Changes in the Population of Methicillin-Resistant Staphylococcus pseudintermedius and Dissemination of Antimicrobial-Resistant Phenotypes in the Netherlands

Duim, Birgitta; Verstappen, Koen M.; Broens, Els M.; Laarhoven, Laura M.; Duijkeren, Engeline van; Hordijk, Joost; Heus, Phebe de; Spaninks, Mirlin; Timmerman, Arjen J.; Wagenaar, Jaap A.

doi:10.1128/jcm.01288-15

Cited by 58 publications

(77 citation statements)

References 30 publications

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“…All identified STs and their clonal and genetic relatedness are presented in Figure 3(a and b). Six STs grouped together with previously described CCs (CC45 and CC258) (Figure 3a) 14 . STs in the CC258 group were scattered in the sequence comparison tree, while STs in the CC45 group were more alike (Figure 3b).…”

Section: Resultsmentioning

confidence: 58%

“…Additionally, CC258 has recently been reported as a major CC in both the Netherlands 14 and Denmark 45 . These studies also reported a marked diversity in the MRSP population in recent years 14 , 45 . It appears that ST71 has lost its sustainability and CC258 is only filling the gap as a more successful lineage.…”

Section: Discussionmentioning

confidence: 87%

“…Representatives of this CC have been detected at least in Sweden, 47 the Netherlands 14 and in Israel and Thailand, 48 , 49 but have been rather rare in reports from Europe 14 , 47 . Representatives of ST45 are typically non-typeable by SmaI PFGE, 48 as was the case in our study.…”

Section: Discussionmentioning

confidence: 96%

“…Analysis was conducted at double- and triple-locus variant levels. Single- and double-locus variants of previously described clonal complexes (CCs) were assigned to that CC 14 . The number of isolates per ST or CC per year was calculated based on the specimen collection date.…”

Section: Methodsmentioning

confidence: 99%

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Antimicrobial resistance inStaphylococcus pseudintermediusand the molecular epidemiology of methicillin-resistantS. pseudintermediusin small animals in Finland

Grönthal

Eklund

Thomson

et al. 2017

J. Antimicrob. Chemother.

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Objectives: To investigate antimicrobial susceptibility in Staphylococcus pseudintermedius and the occurrence of methicillin-resistant S. pseudintermedius (MRSP), to explore the molecular structure of the MRSP population and to analyse risk factors for MRSP.Methods: Susceptibility data for clinical S. pseudintermedius isolates in 2011–15 were analysed using WHONET. All MRSP isolates in 2010–14 (n = 362) were typed using PFGE. Representative isolates (n = 87) of clusters were analysed using MLST and staphylococcal cassette chromosome mec (SCCmec) typing. Risk factors were analysed using logistic regression.Results: Of the clinical S. pseudintermedius (n = 1958; 98% from dogs), 14% were MRSP. Resistance to other antimicrobials varied between 12% and 39%. No trends were observed over time. Among clinical specimens (from infection sites) and screening specimens (from potential carriers), respectively, 2.5% (267/10 813) and 9% (211/2434) revealed MRSP. MLST revealed 42 different STs, including 19 new ones. Clonal complexes 71, 45 and 258 were the most common, but the MRSP population diversified over the years. A clinical S. pseudintermedius isolate was more likely to be MRSP if the patient was on antimicrobials at the time of sampling or was male. The presence of MRSP in screening specimens was more likely if the patient was on multiple antimicrobials at the time of sampling. Specimens from private clinics (versus the Veterinary Teaching Hospital of the University of Helsinki) had a higher likelihood of MRSP in both analyses.Conclusions: Resistance to antimicrobials among S. pseudintermedius in Finland is high, emphasizing the importance of infection control measures and susceptibility testing prior to therapy. The diverse MRSP population indicates non-clonal spread.

