Changes in the Proanthocyanidin Composition and Related Gene Expression in Bilberry (<i>Vaccinium myrtillus</i>L.) Tissues

Suvanto, Jussi; Karppinen, Katja; Riihinen, Kaisu R.; Jaakola, Laura; Salminen, Juha‐Pekka

doi:10.1021/acs.jafc.0c02158

Cited by 19 publications

(14 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8b) accompanied by the significant downregulation of VmCHS , VmF3′5′H , VmANS and VmLAR1a expression. This suggests that VmMYBPA1.1 is an important regulator of berry anthocyanin biosynthesis and delphinidin branch genes (Suvanto et al ., 2020). We also found that VmUFGT expression was downregulated, but not significantly, by contrast with significant upregulation of VmLDOX , and slight but not significant upregulation of VmLAR2 and VmANR (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…To investigate the spatial and temporal expression patterns of the VmMYB genes and to correlate the expression to their target genes for providing clues into their function, the measurements of transcript abundance followed by hierarchical clustering analysis were performed for various tissues (berry, leaf, stem, rhizome) as well as for different stages of berry development. In bilberry, PAs accumulate at the early stages of berry development while anthocyanins begin to accumulate at fruit ripening (Jaakola et al ., 2002; Karppinen et al ., 2016; Suvanto et al ., 2020). Our results revealed that the transcripts of VmMYBA1 and VmMYBPA1.1 were most highly associated with ripening fruit, showing a similar expression pattern to the structural genes related to anthocyanin biosynthesis (Figs 3, S2, S3), suggesting the involvement in regulation of anthocyanin accumulation in berry.…”

Section: Resultsmentioning

confidence: 99%

“…To deepen our understanding of the regulatory role of R2R3 MYBs in flavonoid biosynthesis in blue‐coloured berries, we focused on wild European bilberry ( Vaccinium myrtillus ), which has an active flavonoid and anthocyanin metabolism. Anthocyanins accumulate in both peel and flesh at berry ripening stage, while vegetative parts are rich with PAs, which also accumulate in the berry at early developmental stages (Jaakola et al ., 2002; Karppinen et al ., 2016; Suvanto et al ., 2020). Due to the similar complex anthocyanin profile in both peel and flesh, with a total of 33 different anthocyanin compounds belonging to the delphinidin, cyanidin, petunidin, peonidin and malvidin classes (Jaakola et al ., 2002; Riihinen et al ., 2008; Zoratti et al ., 2014), bilberry has in recent years become an attractive fruit species for studying regulation of anthocyanin biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

MYBA and MYBPA transcription factors co‐regulate anthocyanin biosynthesis in blue‐coloured berries

et al. 2021

Self Cite

View full text Add to dashboard Cite

Summary The regulatory network of R2R3 MYB transcription factors in anthocyanin biosynthesis is not fully understood in blue‐coloured berries containing delphinidin compounds. We used blue berries of bilberry (Vaccinium myrtillus) to comprehensively characterise flavonoid‐regulating R2R3 MYBs, which revealed a new type of co‐regulation in anthocyanin biosynthesis between members of MYBA‐, MYBPA1‐ and MYBPA2‐subgroups. VmMYBA1, VmMYBPA1.1 and VmMYBPA2.2 expression was elevated at berry ripening and by abscisic acid treatment. Additionally, VmMYBA1 and VmMYBPA1.1 expression was strongly downregulated in a white berry mutant. Complementation and transient overexpression assays confirmed VmMYBA1 and VmMYBA2 to induce anthocyanin accumulation. Promoter activation assays showed that VmMYBA1, VmMYBPA1.1 and VmMYBPA2.2 had similar activity towards dihydroflavonol 4‐reductase (DFR) and anthocyanidin synthase (ANS), but differential regulation activity for UDP‐glucose flavonoid 3‐O‐glucosyltransferase (UFGT) and flavonoid 3′5′‐hydroxylase (F3′5′H) promoters. Silencing of VmMYBPA1.1 in berries led to the downregulation of key anthocyanin and delphinidin biosynthesis genes. Functional analyses of other MYBPA regulators, and a member of novel MYBPA3 subgroup, associated them with proanthocyanidin biosynthesis and F3′5′H expression. The existence of 18 flavonoid‐regulating MYBs indicated gene duplication, which may have enabled functional diversification among MYBA, MYBPA1 and MYBPA2 subgroups. Our results provide new insights into the intricate regulation of the complex anthocyanin profile found in blue‐coloured berries involving regulation of both cyanidin and delphinidin branches.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

