systems, only small lipid patches of a particular phase have been shown to exist in natural biological systems (6,7). Using the technique of fluorescence redistribution after photobleaching (FRAP), a number ofinvestigators have shown that in model binary phospholipid systems, distinct large lipid domains can be detected by the variation in diffusion components and bleaching recoveries of a fluorescent probe that can be partitioned between the fluid and gel phases (8,9).…”

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confidence: 99%

Lateral diffusion of phospholipids in the plasma membrane of soybean protoplasts: Evidence for membrane lipid domains

Metcalf

Wang²,

Schindler³

1986

Proc. Natl. Acad. Sci. U.S.A.

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Fluorescent lipid and phospholipid probes were incorporated at 40C into soybean protoplasts prepared from cultured soybean (SB-1) cells. Fluorescence microscopy showed that the plasma membrane as well as the nucleus were labeled. Fluorescence redistribution after photobleaching (FRAP) analysis was performed on these cells at 18°C to monitor the lateral mobility of the incorporated probes. After labeling at low concentrations (40 pg/ml) of phosphatidyl-N-(4-nitrobenzo-2-oxa-1,3-diazolyl)ethanolamine (NBD-PtdEtn), a single mobile component was observed with a diffusion coefficient (D) of w3 X 10-9 cm2/sec. After labeling at higher probe concentrations ('100 jig/ml), two diffusing species were observed, with diffusion coefficients of "'3 x 10-cm2/sec ("fast") and m5 x 10-10 cm2/sec ("slow"). Similar results were observed with fluorescent derivatives of phosphatidylcholine and fatty acids. In contrast to these results, parallel analysis of 3T3 fibroblasts, using the same probes and conditions, yielded only a single diffusion component. These results suggest that the soybean plasma membrane may contain two distinct lipid domains in terms oflipid mobility. Consistent with this idea, experiments with soybean protoplasts yielded a single diffusion component under the following conditions: (i) labeling with NBD-PtdEtn (100 ,ug/ml), FRAP analysis at 37C (D = 1.1 x 10-8 cm2/sec); (ii) labeling with NBD-PtdEtn (100 pg/ml), FRAP analysis at 180C in the presence of 2 mM EGTA (D = 4.2 x 10' cm2/sec); (iii) labeling with 5-(Ndodecanoyl)aminofluorescein (a short-chain lipid probe), FRAP analysis at 18'C or 3rC (D = 2.5 x 10-8 cm2/sec). (8,9). These measurements, however, failed to demonstrate any major differences in the diffusion coefficient (D) of a lipid probe in mammalian plasma membranes (10).We have reported diffusion coefficients for lectin-receptor complexes on the plasma membrane of soybean protoplasts derived from the SB-1 cell line (11). Here we extend the analysis of the lateral mobility of the components of the soybean cell plasma membrane by studying the diffusion of fluorescent lipid analogs. The data suggest that the soybean membrane may be a composite of large-scale, immiscible gel and fluid lipid domains. This suggestion is particularly intriguing in light of the higher content of negatively charged phospholipid species, particularly phosphatidylglycerol, in plant plasma membranes (12), as compared to mammalian plasma membranes (13). In this context, Verkleij et al. (14) and Ohnishi and Ito (15) have reported that phase separation and the formation of negatively charged phospholipid domains occurs after addition of Ca2+ to mixed phospholipid model systems. MATERIALS AND METHODSMaterials. The fluorescent probes used in this study were 1-acyl-2-[N-(4-nitrobenzo-2-oxa-1,3-diazoyl)amino]caproylsn-glycero-3-phosphocholine (NBD-PtdCho), phosphatidyl-N-(4-nitrobenzo-2-oxa-1,3-diazolyl)ethanolamine (NBDPtdEtn), 1,1'-dioctadecyl-3,3,3' ,3'-tetramethylindocarbocyanine iodide (diC18Icc), 1,1'-ditetradecyl-3,3-3',...

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Changes in the organization of the sea urchin egg plasma membrane upon fertilization: Indications from the lateral diffusion rates of lipid-soluble fluorescent dyes

Cited by 118 publications

References 12 publications

Lateral mobility of plasma membrane lipids in dividing Xenopus eggs

Lateral mobility of plasma membrane lipids in dividing Xenopus eggs

Fluorescence lifetime distributions of 1,6-diphenyl-1,3,5-hexatriene reveal the effect of cholesterol on the microheterogeneity of erythrocyte membrane

Lateral diffusion of phospholipids in the plasma membrane of soybean protoplasts: Evidence for membrane lipid domains

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