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Section: Resultsmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

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Antimicrobial resistance inStaphylococcus pseudintermediusand the molecular epidemiology of methicillin-resistantS. pseudintermediusin small animals in Finland

Grönthal

Eklund

Thomson

et al. 2017

J. Antimicrob. Chemother.

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“…These results correlated with other studies. 3,4 Risk factors for MRSP have been reported to be chronic skin infections, multiple hospital visits and the use of broad-spectrum antibiotics.…”

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confidence: 99%

Resisting the resistance. Is there progress in maintaining antimicrobial efficacy?

Lloyd

Varjonen

2017

Advances in Veterinary Dermatology

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(UK) discussed the major health threat caused by multiple drug resistance affecting both human and animal welfare, and the anticipation there will be limited future availability of new antibiotics, particularly in veterinary medicine. She briefly outlined antimicrobial treatment history, from the recognition of the importance of hand hygiene, to the recent golden era of antibiotic availability and efficacy, to the current 'period of disenchantment'; a time when antibiotics are starting to be of limited benefit due to increasing development of resistance.1 She then discussed the main pathogens of concern in human medicine, including Gram-negative organisms, and compared that to current veterinary concerns. In her practice, Vanessa Schmidt sees methicillinresistant Staphylococcus pseudintermedius (MRSP) as the greatest concern, but also methicillin-resistant Staphylococcus aureus and Staphylococcus schleiferi (MRSA and MRSS). Multidrug resistance (MDR) is also emerging in Gram-negative bacteria such as Escherichia coli, Pseudomonas, Proteus, Klebsiella and Acinetobacter spp. from cases of deep pyoderma. Multidrug-resistant enterococci are also becoming a concern.Vanessa Schmidt emphasized that it is important for clinicians to have knowledge of the local and regional antimicrobial resistance situation to help them in empirical clinical decision-making and also to be aware of when an empirical choice would be less appropriate. She presented current data and regional patterns for antimicrobial resistance in the UK. Nationwide data can be accessed online on Pet Resist.a This website provides antimicrobial resistance data from laboratory submissions to IDEXX UK, shown plotted on a map based on where submissions originate. The data lack information indicating from which body regions samples were taken, and on the numbers of isolates the percentages are based upon, but still provide updated information on regional resistance patterns. Data from a study conducted in Liverpool were presented. 2This laboratory-based study analysed S. pseudintermedius data from submissions to the university veterinary laboratory in Liverpool. Bacterial culture results were examined over time, and the site of infection was recorded. The data showed a significant increase in resistance over the past 5 years to clindamycin, gentamicin and fluoroquinolones, as well as MDR. Soft tissueand skin-derived isolates were more resistant than isolates from urine. These results correlated with other studies.3,4 Risk factors for MRSP have been reported to be chronic skin infections, multiple hospital visits and the use of broad-spectrum antibiotics.Oxacillin (methicillin) resistance between 2004 and 2015 was also reviewed in Liverpool. Cases of MRSP, which were first seen in 2007, have since increased year by year. This correlates with international data. The methicillin-sensitive S. pseudintermedius (MSSP) strains showed a high level of clindamycin resistance. The level of resistance to clindamycin in this study was higher when compared to levels reporte...

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Comparison of the prevalence, antibiotic resistance patterns, and biofilm formation ability of methicillin-resistant Staphylococcus pseudintermedius in healthy dogs and dogs with skin infections

Naziri

Majlesi

2022

Vet Res Commun

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Changes in the Population of Methicillin-Resistant Staphylococcus pseudintermedius and Dissemination of Antimicrobial-Resistant Phenotypes in the Netherlands

Cited by 58 publications

References 30 publications

Antimicrobial resistance inStaphylococcus pseudintermediusand the molecular epidemiology of methicillin-resistantS. pseudintermediusin small animals in Finland

Antimicrobial resistance inStaphylococcus pseudintermediusand the molecular epidemiology of methicillin-resistantS. pseudintermediusin small animals in Finland

Resisting the resistance. Is there progress in maintaining antimicrobial efficacy?

Comparison of the prevalence, antibiotic resistance patterns, and biofilm formation ability of methicillin-resistant Staphylococcus pseudintermedius in healthy dogs and dogs with skin infections

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