Changes in the prevalence and biofilm formation of Haemophilus influenzae and Haemophilus parainfluenzae from the respiratory microbiota of patients with sarcoidosis

Kosikowska, Urszula; Rybojad, Paweł; Stępień–Pyśniak, Dagmara; Żbikowska, Anna; Malm, Anna

doi:10.1186/s12879-016-1793-7

Cited by 21 publications

(17 citation statements)

References 49 publications

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“…More detailed studies on the ability to form biofilm by haemophili rods isolated from patients with OME will continue in the future. The high ability to grow in biofilm form especially of H. parainfluenzae and at low degree of H. influenzae was shown during our studies in pediatric patients with recurrent respiratory infections undergoing adenoidectomy [40], as well as in adult patients with sarcoidosis [41]. Bacteria in biofilms are generally well protected against environmental compounds, antimicrobials and the host immune system, and this is the reason why they are extremely difficult to eradicate [42][43][44][45].…”

Section: Discussionsupporting

confidence: 53%

Haemophilus influenzae and Haemophilus parainfluenzae occurrence in the ear effusion in pediatric patients prone to recurrent respiratory tract infections (RRTI) and with otitis media with effusion (OME)

Kosikowska

Chwiejczak

Niedzielski

et al. 2019

Current Issues in Pharmacy and Medical Sciences

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short duration of illness and an acute beginning. OME is the clinical term for the presence of fluid in the middle ear behind the intact eardrum and is characterized by a nonpurulent effusion that may be either mucoid (thick) or serous (thin) [4]. It is an inflammation of the middle ear without signs or symptoms of acute ear infection, pending beyond its intact eardrum and without systemic or otoscopic symptoms. An OME is the catchall term if the tympanic effusion persists for more than 3 months. Near chronic ARTICLE INFO ABSTRACT Introduction. Haemophilus influenzae and Haemophilus parainfluenzae are known as human-restricted respiratory microbiota representatives. The aim of the present paper was to assay haemophili prevalence in middle ear effusion specimens in pediatric patients with otitis media with effusion (OME). Methods. A total of 86 ear effusion specimens (from the left and right ear independently) were collected from 43 pediatric patients with OME. For comparison, 58 nasopharyngeal specimens were taken from 58 pediatric patients prone to recurrent respiratory tract infections (RRTI). Isolation and identification of haemophili biotypes and antimicrobial susceptibility was accomplished by standard microbiological methods. The cell surface hydrophobicity (CSH) of isolates was assayed by the method of aggregation in ammonium sulfate (SAT). Results. Haemophili were isolated in 25.6% (11/43) of all OME patients: in 5/43 (11.6%) -H. influenzae (biotypes II, III), in 5/43 (11.6%) -H. parainfluenzae, in 1/43 (2.3%) -both species were found. Haemophili-positive nasopharyngeal specimen was found in 27/58 (46.6%) RRTI patients: in 19/58 (32.8%) -H. influenzae, in 8/58 (13.8%) -H. parainfluenzae. About 90% of all haemophili isolates were characterised by extreme to strong CSH. Antimicrobial resistance occurred mainly among H. parainfluenzae (80%) and to a much lower percentage among H. influenzae (33.3%) isolates. The obtained data suggest that both H. influenzae and H. parainfluenzae can be involved in pathology of OME in pediatric patients. The high cell surface hydrophobicity can affect on the haemophili prevalence and ear colonization, and induces predisposition to the presence of these bacteria as a biofilm that serves as a virulence factor with great importance for the survival of these opportunistic bacteria and their persistence in the ear environment.

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Section: Discussionsupporting

confidence: 53%

Haemophilus influenzae and Haemophilus parainfluenzae occurrence in the ear effusion in pediatric patients prone to recurrent respiratory tract infections (RRTI) and with otitis media with effusion (OME)

Kosikowska

Chwiejczak

Niedzielski

et al. 2019

Current Issues in Pharmacy and Medical Sciences

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“…Apparently, it also occurs on spectacles in rather high shares. Figure 3 indicates that bacterial genera from the human aerodigestive tract were also frequently detected on the investigated spectacles, such as Moraxella, Haemophilus, Actinomyces, Capnocytophaga or Lautropia [24][25][26][27] . Coughing, sneezing or cleaning with clothes after breathing on the lenses might promote this diversity.…”