MYBA and MYBPA transcription factors co‐regulate anthocyanin biosynthesis in blue‐coloured berries

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…B-type PAs are widely distributed in plants and foods, such as senna alata leaves, pine trees, black wattle, beans, barley, sorghum, seeds, fruits, berries, nuts, cinnamon, chocolate, and wine [39][40][41][42][43][44][45]. A-type PAs are mainly found in bilberry, peanuts, plums, cranberries, curry, and cinnamon [46][47][48][49]. accumulation of PAs is associated with the resistance of plants against various biotic and abiotic stimuli, such as low temperature [27,28], drought [29], wounding [30,31], UV radiation [30], and fungal pathogens [32][33][34].…”

Section: Structure and Biosynthesis Of Proanthocyanidinsmentioning

confidence: 99%

“…B-type PAs are widely distributed in plants and foods, such as senna alata leaves, pine trees, black wattle, beans, barley, sorghum, seeds, fruits, berries, nuts, cinnamon, chocolate, and wine [39][40][41][42][43][44][45]. A-type PAs are mainly found in bilberry, peanuts, plums, cranberries, curry, and cinnamon [46][47][48][49]. The pathway of flavonoid biosynthesis has been well studied in various plants (Figure 2).…”

Section: Structure and Biosynthesis Of Proanthocyanidinsmentioning

confidence: 99%

Advances in Biosynthesis and Biological Functions of Proanthocyanidins in Horticultural Plants

Huang

Tian

et al. 2020

Foods

View full text Add to dashboard Cite

Proanthocyanidins are colorless flavonoid polymers condensed from flavan-3-ol units. They are essential secondary plant metabolites that contribute to the nutritional value and sensory quality of many fruits and the related processed products. Mounting evidence has shown that the accumulation of proanthocyanidins is associated with the resistance of plants against a broad spectrum of abiotic and biotic stress conditions. The biosynthesis of proanthocyanidins has been examined extensively, allowing for identifying and characterizing the key regulators controlling the biosynthetic pathway in many plants. New findings revealed that these specific regulators were involved in the proanthocyanidins biosynthetic network in response to various environmental conditions. This paper reviews the current knowledge regarding the control of key regulators in the underlying proanthocyanidins biosynthetic and molecular mechanisms in response to environmental stress. Furthermore, it discusses the directions for future research on the metabolic engineering of proanthocyanidins production to improve food and fruit crop quality.

show abstract

Dynamic changes in proanthocyanidin composition, biosynthesis, and histochemistry in spine grape (Vitis davidii Foëx) tissues during berry development

Yan,

Li,

Zheng

et al. 2024

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUNDSpine grapes are widely cultivated in southern China because of their strong adaptability to hot and humid climates. As a wild species native to China, spine grape (Vitis davidii Foëx) was studied as a resource of proanthocyanidins (PAs). PA composition, biosynthesis, and histochemistry in different tissues (skins, seeds, and stems) during berry development were analyzed in this study.RESULTSThe findings revealed that PA accumulation occurred in concurrence with flowering and was completed by veraison. High‐performance liquid chromatographic results showed that the epicatechin type was the most dominant. The skins were more likely to accumulate PA polymers. Reverse transcription–quantitative polymerase chain reaction analysis revealed that the expression levels of structural genes (flavonoid‐3′‐hydroxylase, flavonoid‐3′5′‐hydroxylase, dihydroflavonol 4‐reductase, leucoanthocyanidin reductase, and leucoanthocyanidin dioxygenase) were positively associated with PA dynamic changes. Histochemical results revealed that PAs in skins were mainly found in the hypodermis of the exocarp, PAs in seeds were mainly found in the middle layer of the outer integument of the testa, and PAs in stems were mainly found in the phloem.CONCLUSIONThis study provides a clear understanding of the spatial and temporal accumulation of PAs in spine grape, and forms a basis for the analysis of structural profiles and synthesis of PAs and their biological effects. © 2024 Society of Chemical Industry.

show abstract

Changes in the Proanthocyanidin Composition and Related Gene Expression in Bilberry (Vaccinium myrtillusL.) Tissues

Cited by 19 publications

References 43 publications

MYBA and MYBPA transcription factors co‐regulate anthocyanin biosynthesis in blue‐coloured berries

MYBA and MYBPA transcription factors co‐regulate anthocyanin biosynthesis in blue‐coloured berries

Advances in Biosynthesis and Biological Functions of Proanthocyanidins in Horticultural Plants

Dynamic changes in proanthocyanidin composition, biosynthesis, and histochemistry in spine grape (Vitis davidii Foëx) tissues during berry development

Contact Info

Product

Resources

About