Section: Discussionmentioning

confidence: 99%

Site-specific molecular analysis of the bacteriota on worn spectacles

Fritz

März

Weis

et al. 2020

Sci Rep

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Regularly touched surfaces are usually contaminated with microorganisms and might be considered as fomites. The same applies for spectacles, but only little is known about their microbial colonization. Previous cultivation-based analyses from our group revealed a bacterial load strongly dominated by staphylococci. To better account for aerotolerant anaerobes, slow growing and yet-uncultivated bacteria, we performed an optimized 16S rRNA gene sequencing approach targeting the V1-V3 region. 30 spectacles were swab-sampled at three sites, each (nosepads, glasses and earclips). We detected 5232 OTUs affiliated with 19 bacterial phyla and 665 genera. Actinobacteria (64%), Proteobacteria (22%), Firmicutes (7%) and Bacteroidetes (5%) were relatively most abundant. At genus level, 13 genera accounted for 84% of the total sequences of all spectacles, having a prevalence of more than 1% relative abundance. Propionibacterium (57%), Corynebacterium (5%), Staphylococcus (4%), Pseudomonas, Sphingomonas and Lawsonella (3%, each) were the dominant genera. Interestingly, bacterial diversity on the glasses was significantly higher compared to nosepads and earclips. Our study represents the first cultivation-independent study of the bacteriota of worn spectacles. Dominated by bacteria of mostly human skin and epithelia origin and clearly including potential pathogens, spectacles may play a role as fomites, especially in clinical environments.

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“…Biofilms are generally defined as communities of bacteria attached to a substratum or interface that are encased in an extracellular polymeric matrix material and exhibit resistance to exogenous stressors (4,43,44). Recently, H. parainfluenzae biofilm formation was observed in vitro using a microtiter plate assay with crystal violet staining (33,34); however, the structure and components of H. parainfluenzae biofilms remain unknown. The results in the present study clearly demonstrated that H. parainfluenzae formed smooth biofilms containing dense and multiple layers of viable and dead bacteria without pillar and tower structures on the surface.…”

Section: Discussionmentioning

confidence: 99%

“…These diseases are often chronic/recurrent in nature and may involve bacterial persistence within biofilm communities, which are beneficial for bacterial persistence and antibiotic resistance. Recently, studies using static biofilm assays showed that H. parainfluenzae can form biofilms in vitro (33,34); however, the structure and matrix components of the biofilms are unknown and there is no current study to test the impact of biofilm formation/ maturation by H. parainfluenzae in vivo.…”

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confidence: 99%

Haemophilus parainfluenzae Strain ATCC 33392 Forms BiofilmsIn Vitroand during Experimental Otitis Media Infections

Pang

Swords

2017

Infect Immun

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is a nutritionally fastidious, Gram-negative bacterium with an oropharyngeal/nasopharyngeal carriage niche that is associated with a range of opportunistic infections, including infectious endocarditis and otitis media (OM). These infections are often chronic/recurrent in nature and typically involve bacterial persistence within biofilm communities that are highly resistant to host clearance. This study addresses the primary hypothesis that forms biofilm communities that are important determinants of persistence The results from biofilm studies confirmed that formed biofilm communities within which the polymeric matrix was mainly composed of extracellular DNA and proteins. Using a chinchilla OM infection model, we demonstrated that formed surface-associated biofilm communities containing bacterial and host components that included neutrophil extracellular trap (NET) structures and that the bacteria mainly persisted in these biofilm communities. We also used this model to examine the possible interaction between and its close relative , which is also commonly carried within the same host environments and can cause OM. The results showed that coinfection with promoted clearance of from biofilm communities during OM infection. The underlying mechanisms for bacterial persistence and biofilm formation by and knowledge about the survival defects of during coinfection with are topics for future work.

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Changes in the prevalence and biofilm formation of Haemophilus influenzae and Haemophilus parainfluenzae from the respiratory microbiota of patients with sarcoidosis

Cited by 21 publications

References 49 publications

Haemophilus influenzae and Haemophilus parainfluenzae occurrence in the ear effusion in pediatric patients prone to recurrent respiratory tract infections (RRTI) and with otitis media with effusion (OME)

Haemophilus influenzae and Haemophilus parainfluenzae occurrence in the ear effusion in pediatric patients prone to recurrent respiratory tract infections (RRTI) and with otitis media with effusion (OME)

Site-specific molecular analysis of the bacteriota on worn spectacles

Haemophilus parainfluenzae Strain ATCC 33392 Forms BiofilmsIn Vitroand during Experimental Otitis Media Infections

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